The secondary metabolites of strain ZSDS1-F11, an endophytic fungus isolated from the sponge Phakelliafusca Thiele, were investigated. Fermented products of ZSDS1-F11 were purified by means of chromatography over silica gel column, Sephadex LH-20 and semi-preparative HPLC. Compounds were confirmed based on their physical and chemical properties, spectroscopic analysis and comparison with the relevant literature. Using COX-1 and COX-2 as molecular targets for inflammation inhibitor screening, eight compounds were obtained from the fermented products of strain ZSDS1-F11. Compounds 1 and 2 showed weak inhibitory activity against enzyme COX-1, their IC50 values were 27.2 and 2.93 μmol, respectively. The strain ZSDS1-F11 produced diverse bioactive secondary metabolites. This is the first report on compounds 1 and 2 with weak anti-inflammatory activity.
HE Wei-jun
,
WANG Jun-feng
,
LIN Xiu-ping
,
ZHOU Xiao-jiang
,
LIU Yong-hong
. Study on the secondary metabolites from a sponge-derived fungus ZSDS1-F11[J]. Journal of Tropical Oceanography, 2015
, 34(5)
: 36
-39
.
DOI: 10.11978/2015011
1 韩秀丽, 林贞健, 陶洪文, 等. 2009. 红海榄共生真菌 Penicillium sp. HK13-8细胞毒活性成分研究[J]. 中国海洋药物, (5): 11-16.
2 李益, 唐金山, 高昊, 等. 2010. 海洋放线菌 Micromonospora sp.(No. 69) 抗 MRSA 活性成分研究[J]. 中国海洋药物, (5): 16-21.
3 宋恺, 胡洁, 林文翰, 等. 2014. 海绵共附生真菌多样性及其次级代谢产物的研究进展[J]. 生物技术通报, (04): 36-42.
4 王磊, 方玉春, 刘红兵, 等. 2005. 海绵共附生微生物活性次级代谢产物的研究进展[J]. 中国海洋药物, (06): 52-56.
5 CHU DAN, PENG CHONGSHENG, DING BO, et al . 2011. Biological active metabolite cyclo (L-Trp-L-Phe) produced by South China Sea sponge Holoxea sp. associated fungus Aspergillus versicolor strain TS08[J]. Bioprocess and Biosystems Engineering, 34(2): 223-229.
6 DING ZHANGGUI, ZHAO JIANGYUAN, YANG PEIWEN, et al. 2009. 1 H and 13 C NMR assignments of eight nitrogen containing compounds from Nocardia alba sp.nov (YIM 30243 (T))[J]. Magnetic Resonance in Chemistry: MRC, 47(4): 366-370.
7 GHISALBERTI E, HOCKLESS D, ROWLAND C, et al. 1993. Structural study of curvularin, a cell division inhibitor[J]. Australian Journal of Chemistry, 46(4): 571-575.
8 GUTIERREZ-LUGO M T, WOLDEMICHAEL G M, SINGH M P, et al . 2005. Isolation of three new naturally occurring compounds from the culture of Micromonospora sp. P1068[J]. Natural Product Research, 19(7): 645-652.
9 KIMURA Y, TANI K, KOJIMA A, et al . 1996. Cyclo- (l-tryptophyl-l-phenylalanyl), a plant growth regulator produced by the fungus Penicillium sp[J]. Phytochemistry, 41(3): 665-669.
10 LIU RONGFENG, ZHANG DAOJING, LI YUANGUANG. 2010. A new antifungal cyclic lipopeptide from Bacillus marinus B-9987[J]. Helvetica Chimica Acta, 93: 2419-2425.
11 PORNPAKAKUL S, LIANGSAKUL J, NGAMROJANAVANICH N, et al. 2006. Cytotoxic activity of four xanthones from Emericella variecolor , an endophytic fungus isolated from Croton oblongifolius [J]. Archives of Pharmacal Research, 29(2): 140-144.
12 PROKSCH P, EDRADA R, EBEL R, et al. 2002. Drugs from the seas-current status and microbiological implications[J]. Applied Microbiology and Biotechnology, 59(2/3): 125-134.
13 WANG SHUANGMING, TAN NINGHUA, YANG YABIN, et al . 2003. Cyclodipeptides from the roots of Panax notoginseng [J]. Natural Product Research and Development, 16(5): 383-386.
14 ZHANG XIAOYING, ZHAO QUANYU, XUE SONG, et al. 2002. Bioactive compounds from marine sponges and cell culture of marine sponges[J]. Chinese Journal of Biotechnology, 18(1): 10-15.
