热带海洋学报 ›› 2022, Vol. 41 ›› Issue (5): 117-120.doi: 10.11978/2021168

• 海洋生物学 • 上一篇    下一篇

深海冷泉来源真菌Talaromyces helicus SCSIO41311中聚酮类化学成分研究

丛梦静1,3(), 胡怡伟1,3, 赵凯1, 张晓勇2, 刘永宏1,3, 王俊锋1,3()   

  1. 1.中国科学院南海海洋研究所, 热带海洋生物资源与生态重点实验室, 广东 广州 510301
    2.华南农业大学, 海洋学院, 广东 广州 510642
    3.中国科学院大学, 北京 100049
  • 收稿日期:2021-12-01 修回日期:2021-12-17 出版日期:2022-09-10 发布日期:2021-12-21
  • 通讯作者: 王俊锋
  • 作者简介:丛梦静(1999—), 女, 内蒙古自治区兴安盟人, 在读硕士, 研究方向为海洋药物化学。email: congmengjing@mails.ucas.ac.cn
  • 基金资助:
    海南省重大科技计划(ZDKJ202018);国家自然科学基金(41776169);广东省自然科学基金(2021A1515011523)

Study on the polyketides from a cold-seep-derived Talaromyces helicus SCSIO41311

CONG Mengjing1,3(), HU Yiwei1,3, ZHAO Kai1, ZHANG Xiaoyong2, LIU Yonghong1,3, WANG Junfeng1,3()   

  1. 1. CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
    2. College of Marine Science, South China Agricultural University, Guangzhou 510642, China
    3. University of the Chinese Academy of Sciences, Beijing 100049, China
  • Received:2021-12-01 Revised:2021-12-17 Online:2022-09-10 Published:2021-12-21
  • Contact: WANG Junfeng
  • Supported by:
    Finance Science and Technology Project of Hainan Province(ZDKJ202018);National Natural Science Foundation of China(41776169);Guangdong Basic and Applied Basic Research Foundation(2021A1515011523)

摘要:

文章旨在研究南海冷泉来源真菌Talaromyces helicus SCSIO41311中聚酮类次生代谢产物。主要采用正相硅胶柱层析、反相硅胶柱层析、Sephadex LH-20、高效液相色谱(high performance liquid chromatography, HPLC)及薄层色谱(thin layer chromatography, TLC)等多种方法进行分离纯化; 通过核磁共振波谱(nuclear magnetic resonance, NMR)、质谱(mass spectrometry, MS)等现代波谱分析方法及理化性质进行结构鉴定。从中分离得到7个聚酮类化合物, 分别为: desmethylsulochrin (1)、sulochrin (2)、monomethylsulochrin (3)、circinophoric acid (4)、dimethyl 2,3-dimethylosoate (5)、endocrocin (6)和1-methyl emodin (7)。所有化合物均为首次从深海冷泉微生物中得到, 其中化合物1为首次报道其核磁数据。

关键词: 深海, 冷泉, 聚酮类化合物, 结构鉴定

Abstract:

In this study, we aim to investigate the secondary metabolites of polyketides from the South China Sea cold-seep-derived Talaromyces helicus SCSIO41311. The polyketides were isolated by normal phase silica gel column chromatography, reverse phase silica gel column chromatography, Sephadex LH-20, high-performance liquid chromatography (HPLC) and thin layer chromatography (TLC). The structures of compounds were identified by nuclear magnetic resonance spectrum (NMR), mass spectrometry (MS) and literature analysis. Seven polyketides were obtained and elucidated as follows: desmethylsulochrin (1), sulochrin (2), monomethylsulochrin (3), circinophoric acid (4), dimethyl 2,3-dimethylosoate (5), endocrocin (6) and 1-methyl emodin (7). All compounds were obtained for the first time from deep sea cold-seep. NMR data of 1 was firstly reported.

Key words: deep sea, cold-seep, polyketides, structure identification

中图分类号: 

  • P735.4