Marine Biology

Gene cloning and analysis of a novel C-type lectin PhLecA from Panulirus homaus

  • LI Bin ,
  • LIANG Huafang ,
  • CHAN Siuming ,
  • HUANG Qixing ,
  • CHENG Zhifen ,
  • CHEN Shikun
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  • Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China

Received date: 2015-12-24

  Online published: 2016-09-22

Supported by

Foundation item: This study funded by the Special Project of Science and Technology for Marine Fisheries of Guangdong Province (A201208D04)

Abstract

C-type lectins (CTLs) are a large family of proteins that exist in all deuterostomia. CTLs can bind to carbohydrate moieties normally in a calcium-dependent manner and play important roles in immune defense. The cDNA sequence of Panulirus homaus LecA (PhLecA) was obtained using rapid amplification of cDNA ends method (RACE). The full-length cDNA sequence of PhLecA was 863 bp, which contained a 729 bp complete open reading frame (ORF) encoding 243 amino acids. The deduced amino acid sequence of PhLecA had a signal peptide containing 17 amino acid residues and a carbohydrate recognition domain (CRD). RT-PCR analysis showed that the mRNA of PhLecA was widely distributed in various tissues in the embryonic period and the larval stage, with highest expression in hepatopancreas and then in stomach, intestine and eye pigment formation stage. Real-time PCR analysis indicated that PhLecA transcripts level showed significant change in hepatopancreas after the lobster was artificially challenged with white spot syndrome virus (WSSV) and Vibrio harveyi, the overall change trend of PhLecA transcripts first went up, and then went down to normal level. The results suggested that PhLecA might be involved in the immune response against virus and bacterial.

Cite this article

LI Bin , LIANG Huafang , CHAN Siuming , HUANG Qixing , CHENG Zhifen , CHEN Shikun . Gene cloning and analysis of a novel C-type lectin PhLecA from Panulirus homaus[J]. Journal of Tropical Oceanography, 2016 , 35(5) : 29 -37 . DOI: 10.11978/2015155

References

1 陈昌生, 纪德华, 吴坤杰, 等, 2003. 波纹龙虾人工繁殖及早期叶状幼体培育的初步研究[J]. 集美大学学报(自然科学版), 8(3): 197-202. CHEN CHANGSHENG, JI DEHUA, WU KUNJIE, et al, 2003. The preliminary research on the artificial reproduction and culture of the phyllosomas of Panulirus homarus [J]. Journal of Jimei University (Natural Science), 8(3): 197-202 (in Chinese).
2 梁华芳, 徐晓鹏, 黄志坚, 等, 2011. 中国沿海龙虾属8种龙虾COI基因序列的分子系统学研究[J]. 中山大学学报(自然科学版), 50(6): 94-98. LIANG HUAFANG, XU XIAOPENG, HUANG ZHIJIAN, et al, 2011. The molecular phylogeny on eight species of Panulirus genus from the Chinese coast based on COI gene sequence[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 50(6): 94-98 (in Chinese).
3 梁华芳, 何建国, 2012. 锦绣龙虾生物学和人工养殖技术研究[M]. 北京: 海洋出版社: 5-10.
4 孙册, 1994. 谈谈凝集素作用的基本原理和糖蛋白的寡糖结构[J]. 生命的化学, 14(2): 36-37.
5 王显伟, 2012. 十足目甲壳动物C型凝集素功能研究[D]. 济南: 山东大学, 15-25. WANG XIANWEI, 2012. Functional study of C-type lectins from decapod crustaceans[D]. Ji’nan: Shandong University, 15-25(in Chinese).
6 胥炜, 王昊, 宋林生, 等, 2005. 栉孔扇贝C型凝集素基因的克隆与表达研究[J]. 高技术通讯, 15(1): 83-88. XU WEI, WANG HAO, SONG LINSHENG, et al, 2005. cDNA cloning and mRNA expression of C-type lectin from scallop Chlamys farreri [J]. Chinese High Technology Letters, 15(1): 83-88(in Chinese).
7 AKIRA S, TAKEDA K, KAISHO T, 2001. Toll-like receptors: critical proteins linking innate and acquired immunity[J]. Nature Immunology, 2(8): 675-680.
8 CLIVE J, 2010. Tropical rock lobster aquaculture development in Vietnam, Indonesia and Australia[J]. Journal of Marine Biological Association of India, 52(2): 304-315.
9 DRICKAMER K, 1988. Two distinct classes of carbohydrate- recognition domains in animal lectins[J]. The Journal of Biological Chemistry, 263(20): 9557-9560.
10 DRUMMOND R A, BROWN G D, 2013. Signalling C-type lectins in antimicrobial immunity[J]. PLoS Pathog, 9(7): e100341.
11 GEIJTENBEEK T B H, GRINGHUIS S I, 2009. Signalling through C-type lectin receptors: shaping immune responses[J]. Nature Reviews Immunology, 9(7): 465-479.
12 JIN XINGKUN, LI WEIWEI, CHENG LIN, et al, 2012. Two novel short C-type lectin from Chinese mitten crab, Eriocheir sinensis , are induced in response to LPS challenged[J]. Fish & Shellfish Immunology, 33(5): 1149-1158.
13 JUNKUNLO K, PRACHUMWAT A, TANGPRASITTIPAP A, et al, 2012. A novel lectin domain-containing protein (LvCTLD) associated with response of the whiteleg shrimp Penaeus ( Litopenaeus ) vannamei to yellow head virus (YHV)[J]. Developmental & Comparative Immunology, 37(3/4): 334-341.
14 KONG H J, PARK E M, NAM B H, et al, 2008. A C-type lectin like-domain (CTLD)-containing protein (PtLP) from the swimming crab Portunus trituberculatus [J]. Fish & Shellfish Immunology, 25(3): 311-314.
15 LIU YICHEN, LI FUHUA, DONG BO, et al, 2007. Molecular cloning, characterization and expression analysis of a putative C-type lectin (Fclectin) gene in Chinese shrimp Fenneropenaeus chinensis [J]. Molecular Immunology, 44(4): 598-607.
16 LUO TIAN, YANG HAIJIE, LI FANG, et al, 2006. Purification, characterization and cDNA cloning of a novel lipopolysaccharide- binding lectin from the shrimp Penaeus monodon [J]. Developmental & Comparative Immunology, 30(7): 607-617.
17 LUO TIAN, LI FANG, LEI KAIYU, et al, 2007. Genomic organization, promoter characterization and expression profiles of an antiviral gene PmAV from the shrimp Penaeus monodon [J]. Molecular Immunology, 44(7): 1516-1523.
18 MA T H T, TIU S H K, HE JIANGUO, et al, 2007. Molecular cloning of a C-type lectin (LvLT) from the shrimp Litopenaeus vannamei : Early gene down-regulation after WSSV infection[J]. Fish & Shellfish Immunology, 23(2): 430-437.
19 MA T H T, BENZIE J A H, HE JIANGUO, et al, 2008. PmLT, a C-type lectin specific to hepatopancreas is involved in the innate defense of the shrimp Penaeus monodon [J]. Journal of Invertebrate Pathology, 99(3): 332-341.
20 RAO XIANGJUN, SHAHZAD T, LIU SU, et al, 2015. Identification of C-type lectin-domain proteins (CTLDPs) in silkworm Bombyx mori [J]. Developmental and Comparative Immunology, 53(2): 328-338.
21 RATTANAPORN O, UTARABHAND P, 2011. Molecular cloning of a C-type lectin with two CRD domains from the banana shrimp Fenneropenaeus merguiensis : Early gene up-regulation after Vibrio harveyi infection[J]. Journal of Invertebrate Pathology, 106(2): 196-204.
22 REN QIAN, LI MENG, DU JIE, et al, 2012. Immune response of four dual-CRD C-type lectins to microbial challenges in giant freshwater prawn Macrobrachium rosenbergii [J]. Fish & Shellfish Immunology, 33(2): 155-167.
23 ROJTINNAKORN J, HIRONO I, ITAMI T, et al, 2002. Gene expression in haemocytes of Kuruma prawn, Penaeus japonicus , in response to infection with WSSV by EST approach[J]. Fish & Shellfish Immunology, 13(1): 69-83.
24 SHARON N, LIS H, 2004. History of lectins: from hemagglutinins to biological recognition molecules[J]. Glycobiology, 14(11): 53R-62R.
25 SUN JIE, WANG LEI, WANG BIAOJIE, et al, 2008a. Purification and characterization of a natural lectin from the plasma of the shrimp Fenneropenaeus chinensis [J]. Fish & Shellfish Immunology, 25(3): 290-297.
26 SUN YANDONG, FU LIDONG, JIA YUPING, et al, 2008b. A hepatopancreas-specific C-type lectin from the Chinese shrimp Fenneropenaeus chinensis exhibits antimicrobial activity[J]. Molecular Immunology, 45(2): 348-361.
27 SUPUNGUL P, KLINBUNGA S, PICHYANGKURA R, et al, 2002. Identification of immune-related genes in hemocytes of black tiger shrimp ( Penaeus monodon )[J]. Marine Biotechnology, 4(5): 487-894.
28 WANG XIANWEI, ZHANG XIAOWEN, XU WENTENG, et al, 2009. A novel C-type lectin (FcLec4) facilitates the clearance of Vibrio anguillarum in vivo in Chinese white shrimp[J]. Developmental & Comparative Immunology, 33(9): 1039-1047.
29 WANG XIANWEI, WANG JINXING, 2013. Diversity and multiple functions of lectins in shrimp immunity[J]. Developmental & Comparative Immunology, 39(1/2): 27-38.
30 WILLIAMMS K C, 2007. Nutritional requirements and feeds development for post-larval spiny lobster: A review[J]. Aquaculture, 263(1/4): 1-14.
31 YANG HAIJIE, LUO TIAN, LI FANG, et al, 2007. Purification and characterisation of a calcium-independent lectin (PjLec) from the haemolymph of the shrimp Penaeus japonicus [J]. Fish & Shellfish Immunology, 22(1/2): 88-97.
32 ZHANG HUAN, WANG HAO, WANG LINGLING, et al, 2009a. Cflec-4, a multidomain C-type lectin involved in immune defense of Zhikong scallop Chlamys farreri[J]. Developmental & Comparative Immunology, 33(6): 780-788.
33 ZHANG YING, QIU LIMEI, SONG LINSHENG, et al, 2009b. Cloning and characterization of a novel C-type lectin gene from shrimp Litopenaeus vannamei[J]. Fish & Shellfish Immunology, 26(1): 183-192.
34 ZHANG XIAOWEN, XU WENTENG, WANG XIANWEI, et al, 2009c. A novel C-type lectin with two CRD domains from Chinese shrimp Fenneropenaeus chinensis functions as a pattern recognition protein[J]. Molecular Immunology, 46(8-9): 1626-1637.
35 ZHAO ZHIYING, YIN ZHIXIN, XU XIAOPENG, et al, 2009. A novel C-type lectin from the shrimp Litopenaeus vannamei possesses anti-white spot syndrome virus activity[J]. Journal of Virology, 83(1): 347-356.
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