Marine Environment Protection

Recent studies on residual malachite green in aquatic products

  • XU Xiang-rong ,
  • HAO Qing ,
  • PENG Jia-xi ,
  • SUN Yu-xin
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  • 1. Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China

Received date: 2012-09-28

  Revised date: 2012-12-16

  Online published: 2013-10-30

Abstract

Malachite green is a triphenylmethane bactericide and antiseptic used extensively in aquaculture due to its low cost and high efficiency on disease prevention. Because of the carcinogenicity, mutagenicity and teratogenicity of its parent compound and metabolites, malachite green and leucomalachite green bring potential harms to human health. This paper reviews recent progresses on the sources, toxicity effects, mechanisms, metabolic pathway, and detection methods of malachite green in aquatic products. Finally, some preventive measures to reduce the hazards of residual malachite green have been suggested. The complete ban of malachite green in aquaculture and rapid development of detection methods will greatly reduce the risks of malachite green in food security. This review is aimed at providing some updated information on the monitoring of residual malachite green in aquatic products and offering scientific and available suggestions to prevent and eliminate the hazards of malachite green in aquaculture.

Cite this article

XU Xiang-rong , HAO Qing , PENG Jia-xi , SUN Yu-xin . Recent studies on residual malachite green in aquatic products[J]. Journal of Tropical Oceanography, 2013 , 32(4) : 97 -106 . DOI: 10.11978/j.issn.1009-5470.2013.04.015

References

[1]HASHIMOTO J C, PASCHOAL J A R, DE QUEIROZ J F, et al. Considerations on the use of malachite green in aquaculture and analytical aspects of determining the residues in fish: a review[J]. Journal of Aquatic Food Product Technology, 2011, 20(3): 273-294.
[2]赵云峰, 吴永宁, 高俊全, 等. 中国居民膳食中农药残留的研究[J]. 中国流行病学杂志, 2003, 24: 661-664.
[3]NAKATA H, KAWAZOE M, ARIZONO K, et al. Organochlorine pesticides and polychlorinated biphenyl residues in foodstuffs and human tissues from China: Status of contamination, historical trend and human dietary exposure[J]. Archives of Environmental Contamination and Toxicology, 2002, 43: 473-480.
[4]张毅. 我国水产品出口额连续十年居全球首位[N]. 人民日报, 2012-6-19(1).
[5]黄裕勇. 穗市场桂花鱼几绝迹[N]. 大公报, 2012-6-4 (A6).
[6]CULP S J, BELAND F A. Malachite green: a toxicological review[J]. Journal of the American College of Toxicology, 1996, 15(3): 219-238.
[7]ALDERMAN D J. Malachite green: a review[J]. Journal of Fish Diseases, 1985, 8(3): 289-298.
[8]SCHNICK R A. The impetus to register new therapeutants for aquaculture[J]. Progressive Fish-Culturist, 1988, 50(4): 190-196.
[9]CULP S J, MELLICK P W, TROTTER R W, et al. Carcinogenicity of malachite green chloride and leucomalachite green in B6C3F1 mice and F344 rats[J]. Food and Chemical Toxicology, 2006, 44(8): 1204-1212.
[10]彭贞, 郑玉琴. 孔雀石绿与蛋白质相互作用的电化学行为的研究[J]. 化学试剂, 2009, 31(1): 49-51.
[11]孙满义, 杨贤庆, 岑剑伟, 等. 水产品中孔雀石绿的研究进展[J]. 食品工业科技, 2009, 30(2): 312-315.
[12]张彤晴, 周刚, 林海, 等. 孔雀石绿溶液水体自然降解初步研究[J]. 江苏农业科学, 2007, 1: 211-214.
[13]BERGWERFF A A, KUIPER R V, SCHERPENISSE P. Persistence of residues of malachite green in juvenile eels (Anguilla anguilla)[J]. Aquaculture, 2004, 233: 55-63.
[14]任倩, 蒋丽娟, 宋炜, 等. 孔雀石绿降解菌M3的分离鉴定及降解特性研究[J]. 生态与农村环境学报, 2007, 23(3): 65-69.
[15]LI LIANTAI, HONG QING, YAN XIN, et al. Isolation of a malachite green-degrading Pseudomonas sp. MDB-1 strain and cloning of the tmr2 gene[J]. Biodegradation, 2009(20): 769-776.
[16]林群, 梁旭方, 王琳, 等. 孔雀石绿生态毒理学与水产品污染控制技术研究进展[J]. 环境与健康杂志, 2008, 25(7): 654-657.
[17]周立红, 陈学豪, 徐长安. 用微核技术研究孔雀石绿对鱼的诱变作用[J]. 集美大学学报, 1997, 2(2): 55-57.
[18]SRIVASTAVA S, SINHA R, ROY D. Toxicological effects of malachite green[J]. Aquatic Toxicology, 2004, 66(3): 319-329.
[19]CULP S J, BLANKENSHIP L R, KUSEWITT D F, et al. Toxicity and metabolism of malachite green and leucomalachite green during short-term feeding to Fischer 344 rats and B6C3F(1) mice[J]. Chemico-Biological Interactions, 1999, 122(3): 153-170.
[20]BILLS T D, MARKING L L, CHANDLER J H Jr. Malachite green: its toxicity to aquatic organisms, persistence and removal with activated carbon[J]. Investigations Fish Control, 1977, 75: 1-6.
[21]李红霞, 那广水, 伍筱琳, 等. 孔雀石绿对两种藻类的影响[J]. 环境化学, 2009, 28(2): 225-228.
[22]黄英, 柯才焕, 周时强. 几种药物对波部东风螺早期发育的影响[J]. 厦门大学学报: 自然科学版, 2001, 40(3): 821-826.
[23]GERUNDO N, ALDERMAN D J, FEIST S W, et al. Pathological effects of repeated doses of malachite green: a preliminary study[J]. Journal of Fish Diseases, 1991, 14(5): 521-532.
[24]MEYER F P, JORGENSEN T A. Teratological and other effects of malachite green on development of rainbow trout and rabbits[J]. Transactions of The American Fisheries Society, 1983, 112(6): 818-824.
[25]RAO K V K. Inhibition of DNA synthesis in primary rat hepatocyte cultures by malachite green: a new liver tumor promoter[J]. Toxicology Letters, 1995, 81(2-3): 107-113.
[26]CULP S J, BELAND F A, HEXICH R H, et al. Mutagenicity and carcinogenicity in relation to DNA adduct formation in rats fed leucomalachite green[J]. Mutation Research, 2002, 506(sl): 55-63.
[27]RAO K V K, DAISY M M, ALKA A R. Malignant transformation of Syrian Hamster Embryo (SHE) cells in primary culture by malachite green: Transformation is associated with abrogation of G2/M checkpoint control[J]. Cell Biology International, 1998, 22, (7-8): 581-589.
[28]PANANDIKER A, FERNANDES C, RAO T K G, et al. Morphological transformation of syrian hamster embryo cells in primary culture by malachite green correlates well with the evidence for formation of reactive free radicals[J]. Cancer Letters, 1993, 74(1-2): 31-36.
[29]杨先乐, 喻文娟, 王民权, 等. 对孔雀石绿的禁用及其思考[J]. 水产科技情报, 2005, 32(5): 210-213.
[30]张佳燕, 伍金娥, 常超. 孔雀石绿的毒理学研究进展[J]. 粮食科技与经济, 2011, 36(3): 43-47.
[31]FORGACS E, CSERHATI T, OROS G. Removal of synthetic dyes from wastewaters: a review[J]. Environment International, 2004, 30(7): 953-971.
[32]VAN DER ZEE F P, VILLAVERDE S. Combined anaerobic- aerobic treatment of azo dyes-a short review of bioreactor studies[J]. Water Research, 2005, 39(8): 1425-1440.
[33]吴学立. 水产品中孔雀石绿、结晶紫及其代谢产物多残留检测的液相色谱-质谱法研究[D]. 武汉: 华中农业大学, 2007: 1-75.
[34]那广水, 李红霞, 周传光, 等. 高效液相色谱-串联质谱法测定海水中孔雀石绿及其代谢物[J]. 海洋环境科学, 2008, 27(2): 94-96.
[35]王恒. 紫外/荧光检测快速测定水产品中孔雀石绿、结晶紫及其代谢物[J]. 中国卫生检验杂志, 2010, 20(9): 2151-2153.
[36]POE W E, WILSON R P. Absorption of malachite green by channel catfish[J]. Progressive Fish-Culturist, 1983, 45(4): 228-229.
[37]MITROWSKA K, POSYNIAK A, ZMUDZKI J. Determination of malachite green and leucomalachite green in carp muscle by liquid chromatography with visible and fluorescence detection[J]. Journal of Chromatography A, 2005, 1089(1-2): 187-192.
[38]SRIVASTAVA A K, SINHA R, SINGH N, et al. Malachite green induced changes in carbohydrate metabolism and blood chloride levels in the freshwater catfish, Heteropneustes fossilis (Bloch)[J]. Acta Hydrobiologica, 1995, 37(2): 113-119.
[39]MEINELT T, PLAYLE R, SCHRECKENBACH K, et al. The toxicity of the antiparasitic mixture, FMC is changed by humic substances and calcium[J]. Aquaculture Research, 2001, 32(5): 405-410.
[40]PLAKAS S M, EISAID K R, STEHLY G R, et al. Uptake, tissue distribution, and metabolism of malachite green in the channel catfish (Ictalurus punctatus)[J]. Canadian Journal of Fisheries and Aquatic Sciences, 1996, 53(6): 1427-1433.
[41]TURNIPSEED S B, ROYBAL J E, HURLBUT J A, et al. Gas chromatographic mass spectrometric confirmation of leucomalachite green in catfish (Ictalurus punctatus) tissue[J]. Journal of AOAC International, 1995, 78(4): 971- 977.
[42]CULP S J, BLANKENSHIP L R, KUSEWITT D F, et al. Metabolic changes occurring in mice and rats fed leucomalachite green, a reduced derivative of the antifungal agent malachite green[C]. San Diego, California, USA: 88th Annual Meeting of American Association for Cancer Research, 1997: 129.
[43]陶艳玲, 邓爱妮, 刘秋梅, 等. 固相微萃取-高效液相色谱法快速测定水中孔雀石绿[J]. 理化检验-化学分册, 2011, 47(9): 1092-1094.
[44]ALBORALI L, SANGIORGI E, LEALI M, et al. The persistence of malachite green in the edible tissue of rainbow trout (Oncorhynchus mykiss)[J]. Italian Journal of Aquaculture -Rivista Italiana di Acquacoltura, 1997, 32(2): 45-60.
[45]ROYBAL J E, PFENNING A P, MUNNS R K, et al. Determination of malachite green and its metabolite, leucomalachite green, in catfish (Ictalurus punctatus) tissue by liquid-chromatography with visible detection[J]. Journal of AOAC International, 1995, 78(2): 453-457.
[46]邱绪建, 林洪, 王联珠, 等. 孔雀石绿及其代谢物隐性孔雀石绿在鲫鱼肌肉中的代谢规律[J]. 中国海洋大学学报: 自然科学版, 2006, 36(5): 745-748.
[47]高露姣, 蔡友琼, 姜朝军, 等. 孔雀石绿及其主要代谢产物在欧洲鳗鲡肌肉中蓄积及消除规律[J]. 水产学报, 2007, 31(增刊): 104-108.
[48]曲志娜, 李存金, 赵思俊, 等. 孔雀石绿及其代谢物在大菱鲆肌肉中的消除规律[J]. 中国农学通报, 2008, 24(6): 491-496.
[49]杨贤庆, 孙满义, 岑剑伟, 等. 孔雀石绿及其代谢产物在罗非鱼肌肉中残留规律的研究[J]. 热带海洋学报, 2010, 29(4): 107-111.
[50]周兵, 陈璐, 李英华. 几种水产品中隐性孔雀石绿残留量的测定[J]. 广州化工, 2011, 39(21): 115-118.
[51]罗瑞峰, 罗小玲, 马小宁. 水产品中孔雀石绿、结晶紫及其代谢产物检测方法的探讨[J]. 化学分析计量, 2011, 20(3): 40-42.
[52]吴淑秀, 公茂金, 刘玉芳, 等. HPLC-MS/MS 测定活泥鳅中孔雀石绿、结晶紫及其代谢物残留[J]. 现代农业科技, 2010, 20: 340, 345.
[53]王丽敏, 杜雪莉, 赵艳, 等. 超高效液相色谱-串联质谱快速测定水产品中孔雀石绿、结晶紫及其代谢物残留量[J]. 南方水产, 2010, 6(4): 32-36.
[54]赵全东, 戴欣. 水产品中孔雀石绿及其代谢物残留量检测方法研究进展[J]. 吉林水利, 2012, 1(1): 37-39.
[55]桂英爱, 王洪军, 马琳, 等. 高效液相色谱法同时测定海水中孔雀石绿和隐性孔雀石绿[J]. 环境监测管理与技术, 2008, 20(2): 44-45.
[56]王丽玲, 冯翠霞, 胡尔萍. 水样中孔雀石绿残留量测定方法探讨[J]. 中国卫生检验杂志, 2007, 17(10): 1809-1810.
[57]朱宽正, 王鹏, 林雁飞, 等. 液相色谱-串联质谱法同时测定水产品中孔雀石绿、结晶紫以及它们的隐色代谢物残留[J]. 色谱, 2007, 25(1): 26-29.
[58]郑斌, 赵红萍, 冷凯良, 等. 高效液相色谱-荧光检测法测定鳗鲡中孔雀石绿和结晶紫残留[J]. 中国水产科学, 2007, 14(6): 1038-1041.
[59]郭德华, 叶长淋, 朱莹洁. 液相色谱法测定鳗鱼中的孔雀石绿[J]. 化学分析计量, 2002, 11(2): 20-21.
[60]RUSHING L G, THOMPSON H C. Simultaneous determination of malachite green, gentian violet and their leuco metabolites in catfish or trout tissue by high performance liquid chromatography with visible detection[J]. Journal of Chromatography B, 1997, 688(2): 325-330.
[61]HALME K, LINDFORS E, PELTONEN K. Determination of malachite green residues in rainbow trout muscle with liquid chromatography and liquid chromatography coupled with tandem mass spectrometry[J]. Food Additives and Contaminants, 2004, 21, (7): 641-648.
[62]白丽丽, 林正峰, 戴华, 等. HPLC法检测水产品中孔雀石绿、结晶紫及其关联化合物的残留[J]. 海南医学院学报, 2011, 17(9): 1157-1160.
[63]陈建伟, 姚志云, 毛健伟, 等. 浊点萃取-高效液相色谱法测定虾肉中孔雀石绿和结晶紫残留的研究[J]. 南京农业大学学报, 2010, 33(1): 94-98.
[64]RUSHING L G, HANSEN E B. Confirmation of malachite green, gentian violet and their leuco analogs in catfish and trout tissue by high-performance liquid chromatography utilizing electrochemistry with ultraviolet-visible diode array detection and fluorescence detection[J]. Journal of Chromatography B, 1997, 700(1-2): 223-231.
[65]BERGWERFF A A, SCHERPENISSE P. Determination of residues of malachite green in aquatic animals[J]. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2003, 788(2): 351-359.
[66]任秀莲, 魏琦峰, 曲径, 等. 反相高效液相色谱法测定水产品中孔雀石绿、结晶紫及其代谢物[J]. 中国卫生检验杂志, 2006, 16(8): 939-940.
[67]SAFARIK I, SAFARIKOVA M. Detection of low concentrations of malachite green and crystal violet in water[J]. Water Research, 2002, 36(1): 196-200.
[68]徐彦辉, 顾亮, 蒋俊树, 等. 液相色谱串联质谱法测定养殖水体中孔雀石绿及其代谢物[J]. 化学分析计量, 2011, 20(2): 49-51.
[69]VALLE L, DIAZ C, ZANOCCO A L, et al. Determination of the sum malachite green and leucomalachite green in salmon muscle by liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry[J]. Journal of Chromatography A, 2005, 1067(1-2): 101-105.
[70]郭德华, 叶长淋, 李波, 等. 高效液相色谱-质谱法测定水产品中孔雀石绿及其代谢物[J]. 分析测试学报, 2004, 23(增刊): 206-208.
[71]余培建. 高效液相色谱法检测底泥中孔雀石绿及其代谢物[J]. 化学分析计量, 2007, 16(2): 27-29.
[72]岑剑伟, 杨贤庆, 李来好, 等. 用高效液相色谱法检测底泥环境中孔雀石绿的方法[J]. 大连水产学院学报, 2009, 24(6): 568-573.
[73]MORTEZA B, FOROOGH K, PEYMAN N M. Development of cloud point extraction using pH-sensitive hydrogel for preconcentration and determination of malachite green[J]. Talanta, 2011, 85(2): 891-896.
[74]POURREZA N, ELHAMI S. Spectrophtometric determination of malachite green in fish farming water samples after cloud point extraction using nonionic surfactant Triton X-100[J]. Analytica Chimica Acta, 2007, 596(1): 62-65.
[75]莫利, 杨迎春, 韩静, 等. 分散液液微萃取-分光光度法测定环境水样中孔雀石绿[J]. 光谱实验室, 2011, 28(1): 205-209.
[76]HAJEE C A J, HAAGSMA N. Simultaneous determination of malachite green and its metabolite leucomalachite green in eel plasma using postcolumn oxidation[J]. Journal of Chromatography B, 1995, 699(2): 219-227.
[77]赵东豪, 黎智广, 林钦, 等. 高效液相色谱串联质谱测定沉积物中的孔雀石绿、结晶紫及其隐色代谢物[J]. 广东农业科学, 2011(2): 171-173.
[78]肖琴, 林一, 郑琳, 等. 分子印迹固相萃取-高效液相色谱法检测鱼肉中的孔雀石绿和结晶紫[J]. 粮油食品科技, 2011, 19(6): 31-34.
[79]邱绪建. 鱼体内孔雀石绿残留检测方法及代谢的研究[D]. 青岛: 中国海洋大学, 2004: 1-59.
[80]SCHERPENISSE P, BERGWERFF A A. Determination of residues of malachite green in finfish by liquid chromatography tandem mass spectrometry[J]. Analytica Chimica Acta, 2005, 529(1-2): 173-177.
[81]林谦, 林炜, 叶瑞洪. 紫外可见分光光度法测定水产品中孔雀石绿含量[J]. 福建师范大学福清分校学报, 2009, 94(5): 60-64.
[82]顾振华, 赵宇翔, 吴卫平, 等. 表面增强拉曼光谱法快速检测水产品中的孔雀石绿[J]. 化学世界, 2011, 1: 14-16.
[83]张海琪, 何中央, 郑重莺, 等. 液相色谱-串联质谱法测定水产品中孔雀石绿的残留量[J]. 南方水产, 2007, 3(6): 14-21.
[84]TSAI C H, LIN J D, LIN C H. Optimization of the separation of malachite green in water by capillary electrophoresis raman spectroscopy (CE-RS) based on the stacking and sweeping modes[J]. Talanta, 2007, 72(2): 368-372.
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