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Journal of Tropical Oceanography >

2016 , Vol. 35 >Issue 4: 63 - 70

DOI: https://doi.org/10.11978/2015125

Marine Biology

Cloning, expression and characterization of a thermo-tolerant manganese superoxide dismutase from Bacillus sp. SCSIO 15029

  • DENG Dun ,
  • ZHANG Yun ,
  • SUN Aijun ,
  • HU Yunfeng
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  • 1. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China;
    3. Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China

Received date: 2015-10-13

  Online published: 2016-08-04

Supported by

Strategic Priority Research Program of the Chinese Academy of Sciences (XDA11030404)

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Abstract

A putative superoxide dismutase gene (BaSOD) was cloned from Bacillus sp. SCSIO 15029, which was isolated from the sediment of the South China Sea. The BaSOD gene was expressed in Escherichia coli BL21(DE3) by using pET-28a(+) expression system. The recombinant enzyme was soluble in E. coli, and its biochemical properties were studied. BaSOD exhibits maximum activity at pH 7.5 and 40℃ with a specific activity of BaSOD at 5511.46 U·mg-1. BaSOD is a manganese-dependent superoxide dismutase. The enzyme activity was basically not affected after incubation at 60℃ for 30 min. The residual activity was 71% after incubation at 70℃ for 30 min. BaSOD shows good thermo-stability and possesses very good potential for industrial utilization.

Key words： Bacillus; deep-sea thermophilic enzyme; superoxide dismutase

Cite this article

DENG Dun , ZHANG Yun , SUN Aijun , HU Yunfeng . Cloning, expression and characterization of a thermo-tolerant manganese superoxide dismutase from Bacillus sp. SCSIO 15029[J]. Journal of Tropical Oceanography, 2016 , 35(4) : 63 -70 . DOI: 10.11978/2015125

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