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Journal of Tropical Oceanography >

2015 , Vol. 34 >Issue 1: 83 - 89

DOI: https://doi.org/10.11978/j.issn.1009-5470.2015.01.012

Marine Biology

Molecular cloning and sequence analysis of the copper-zinc superoxide dismutase gene in Galaxea astreata

  • FAN Cheng-hui ,
  • LIU Li ,
  • SHEN Cheng ,
  • GUO Yu-song
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  • Fisheries College, Guangdong Ocean University, Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Zhanjiang 524088, China

Received date: 2014-03-24

  Revised date: 2014-06-14

  Online published: 2015-02-10

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Abstract

The full-length cDNA sequence of copper-zinc superoxide dismutase (CuZnSOD) gene was obtained from Galaxea astreata using RT-PCR and RACE methods. The results revealed that the full-length cDNA of CuZnSOD gene was 730 bp including 111 bp 5°-UTR, 188 bp 3°-UTR and 471 bp open reading frame (ORF) encoding 156 amino acids. The deduced amino acid sequence contained two CuZnSOD protein family signals. Cu2+ integrated with His49, His51, His66, and His123, and Zn2+ integrated with His66, His74, His83, and Asp86. The only intrachain disulfide bond was formed between Cys60 and Cys149. The signal peptides were not predicted by SignalP 3.0 Server, the transmembrane domains were not predicted by TMHMM Server v. 2.0, and the possibility of 56.5% was located in the cytoplasm by means of PSORT II Prediction, indicating that the deduced protein was intracellular CuZnSOD. The results will help to further reveal antioxidant molecular mechanism in Sceratinian corals.

Key words： CuZnSOD; gene cloning; sequence analysis; Galaxea astreata

Cite this article

FAN Cheng-hui , LIU Li , SHEN Cheng , GUO Yu-song . Molecular cloning and sequence analysis of the copper-zinc superoxide dismutase gene in Galaxea astreata[J]. Journal of Tropical Oceanography, 2015 , 34(1) : 83 -89 . DOI: 10.11978/j.issn.1009-5470.2015.01.012

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