Journal of Tropical Oceanography >
Morphology and histology of the digestive system in Girella leonina*
Received date: 2017-04-11
Request revised date: 2017-08-15
Online published: 2018-02-02
Supported by
Public Technology Application Research Projects of Zhejiang Province (2017C32015)
Copyright
The organizational structural characteristics of digestive system of fish are closely related to diet. To study the digestive physiological characteristics of reef fish Girella leonine, the organizational structural characteristics of digestive system were investigated by H.E staining. The correlation between the structural characteristics of tissue and the adaptability of diet and environment was discussed. The results are summarized as follows. 1) The esophagus tended to the cavity to form six thick folds, which had obvious striated muscle and mucous cells, and the folds had strongly expansion ability. Mucous cells were distributed between mucosa epithelium and were cone-shaped. 2) “V-shaped” stomach was divided into cardia, gastric body and pylorus. The transverse section of gastric gland was round and the lumen was surrounded by several gland cells. The muscular layer of the stomach was well developed, its inner was annulus structure and the outer was longitudinal structure. The stomach glands were mostly developed in the lamina of gastric body, the gastric mucosa formed many folds and the pyloric stomach had multi-level branch folds. 3) There are more than 150 branched diverticulum in the pyloric, and they can be divided into two branches. The cavity of the cecum was filled with mucosal folds. The epithelium of mucosa was composed of single columnar epithelial cells without goblet cells. 4) Liver was divided into two leaves, which showed a general structure of bony fish in histology. The liver cells were large and polygonal, with a size of nearly 30 μm. 5) The intestine had four convolutions, and the ratio of intestine length to body length was 1.52±0.06, the value was between herbivorous and carnivorous fish. There were lots of goblet cells distributing in the whole intestine. The intestine had well-developed villi of small intestine. The epithelium of the villus base caved into the lamina propria to form the intestinal gland. The gland opened to the adjacent villi base. As the main digestible site, the number and height of the villi were greater than those in the hindgut. In this study, we also discussed the relationship between the histology characteristic and the digestion and absorption of digestive system of Girella leonine. Results showed that the characteristics of digestive system of Girella leonina was typical of omnivorous fish digestive system.
Key words: Girella leonina; digestive system; algophagous; histology
CAO Xiao , SUN Min , ZHU Lianghua , ZHANG Dingyuan , XU Shanliang . Morphology and histology of the digestive system in Girella leonina*[J]. Journal of Tropical Oceanography, 2018 , 37(1) : 20 -26 . DOI: 10.11978/2017040
Fig. 1 Maps of the digestive system and stomach of Girella leonine. 1. Cardia department; 2. Pylorus department; 3. Blind sac department图1 黑鱾消化系统及胃的示意图 |
Fig. 2 The esophagus, stomach and pyloric caeca histology of Girella leonine. a. The esophagus crosscutting, → showing mucosal folds; b. a partially enlarged figure, showing esophageal cavity wall structures, ← showing mucous epithelium; c. body of stomach, →← showing a large number of gastric gland exist in the lamina propria; d. cardiac stomach, ↑ showing gastric pit and → showing cells in the mucous membrane layer; e. body of stomach, showing the overall structure of the stomach; f. cardiac stomach, showing the hierarchical structure of the cardia of the stomach and mucosal epithelium; g. body of stomach, ↑ showing gastric pit; → showing tunica mucosa; h. body of stomach, showing muscle layers; ↓ showing the outer thin serous, →← showing longitudinal muscle formed wavy wall wrinkling; i. pyloric stomach crosscutting showing the cavity wall structure as a whole; j. pyloric stomach partial enlargement, ← → showing pyloric stomach ring muscle layer thickness; k. rugae gastricae, pyloric stomach branch ruffle local amplification; l. ceca crosscutting, → showing blind sac wall was very thin, ← showing Lumen was full of creases图2 黑鱾食道、胃及幽门盲囊的组织学观察 |
Fig. 3 The intestinal and liver histology of Girella leonine. a. Before bowel cross-cutting, →← showing developed small intestinal villus cavity is separated into several independent parts, and lamina propria is extremely rich; b. After bowel cross-cutting, → showing cavity developed small intestinal villus, lack of branches; c. After bowel cross-cutting, → showing small intestinal villus in the cavity, lack of branches; d. before bowel cross-cutting locally developed, → showing branch of small intestinal villus, ↑ showing a large number of goblet cells; e. Im in cross-cutting locally developed,→ showing branch of small intestinal villus, ↑ showing a large number of goblet cells; f. after bowel cross-cutting locally developed,→ showing branch of small intestinal villus, ↑ showing a large number of goblet cells; g. intestinal villi, → showing the central lacteals was rich, and goblet cells mucous membrane layer was rich; h. hepatic lobule, → showing around the central vein to radiation around the liver cell line; i. lobular partial enlargement, → showing polygonal liver cells and the distribution of small artery图3 黑鱾肠道、肝脏的组织学观察 |
The authors have declared that no competing interests exist.
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