The bacterial compositions of surface seawater and sediment in Wei-zhou oil exploring field were investigated and compared by Illumina MiSeq high-throughput sequencing based on 16S rRNA gene in this study. Shannon diversity index values indicated that the bacterial community in Wei-zhou oil exploring field was highly diverse, and the bacterial diversity in the surface seawater samples was much higher than that in the sediment samples. The main bacterial group in the surface seawater samples was Bacilli, followed by Cyanobacteria, Alphaproteobacteria and Gammaproteobacteria, whereas Gammaproteobacteria dominated the sediment samples. Venn diagram demonstrated bacterial composition from the surface seawater and that from the sediment in Wei-zhou oil exploring field displayed high similarity. However, heat map and principal component analysis showed that the samples from seawater and those from the sediment clustered separately. Illumina MiSeq high-throughput sequencing also indicated that some potential oil-degradation bacteria, such as bacteria in genera of Pseudomonas, Sphingomonas, Rhodococcus, and Mycobacterium existed in this field. Furthermore, those four kinds of bacteria in the sediment were more abundant than in the surface seawater, suggesting that the oil concentration in the environment might play an important role in regulating oil-degradation bacterial community. This work provides some new insights toward the effects of oil exploring activities on bacterial community and oil biodegradation potential in marine environment.
WU Peng
,
LIAN Zhonglian
,
JIANG Zhongchen
,
DENG Wei
,
XIONG Xiaofei
,
SHI Xiaojun
,
ZHANG Jinghuai
,
LOU Quansheng
,
FANG Hongda
. Composition of bacterial community in Weizhou oil exploring field revealed by high-throughput sequencing[J]. Journal of Tropical Oceanography, 2016
, 35(5)
: 48
-54
.
DOI: 10.11978/2016003
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