Marine Biology

The effects of different diet protein sources on carbon and nitrogen isotope fractionation of juvenile cobia Rachycentron canadum L.

  • ZHOU Hui ,
  • CHEN Gang ,
  • SHI Gang ,
  • ZHANG Jian-dong ,
  • DONG Xiao-hui
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  • 1. Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China; 2. Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal, Regular High Education Institute of Guangdong Province, Zhanjiang 524025, China

Received date: 2013-06-24

  Revised date: 2013-09-20

  Online published: 2014-09-29

Abstract

In order to investigate the effects of different diet protein sources on carbon and nitrogen isotope fractionation of juvenile cobia Rachycentron canadum L., three isonitrogenous and isocaloric diets were formulated. The protein source in D1 was fish meal; beer yeast meal and corn gluten meal protein replaced 10% fish meal in D2 and D3, respectively. The diets were fed to juvenile cobia for 24 days. The results showed that, when 10% fish meal was replaced, the weight gain rate (WGR) of cobia decreased significantly. The carbon isotope ratio δ13C of cobia increased in time and nitrogen isotope ratio δ15N decreased in time in all groups. Although the change speeds of δ15N were different, the whole fish and muscle of cobia fed with different diets reached isotope equilibrium states with their diets when the feeding experiment was accomplished. When 10% fish meal was replaced by beer yeast meal (corn gluten meal) protein, the carbon isotope fractionation Δ13C decreased while nitrogen isotope fractionation Δ15N increased in the whole fish and muscle of cobia; Δ13C of the whole fish decreased from 4.19‰ to 3.94‰ and 3.63‰, Δ13C of the muscle decreased from 4.46‰ to 3.98‰ (to 3.67‰), and Δ15N of the whole fish increased from 0.18‰ to 0.88‰ (to 0.94‰), Δ15N of the muscle increased from 0.18‰ to 0.74‰ (to 0.87‰). When the three diets with different protein sources were fed, the variation trends of Δ13C and Δ15N were similar between whole fish and muscle of cobia, but the change speed of whole fish δ15N was slower than that of muscle. These results indicated that cobia muscle can represent the δ13C and δ15N characters of whole fish in trophic level study of ecosystem (long time scale), but cannot represent the change process of whole fish δ15N in metabolic physiology study (short time scale).

Cite this article

ZHOU Hui , CHEN Gang , SHI Gang , ZHANG Jian-dong , DONG Xiao-hui . The effects of different diet protein sources on carbon and nitrogen isotope fractionation of juvenile cobia Rachycentron canadum L.[J]. Journal of Tropical Oceanography, 2014 , 33(5) : 35 -40 . DOI: 10.11978/j.issn.1009-5470.2014.05.005

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