基于形态学和高通量测序的春季南澳海域浮游植物群落特征及其与环境因子关系

doi:10.11978/2024046

热带海洋学报 ›› 2025, Vol. 44 ›› Issue (1): 53-65.doi: 10.11978/2024046CSTR: 32234.14.2024046

基于形态学和高通量测序的春季南澳海域浮游植物群落特征及其与环境因子关系

周志希¹(), 唐汇娟¹(), 柯志新²^,³, 刘甲星², 周伟华²

1.华南农业大学, 广东广州 510640
2.中国科学院热带海洋生物资源与生态重点实验室, 中国科学院南海海洋研究所, 广东广州 510301
3.粤东上升流区海洋生态系统综合观测研究站, 广东汕头 515041

收稿日期:2024-02-28 修回日期:2024-03-25 出版日期:2025-01-10 发布日期:2025-02-10
通讯作者: 唐汇娟
作者简介:
周志希(1999—), 男, 湖北省荆州市人, 硕士研究生, 主要从事海洋浮游植物生态学研究。email: 20223140078@stu.scau.edu.cn
基金资助:
广东省科技计划项目(2021B1212050023); 广东省基础与应用研究金(2022A1515010656); 广州市重点研发计划(2023B03J1328)

Phytoplankton community structure and its relationship with environmental factors in the spring coastal region of Nan’ao based on morphology and high-throughput sequencing

ZHOU Zhixi¹(), TANG Huijuan¹(), KE Zhixin²^,³, LIU Jiaxing², ZHOU Weihua²

1. South China Agricultural University, Guangzhou 510640, China
2. Key Laboratory of Tropical Marine Bio-resources and Ecology, Chinese Academy of Sciences, Guangzhou 510301, China
3. Comprehensive Observatory of Marine Ecosystems in the Upwelling Regions of Eastern Guangdong, Shantou 515041, China

Received:2024-02-28 Revised:2024-03-25 Online:2025-01-10 Published:2025-02-10
Contact: TANG Huijuan
Supported by:
Special Fund for Science and Technology Planning Project of Guangdong Province of China(2021B1212050023); Guangdong Basic and Applied Basic Research Foundation(2022A1515010656); Guangzhou Municipal Key Science and Technology Project(2023B03J1328)

摘要/Abstract

摘要：

利用形态学鉴定和高通量测序技术, 对2022年5月南澳海域浮游植物群落结构进行了研究, 探讨其空间分布与环境因子的关系, 并对两种不同方法得出的结果进行了比较和讨论。根据环境因子聚类分析, 调查海域可分为近岸区域和远岸区域。通过形态学方法鉴定浮游植物5门8纲27目39科52属105种, 高通量测序鉴定出6门32纲97目155科272属 543种, 其中硅藻门(Bacillariophyta)和甲藻门(Dinophyta)为优势类群, 分别平均占形态学鉴定结果的74.54%和24.78%以及高通量测序结果的 17.52%和67.84%。形态学鉴定结果显示浮游植物丰度变化范围为0.11×10⁵~6.85×10⁵cells·L^-1, 与叶绿素a浓度分布显著相关, 浮游植物多样性指数低于高通量测序方法。两种方法得到优势种均为7种, 其中形态学鉴定结果中绝对优势种为扁面角毛藻(Chaetoceros compressus), 高通量测序鉴定结果中绝对优势种为球形异帽藻(Heterocapsa rotundata)。相关性分析结果表明, 春季影响南澳浮游植物群落结构的主要环境因子是pH、盐度和溶解态无机磷。利用形态学鉴定与高通量测序技术相结合的方法, 可以更全面、更准确地描述浮游植物群落结构及多样性。

关键词: 南澳, 浮游植物, 群落结构, 高通量测序, 环境因子

Abstract:

The structure of phytoplankton community was investigated based on morphological identification and high-throughput sequencing in the Nan’ao waters in May 2022. The spatial distribution of phytoplankton in relation to environmental factors was explored and results from two different methods were also compared and discussed. According to the cluster analysis of environmental factors, the survey area can be divided into nearshore area and offshore area. Totally, 105 species belong to 52 genera, 39 families, 27 orders, 8 orders, 5 phyla were morphological identified, while 543 species in 6 phyla, 32 orders, 97 families, 155 families, 272 genera through high-throughput sequencing were identified. Bacillariophyta and Dinophyta were the main groups, composing 74.54% and 24.78% through microscopic identification, and 17.52% and 67.84% through high-throughput sequencing, respectively. The abundance of phytoplankton identified by morphology ranged from 0.11×10⁵ to 6.85×10⁵cells·L^-1, which was significantly correlated with the distribution of chlorophyll a concentration, and the phytoplankton diversity index was lower than that of the high-throughput sequencing method. 7 dominant species were obtained by both methods, of which the absolutely dominant specie in the morphological identification results was Chaetoceros compressus, and the absolute dominant specie in the high-throughput sequencing identification results was Heterocapsa rotundata. Correlation analysis showed that the main environmental factor affecting phytoplankton community structure in Nan’ao in spring was pH, salinity and DIP (dissolved inorganic phosphorus). Using the combination of morphological identification and high-throughput sequencing technology, the structure and diversity of the phytoplankton community can be described more comprehensively and accurately.

Key words: Nan’ao Island, phytoplankton, community structure, high-throughput sequencing, environmental factor

中图分类号:

P735

周志希, 唐汇娟, 柯志新, 刘甲星, 周伟华. 基于形态学和高通量测序的春季南澳海域浮游植物群落特征及其与环境因子关系[J]. 热带海洋学报, 2025, 44(1): 53-65.

ZHOU Zhixi, TANG Huijuan, KE Zhixin, LIU Jiaxing, ZHOU Weihua. Phytoplankton community structure and its relationship with environmental factors in the spring coastal region of Nan’ao based on morphology and high-throughput sequencing[J]. Journal of Tropical Oceanography, 2025, 44(1): 53-65.

图/表 10

图1

图2

表1

图3

图4

图5

表2

图6

图7

图8

参考文献 71

[1]	蔡德华, 陈振明, 唐书怿, 2020. 南澳岛周边海域海水质量近10年变化趋势浅析[J]. 环境影响评价, 42(2): 63-66.
	CAI DEHUA, CHEN ZHENMING, TANG SHUYI, 2020. Analysis on the change trend of sea water quality around Nan’ao Island in recent 10 years[J]. Environmental Impact Assessment, 42(2): 63-66 (in Chinese with English abstract).
[2]	陈丹婷, 柯志新, 谭烨辉, 等, 2020. 汕头南澳-东山海域营养盐季节分布特征及其对浮游植物生长的潜在性限制[J]. 生态科学, 39(4): 41-50.
	CHEN DANTING, KE ZHIXIN, TAN YEHUI, et al, 2020. Seasonal distribution of nutrients concentrations and the potential limitation for phytoplankton growth in the coastal region of Nan’ao-Dongshan[J]. Ecological Science, 39(4): 41-50 (in Chinese with English abstract).
[3]	陈菊芳, 齐雨藻, 徐宁, 等, 2005. 大亚湾拟菱形藻水华及其在生物群落中的生态地位[J]. 海洋学报, 27(1): 114-119.
	CHEN JUFANG, QI YUZAO, XU NING, et al, 2005. Pseudo-Nitzschia bloom and its ecological role in the biological community in the Daya Bay, the South China Sea[J]. Acta Oceanologica Sinica, 27(1): 114-119 (in Chinese with English abstract).
[4]	陈炜婷, 姜广甲, 邓伟, 等, 2023. 基于GIS的2001-2020年广东近岸海域赤潮时空分布[J]. 环境工程, 41(S2): 49-53.
	CHEN WEITING, JIANG GUANGJIA, DENG WEI, et al, 2023. Temporal and spatial distribution of harmful algal blooms in Guangdong province coastal area during 2001-2020 based on GIS[J]. Environmental Engineering, 41(S2): 49-53 (in Chinese with English abstract).
[5]	杜虹, 王亮根, 曹会彬, 等, 2011. 汕头港浮游植物组成特征及其与环境的关系[J]. 生态学杂志, 30(8): 1757-1765.
	DU HONG, WANG LIANGGEN, CAO HUIBIN, et al, 2011. Phytoplankton community composition and its relationships with the environment in Shantou Harbor of South China[J]. Chinese Journal of Ecology, 30(8): 1757-1765 (in Chinese with English abstract).
[6]	郭玉洁, 2003. 中国海藻志[M]. 北京: 科学出版社 (in Chinese).
[7]	黄长江, 董巧香, 郑磊, 1999. 1997年底中国东南沿海大规模赤潮原因生物的形态分类与生态学特征[J]. 海洋与湖沼, 30(6): 581-590.
	HUANG CHANGJIANG, DONG QIAOXIANG, ZHENG LEI, 1999. Taxonomic and ecological studies on a large scale Phaeocystis pouchetii bloom in the southeast coast of China during late 1997[J]. Oceanologia Et Limnologia Sinica, 30(6): 581-590 (in Chinese with English abstract).
[8]	黄圆, 岑竞仪, 梁芊艳, 等, 2024. 基于高通量测序技术的深圳湾真核浮游植物群落结构研究[J]. 热带海洋学报, 43(2): 21-33.
	HUANG YUAN, CEN JINGYI, LIANG QIANYAN, et al, 2024. Study on the community structure of eukaryotic phytoplankton in the Shenzhen Bay based on high-throughput sequencing technology[J]. Journal of Tropical Oceanography, 43(2): 21-33 (in Chinese with English abstract).
[9]	暨卫东, 黄尚高, 1990. 台湾海峡西部营养盐变化特征-Ⅲ. 水系混合及浮游植物摄取转移对磷酸盐含量变化影响的统计分析[J]. 海洋学报, 12(4): 447-454 (in Chinese).
[10]	雷光英, 杨宇峰, 李宵, 2010. 龙须菜对赤潮异弯藻和海洋原甲藻的生长抑制效应[J]. 海洋环境科学, 29(1): 27-31.
	LEI GUANGYING, YANG YUFENG, LI XIAO, 2010. Inhibitory effects of Gracilaria lemaneiformis on growth of Heterosigma akashiwo and Prorocentrum micans[J]. Marine Environmental Science, 29(1): 27-31 (in Chinese with English abstract).
[11]	李冀刚, 杨振雄, 葛颂, 等, 2023. 汕头韩江榕江河口近岸海域浮游植物群落结构与环境因子的关系[J]. 海洋湖沼通报, 45(2): 133-141.
	LI JIGANG, YANG ZHENXIONG, GE SONG, et al, 2023. Structural characterization of phytoplankton community in adjacent waters of Hanjiang Rongjiang Estuary, Shantou and determination of its relationship with environmental factors[J]. Transactions of Oceanology and Limnology, 45(2): 133-141 (in Chinese with English abstract).
[12]	黎素菊, 洪捷娴, 陈树鹏, 2022. 柘林湾养殖区氮、磷季节分布特征及富营养化评价[J]. 江西水产科技, (4): 45-47, 51 (in Chinese).
[13]	李欣, 孙军, 田伟, 等, 2012. 2009年夏季南海北部的浮游植物群落[J]. 海洋科学, 36(10): 33-39.
	LI XIN, SUN JUN, TIAN WEI, et al, 2012. Phytoplankton community in the northern area of South China Sea in summer of 2009[J]. Marine Sciences, 36(10): 33-39 (in Chinese with English abstract).
[14]	林更铭, 杨清良, 2021. 西太平洋浮游植物物种多样性[M]. 北京: 科学出版社.
	LIN GENGMING, YANG QINGLIANG, 2021. Species diversity of phytoplankton in the western Pacific[M]. Beijing: Science Press (in Chinese).
[15]	刘陈, 魏南, 王庆, 等, 2019. 广东汕头南澳岛近岸海域浮游植物群落结构与环境特征[J]. 应用与环境生物学报, 25(5): 1091-1098.
	LIU CHEN, WEI NAN, WANG QING, et al, 2019. Phytoplankton community and environmental characteristics in the coastal waters of Nanao Island, Shantou, Guangdong[J]. Chinese Journal of Applied Environmental Biology, 25(5): 1091-1098 (in Chinese with English abstract).
[16]	刘卫东, 宋伦, 吴景, 2017. 环境样本中微型和微微型浮游植物高通量测序的引物优化[J]. 生态学报, 37(12): 4208-4216.
	LIU WEIDONG, SONG LUN, WU JING, 2017. Optimization of high-throughput sequencing primers for nanophytoplankton and picophytoplankton in environmental samples[J]. Acta Ecologica Sinica, 37(12): 4208-4216 (in Chinese with English abstract).
[17]	刘旭平, 单忠才, 李媛芳, 等, 2023. 滤食性贝类选择性摄食行为研究现状[J]. 特种经济动植物, 26(5): 89-92, 107 (in Chinese).
[18]	马华栋, 王平, 温玉波, 等, 2021. 基于生态足迹分析的南澳岛旅游业可持续发展评估[J]. 海洋开发与管理, 38(8): 61-66.
	MA HUADONG, WANG PING, WEN YUBO, et al, 2021. Evaluation of sustainable development of Nan’ao Island tourism based on ecological footprint analysis[J]. Ocean Development and Management, 38(8): 61-66 (in Chinese with English abstract).
[19]	彭璇, 马胜伟, 陈海刚, 等, 2014. 夏季柘林湾-南澳岛海洋牧场营养盐的空间分布及其评价[J]. 南方水产科学, 10(6): 27-35.
	PENG XUAN, MA SHENGWEI, CHEN HAIGANG, et al, 2014. Spatial distribution and assessment of nutrients in marine ranching in Zhelin Bay-Nanao Island in summer[J]. South China Fisheries Science, 10(6): 27-35 (in Chinese with English abstract).
[20]	宋荪阳, 黄金臣, 岳强, 等, 2023. 唐山市海水养殖尾水监测及分析[J]. 河北渔业, (7): 23-29.
	SONG SUNYANG, HUANG JINCHEN, YUE QIANG, et al, 2023. Water quality monitoring of tail water of marine aquaculture in Tangshan[J]. Hebei Fisheries, (7): 23-29 (in Chinese with English abstract).
[21]	苏纪兰, 王卫, 1990. 南海域台湾暖流源地问题[J]. 东海海洋, 8(3): 1-9.
	SU JILAN, WANG WEI, 1990. On the sources of the Taiwan warm current from the South China Sea[J]. Donghai Marine Science, 8(3): 1-9 (in Chinese with English abstract).
[22]	孙军, 刘东艳, 2004. 多样性指数在海洋浮游植物研究中的应用[J]. 海洋学报, 26(1): 62-75.
	SUN JUN, LIU DONGYAN, 2004. The application of diversity indices in marine phytoplankton studies[J]. Acta Oceanologica Sinica, 26(1): 62-75 (in Chinese with English abstract).
[23]	孙军, 2007. 今生颗石藻的有机碳泵和碳酸盐反向泵[J]. 地球科学进展, 22(12): 1231-1239.
	SUN JUN, 2007. Organic carbon pump and carbonate counter pump of living Coccolithophorid[J]. Advances in Earth Science, 22(12): 1231-1239 (in Chinese with English abstract).
[24]	谭香, 夏小玲, 程晓莉, 等, 2011. 丹江口水库浮游植物群落时空动态及其多样性指数[J]. 环境科学, 32(10): 2875-2882.
	TAN XIANG, XIA XIAOLING, CHENG XIAOLI, et al, 2011. Temporal and spatial pattern of phytoplankton community and its biodiversity indices in the Danjiangkou reservoir[J]. Environmental Science, 32(10): 2875-2882 (in Chinese with English abstract).
[25]	唐涛, 蔡庆华, 刘建康, 2002. 河流生态系统健康及其评价[J]. 应用生态学报, 13(9): 1191-1194.
	TANG TAO, CAI QINGHUA, LIU JIANKANG, 2002. River ecosystem health and its assessment[J]. Chinese Journal of Applied Ecology, 13(9): 1191-1194 (in Chinese with English abstract).
[26]	王艳, 聂瑞, 李扬, 等, 2010. 广东沿海角毛藻(Chaetoceros)的种类多样性及其地理分布[J]. 海洋科学进展, 28(3): 342-352.
	WANG YAN, NIE RUI, LI YANG, et al, 2010. Species diversity and geographical distribution of Chaetoceros in Guangdong coastal waters[J]. Advances in Marine Science, 28(3): 342-352 (in Chinese with English abstract).
[27]	王雨, 林茂, 林更铭, 等, 2011. 闽粤近岸夏季上升流区浮游植物群落组成及其影响因素[J]. 应用生态学报, 22(2): 503-512.
	WANG YU, LIN MAO, LIN GENGMING, et al, 2011. Community composition of phytoplankton in Fujian-Guangdong coastal upwelling region in summer and related affecting factors[J]. Chinese Journal of Applied Ecology, 22(2): 503-512 (in Chinese with English abstract).
[28]	吴转璋, 朱超, 唐萍, 等, 2023. 巢湖湖区浮游植物群落与水质因子相关性分析[J]. 生物学杂志, 40(1): 79-84.
	WU ZHUANZHANG, ZHU CHAO, TANG PING, et al, 2023. Correlation analysis of phytoplankton community and water quality factors in Chaohu Lake[J]. Journal of biology, 40(1): 79-84 (in Chinese with English abstract).
[29]	中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员, 2007. GB/T12763. 6-2007海洋调查规范第6部分:海洋生物调查 [S]. 北京: 中国标准出版社: 6-37.
	General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China, Standardization Administration of the People’s Republic of China, 2007. GB/T12763. 6-2007 Specifications for oceanographic survey—Part 6: Marine biological survey[S]. Beijing: Standards Press of China: 6-37 (in Chinese).
[30]	中华人民共和国生态环境部,2020. 近岸海域环境监测规范(HJ 442. 3-2020)第三部分近岸海域水质监测[S]. 北京: 中国环境出版集团出版.
	Ministry of Ecology and Environment of the People’s Republic of China, 2020. Technical specification for offshore environmental monitoring Part 3 offshore seawater quality monitoring[S]. Beijing: China Environmental Science Press.
[31]	周凯, 黄长江, 姜胜, 等, 2002. 2000-2001年柘林湾浮游植物群落结构及数量变动的周年调查[J]. 生态学报, 22(5): 688-698.
	ZHOU KAI, HUANG CHANGJIANG, JIANG SHENG, et al, 2002. Annual dynamics of phytoplankton in Zhelin Bay: 2000-2001[J]. Acta Ecologica Sinica, 22(5): 688-698 (in Chinese with English abstract).
[32]	朱小山, 吴玲玲, 杨瑶, 等, 2005. 粤东柘林湾增养殖区氮磷的分布特征及其富营养化状态评价[J]. 海洋湖沼通报, (3): 16-22.
	ZHU XIAOSHAN, WU LINGLING, YANG YAO, et al, 2005. Distribution characteristics of a and Pin Zhelin Bay, one of mariculture zones of Guangdong Province, and assessment of its eutrophication[J]. Transactions of Oceanology and Limnology, (3): 16-22 (in Chinese with English abstract).
[33]	AKCAALAN R, OZBAYRAM E G, KALELI A, et al, 2023. Does environmental DNA reflect the actual phytoplankton diversity in the aquatic environment? Case study of marine mucilage in the Sea of Marmara[J]. Environmental Science and Pollution Research, 30(28): 72821-72831.
[34]	ADOLF J E, BACHVAROFF T R, DEEDS J R, et al, 2015. Ichthyotoxic Karlodinium veneficum (Ballantine) J Larsen in the Upper Swan River Estuary (Western Australia): Ecological conditions leading to a fish kill[J]. Harmful Algae, 48: 83-93.
[35]	BARTON A D, IRWIN A J, FINKEL Z V, et al, 2016. Anthropogenic climate change drives shift and shuffle in North Atlantic phytoplankton communities[J]. Proceedings of the National Academy of Sciences of the United States of America, 113(11): 2964-2969.
[36]	BAZIN P, JOUENNE F, FRIEDL T, et al, 2014. Phytoplankton diversity and community composition along the estuarine gradient of a temperate macrotidal ecosystem: combined morphological and molecular approaches[J]. PLoS One, 9(4): e94110.
[37]	BOSAK S, SARNO D, 2017. The planktonic diatom genus Chaetoceros Ehrenberg (Bacillariophyta) from the Adriatic Sea[J]. Phytotaxa, 314(1): 1-44.
[38]	CYRONAK T, SCHULZ K G, JOKIEL P L, 2016. The Omega myth: What really drives lower calcification rates in an acidifying ocean[J]. ICES Journal of Marine Science, 73(3): 558-562.
[39]	ESENKULOVA S, SUTHERLAND B J G, TABATA A, et al, 2020. Comparing metabarcoding and morphological approaches to identify phytoplankton taxa associated with harmful algal blooms[J]. FACETS, 5(1): 784-811.
[40]	FIELD C B, BEHRENFELD M J, RANDERSON J T, et al, 1998. Primary production of the biosphere: integrating terrestrial and oceanic components[J]. Science, 281(5374): 237-240.
[41]	GILBERT J A, FIELD D, HUANG YING, et al, 2008. Detection of large numbers of novel sequences in the metatranscriptomes of complex marine microbial communities[J]. PLoS One, 3(8): e3042.
[42]	GOBLER C J, 2020. Climate change and harmful algal blooms: insights and perspective[J]. Harmful Algae, 91: 101731.
[43]	HALLEGRAEFF G M, ANDERSON D M, BELIN C, et al, 2021. Perceived global increase in algal blooms is attributable to intensified monitoring and emerging bloom impacts[J]. Communications Earth & Environment, 2: 117.
[44]	HENDEY N I, 1977. The species diversity index of some in-shore diatom communities and its use in assessing the degree of pollution insult on parts of the North Coast of Cornwall[J]. Nova Hedwigia, 54(8): 355-378.
[45]	HU Y O O, KARLSON B, CHARVET S, et al, 2016. Diversity of pico- to mesoplankton along the 2000 km salinity gradient of the Baltic Sea[J]. Frontiers in Microbiology, 7: 679.
[46]	IRIGOIEN X, HUISMAN J, HARRIS R P, 2004. Global biodiversity patterns of marine phytoplankton and zooplankton[J]. Nature, 429(6994): 863-867.
[47]	JIANG ZHAOYU, WANG YOUSHAO, CHENG HAO, et al, 2015. Spatial variation of phytoplankton community structure in Daya Bay, China[J]. Ecotoxicology, 24(7-8): 1450-1458.
[48]	JOHNSON M D, CARPENTER R C, 2012. Ocean acidification and warming decrease calcification in the crustose coralline alga Hydrolithon onkodes and increase susceptibility to grazing[J]. Journal of Experimental Marine Biology and Ecology, 434-435: 94-101.
[49]	LEFEBVRE K A, ROBERTSON A, 2010. Domoic acid and human exposure risks: A review[J]. Toxicon, 56(2): 218-230.
[50]	LIN C H, LYUBCHICH V, GLIBERT P M, 2018. Time series models of decadal trends in the harmful algal species Karlodinium veneficum in Chesapeake Bay[J]. Harmful Algae, 73: 110-118.
[51]	LLANOS-RIVERA A, ÁLVAREZ-MUÑOZ K, ASTUYA-VILLALÓN A, et al, 2023. Sublethal effect of the toxic dinoflagellate Karlodinium veneficum on early life stages of zebrafish (Danio rerio)[J]. Environmental Science and Pollution Research, 30(10): 27113-27124.
[52]	LV JIN, WU HONGJUAN, CHEN MENGQIU, 2011. Effects of nitrogen and phosphorus on phytoplankton composition and biomass in 15 subtropical, urban shallow lakes in Wuhan, China[J]. Limnologica, 41(1): 48-56.
[53]	MA CAIHUA, YOU KUI, ZHANG MEIZHAO, et al, 2008. A preliminary study on the diversity of fish species and marine fish faunas of the South China Sea[J]. Journal of Ocean University of China, 7(2): 210-214.
[54]	MAUVISSEAU Q, HARPER L R, SANDER M, et al, 2022. The multiple states of environmental DNA and what is known about their persistence in aquatic environments[J]. Environmental Science & Technology, 56(9): 5322-5333.
[55]	MILLETTE N C, PIERSON J J, ACEVES A, et al, 2017. Mixotrophy in Heterocapsa rotundata: A mechanism for dominating the winter phytoplankton[J]. Limnology and Oceanography, 62(2): 836-845.
[56]	ODA T, SATO Y, KIM D, et al, 2001. Hemolytic activity of Heterocapsa circularisquama (Dinophyceae) and its possible involvement in shellfish toxicity[J]. Journal of Phycology, 37(4): 509-516.
[57]	PEDNEKAR S M, BATES S S, KERKAR V, et al, 2018. Environmental factors affecting the distribution of Pseudo-nitzschia in two monsoonal estuaries of Western India and effects of salinity on growth and domoic acid production by P. pungens[J]. Estuaries and Coasts, 41(5): 1448-1462.
[58]	REDFIELD A C, KETCHUM B H, RECHARDS F A, 1963. The influence of organisms on the composition of sea-water[M]//HILL M N, The Sea, Vol. 2. New York: Interscience: 26-77.
[59]	REYNOLDS C S, HUSZAR V, KRUK C, et al, 2002. Towards a functional classification of the freshwater phytoplankton[J]. Journal of Plankton Research, 24(5): 417-428.
[60]	SAMANTA B, BHADURY P, 2016. A comprehensive framework for functional diversity patterns of marine chromophytic phytoplankton using rbcL phylogeny[J]. Scientific Reports, 6: 20783.
[61]	SCHLÄPFER F, SCHMID B, 1999. Ecosystem effects of biodiversity: a classification of hypotheses and exploration of empirical results[J]. Ecological Applications, 9(3): 893-912.
[62]	SPRONG P A A, FOFONOVA V, WILTSHIRE K H, et al, 2020. Spatial dynamics of eukaryotic microbial communities in the German Bight[J]. Journal of Sea Research, 163: 101914.
[63]	TELESH I V, 2004. Plankton of the Baltic estuarine ecosystems with emphasis on Neva Estuary: a review of present knowledge and research perspectives[J]. Marine Pollution Bulletin, 49(3): 206-219.
[64]	TURNER R E, RABALAIS N N, JUSTIC' D, et al, 2003. Global patterns of dissolved N, P and Si in large rivers[J]. Biogeochemistry, 64(3): 297-317.
[65]	VANDEPUTTE D, KATHAGEN G, D’HOE K, et al, 2017. Quantitative microbiome profiling links gut community variation to microbial load[J]. Nature, 551(7681): 507-511.
[66]	WEIGAND H, BEERMANN A J, FEDOR Č, et al, 2019. DNA barcode reference libraries for the monitoring of aquatic biota in Europe: Gap-analysis and recommendations for future work[J]. Science of the Total Environment, 678: 499-524.
[67]	XIAO XI, SOGGE H, LAGESEN K, et al, 2014. Use of high throughput sequencing and light microscopy show contrasting results in a study of phytoplankton occurrence in a freshwater environment[J]. PLoS One, 9(8): e106510.
[68]	XU DANGHUI, FANG XIANGWEN, ZHANG RENYI, et al, 2015. Influences of nitrogen, phosphorus and silicon addition on plant productivity and species richness in an alpine meadow[J]. AoB PLANTS, 7: plv125.
[69]	XU XIN, YU ZHIMING, CHENG FANGJIN, et al, 2017. Molecular diversity and ecological characteristics of the eukaryotic phytoplankton community in the coastal waters of the Bohai Sea, China[J]. Harmful Algae, 61: 13-22.
[70]	YU LIUQIAN, GAN JIANPING, 2022. Reversing impact of phytoplankton phosphorus limitation on coastal hypoxia due to interacting changes in surface production and shoreward bottom oxygen influx[J]. Water Research, 212: 118094.
[71]	ZHU FEI, MASSANA R, NOT F, et al, 2005. Mapping of picoeucaryotes in marine ecosystems with quantitative PCR of the 18S rRNA gene[J]. FEMS Microbiology Ecology, 52(1): 79-92.

因子	温度/℃	盐度/‰	pH	浊度/NTU	DIN/(μmol·L^-1)	DIP/(μmol·L^-1)	DSi/(μmol·L^-1)	氮磷比
近岸	23.55±0.39	28.04±1.85	8.03±0.09	4.17±2.63	12.31±4.39	0.77±0.59	24.56±4.55	26.13±21.53
远岸	23.22±0.50	31.37±0.78	8.16±0.06	1.93±2.22	2.06±0.64	0.16±0.09	7.20±3.61	105.08±18.47
t-test	t=1.528, P=0.187	t=5.126, P<0.01	t=5.665, P<0.01	t=1.53, P=0.187	t=5.81, P<0.05	t=2.95, P<0.05	t=8.93, P<0.01	t=17.45, P<0.01

分类水平		优势度		出现频率%
门	种	形态学鉴定	高通量测序	出现频率%
硅藻门Bacillariophyta	扁面角毛藻Chaetoceros compressus	0.33	-	83.33
	微眼藻Minutocellus sp.	-	0.05	100
	多形微眼藻Minutocellus polymorphus	-	0.03	100
	艾氏角毛藻Chaetoceros eibenii	0.02	-	58.33
	洛氏菱形藻Nitzschia lorenziana	0.02	-	83.33
	尖刺拟菱形藻Pseudo-nitzschia pungens	0.02	-	100
	中肋骨条藻Skeletonema costatum	0.02	-	41.67
甲藻门 Dinophyta	球形异帽藻Heterocapsa rotundata	-	0.22	100
	海洋原甲藻Prorocentrum micans	0.13	-	100
	剧毒卡洛藻Karlodinium veneficum	-	0.09	100
	单眼藻Warnowia sp.	-	0.09	100
	单眼藻Warnowia spp.		0.07	100
	锥状斯氏藻Scrippsiella trochoidea	0.02	-	83.33
绿藻门Chlorophyta	共球藻目Picochlorum sp.	-	0.03	100

基于形态学和高通量测序的春季南澳海域浮游植物群落特征及其与环境因子关系

Phytoplankton community structure and its relationship with environmental factors in the spring coastal region of Nan’ao based on morphology and high-throughput sequencing

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 10

参考文献 71

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	柳原, 柯志新, 李开枝, 谭烨辉, 梁竣策, 周伟华. 人类活动和沿岸流影响下的粤东近海浮游动物群落特征[J]. 热带海洋学报, 2024, 43(4): 98-111.
[2]	刘玓玓, 张喜洋, 孙富林, 王明壮, 谭飞, 施祺, 王冠, 杨红强. 南海海滩岩微生物群落结构和特定菌株对其成因机制的启示*[J]. 热带海洋学报, 2024, 43(4): 112-122.
[3]	胡思敏, 周天成, 张琛, 刘胜, 李涛, 黄晖. 悬浮物对三亚珊瑚礁区浮游动物群落结构及其摄食的影响[J]. 热带海洋学报, 2024, 43(3): 122-130.
[4]	贾男, 周天成, 胡思敏, 张琛, 黄晖, 刘胜. 南沙群岛海域珊瑚礁区三种寄居蟹的摄食差异比较[J]. 热带海洋学报, 2024, 43(3): 109-121.
[5]	罗勇, 黄林韬, 杨剑辉, 练健生, 刘骋跃, 江雷, 梁宇娴, 陈伦举, 雷新明, 刘胜, 黄晖. 海南临高红牌—马袅沿岸海域造礁石珊瑚群落结构及其环境影响因子[J]. 热带海洋学报, 2024, 43(3): 72-86.
[6]	林先智, 周岩岩, 林皓晔, 胡思敏, 黄晖, 张黎, 刘胜. 南沙群岛珊瑚礁区黑斑鹦嘴鱼(Scarus globiceps)食性分析[J]. 热带海洋学报, 2024, 43(3): 100-108.
[7]	黄圆, 岑竞仪, 梁芊艳, 吕颂辉, 王建艳. 基于高通量测序技术的深圳湾真核浮游植物群落结构研究[J]. 热带海洋学报, 2024, 43(2): 21-33.
[8]	耿婉璐, 邢永泽, 张秋丰, 管卫兵. 广西北海红树林宜林滩涂大型底栖动物群落结构特征[J]. 热带海洋学报, 2024, 43(1): 107-115.
[9]	孙婷婷, 郝雯瑾, 徐鹏臻, 叶丽靖, 董志军. 海水酸化对海月水母螅状体共附生微生物的影响[J]. 热带海洋学报, 2023, 42(6): 111-119.
[10]	张兰兰, 程夏雯, 向荣, 邱卓雅, 常虎. 2019年春季孟加拉湾中部放射虫群落结构垂向变化^*[J]. 热带海洋学报, 2023, 42(4): 166-175.
[11]	宋星宇, 林雅君, 张良奎, 向晨晖, 黄亚东, 郑传阳. 粤港澳大湾区近海中小型浮游动物分布特征及影响因素^*[J]. 热带海洋学报, 2023, 42(3): 136-148.
[12]	赵红五一, 周雯, 曾凯, 邓霖, 廖健祖, 曹文熙. 基于浮游植物吸收系数和光合有效辐射的南海区域性分粒级初级生产力算法初探[J]. 热带海洋学报, 2023, 42(1): 43-55.
[13]	陈靖夫, 钟瑜, 王磊, 郭雨沛, 邱大俊. 环境DNA分析大亚湾夜光藻藻华对真核浮游生物群落的影响^*[J]. 热带海洋学报, 2022, 41(5): 121-132.
[14]	周雯, 魏盼盼, 李彩, 王桂芬, 郑文迪, 邓霖, 赵红五一, 余凌晖, 曹文熙. 琼东水体后向散射系数与浮游植物生物量的关系模型^*[J]. 热带海洋学报, 2022, 41(3): 29-37.
[15]	马文刚, 夏景全, 魏一凡, 尹洪洋, 覃乐政, 刘相波, 胡雪晴, 许强, 李秀保, 王爱民. 三亚蜈支洲岛海洋牧场近岛区底表大型底栖动物群落结构及评价[J]. 热带海洋学报, 2022, 41(3): 135-146.