检索
E-mail
RSS

作者投稿 主编审稿 专家审稿 远程编辑

热带海洋学报

• • 上一篇    

华南沿海潟湖反硝化微生物活性与群落特征分析

容清1，冼汉标2，董旭辉2，叶飞1，吴佳鹏1，王雨1，李佳慧3，洪义国1   

  1. 1.广州大学大湾区环境研究院，广东 广州 510007;

    2.广州大学地理科学与遥感学院，广东 广州 510006;

    3.陆军特种作战部队，广西 桂林 541000

  • 收稿日期:2025-02-25 修回日期:2025-04-03 接受日期:2025-04-08
  • 通讯作者: 王雨
  • 基金资助:
    国家自然科学基金项目（42476144）；广东省自然科学基金面上项目（2023A1515011747）

Denitrifying microbial activity and community characteristics in coastal lagoons of South China

RONG Qing1, XIAN Hanbiao2, DONG Xuhui2, YE Fei1, WU Jiapeng1, WANG Yu1, LI Jiahui3, HONG Yiguo1   

  1. 1， Institute of Environmental Research at Greater Bay Area, Guangzhou University, Guangzhou 510007, China;

    2. School of Geography and Remote Sensing, Guangzhou University, Guangzhou 510006, China;

    3. PLA Army Academy of Special Operations, Guilin 541000, China

  • Received:2025-02-25 Revised:2025-04-03 Accepted:2025-04-08
  • Supported by:

     National Natural Science Foundation of China (42476144); General Program of Guangdong Basic and Applied Basic Research Foundation (2023A1515011747)

PDF (PC)

赞

摘要： 本研究旨在探讨华南沿海潟湖中反硝化微生物的活性及其群落影响因素，以揭示潟湖生态系统中氮循环的关键机制。研究选取了华南地区的三个典型潟湖，通过测定水体和沉积物的理化参数，结合反硝化速率和宏基因组测序数据，分析了三种反硝化功能基因（nirK、nirS、nosZ）的群落组成及其与环境因子的关系。研究结果表明，华南潟湖的反硝化速率显著低于海洋沉积物平均水平，主要受限于沉积物中低营养盐浓度、较高的水体透明度和有限的水体交换能力。三种反硝化功能基因群落中nirK基因的丰度和多样性显著低于nirS和nosZ，表明nirK对环境变化更为敏感。不同潟湖中，nirK、nirS、nosZ的群落组成存在显著差异，目水平上的优势物种分别为Nitrosopumilales、Oceanospirillales和Flavobacteriales，主要群落组成与海洋环境中的反硝化微生物更加接近。水体透明度、pH值、NH₄⁺浓度是调控三种反硝化功能基因群落组成的关键环境因子。nirS群落更多受到沉积物理化参数影响，而nirK群落更多地受到水体理化参数影响。nirS和nosZ群落受相同环境因子的影响更多，表明它们在反硝化过程中具有更强的协同作用。本研究为认识潟湖生态系统的反硝化功能与微生物群落组成提供了数据支撑，并为潟湖的保护和管理提供了依据。

关键词: 潟湖, 反硝化, 群落特征, 水体透明度, 营养盐浓度

Abstract: The objective of this study was to investigate the activity of denitrifying microorganisms and the factors influencing their community composition in lagoons along the coast of South China, with the aim of elucidating the key mechanisms underlying the nitrogen cycle in lagoon ecosystems. Three representative lagoons in South China were selected for this research. By measuring the physicochemical parameters of water and sediment, combined with denitrification rates and metagenomic sequencing data, we analyzed the community composition of three denitrifying functional genes (nirK, nirS, nosZ) and their correlations with environmental factors. The results indicated that the denitrification rate in South China lagoons was significantly below the average for marine sediments, primarily due to low nutrient concentration, high water transparency, and limited sediment water exchange capacity. Notably, the abundance and diversity of nirK genes within the three denitrification functional gene communities were significantly lower than those of nirS and nosZ, suggesting that nirK is more susceptible to environmental variations. Significant differences were observed in the community composition of nirK, nirS, and nosZ among the different lagoons. At the order level, the dominant species were Nitrosopumilales, Oceanospirillales, and Flavobacteriales, respectively, with the main community composition closely resembling that of denitrifying microorganisms in the marine environment. Water transparency, pH, and NH4+ concentration emerged as the key environmental factors regulating the composition of the three denitrification functional gene communities. Specifically, the nirS community was more influenced by sediment physicochemical parameters, while the nirK community was more affected by water physicochemical parameters. Additionally, the nirS and nosZ communities were similarly impacted by the same environmental factors, indicating a stronger synergistic effect during the denitrification process. This study provides valuable data to enhance our understanding of the denitrification function and microbial community composition in lagoon ecosystems, thereby laying a foundation for the protection and management of these ecosystems.

Key words: Lagoon, Denitrification, Community characteristics, Water transparency, Nutrient concentration.