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热带海洋学报 ›› 2010, Vol. 29 ›› Issue (6): 51-56.doi: 10.11978/j.issn.1009-5470.2010.06.051cstr: 32234.14.j.issn.1009-5470.2010.06.051

• 海洋生物学 • 上一篇    下一篇

凡纳滨对虾生长性状的双列杂交分析

林红军1, 2, 沈琪1, 张吕平1, 胡超群1, 梁立清3   

  1. 1. 中国科学院南海海洋研究所中国科学院海洋生物资源可持续利用重点实验室, 广东省应用海洋生物重点实验室, 广东 广州 510301; 2. 衡水学院生命科学学院, 河北 衡水 053000; 3. 湛江市东方实业有限公司, 广东 湛江524008
  • 收稿日期:2009-11-15 修回日期:2010-06-04 出版日期:2010-12-15 发布日期:2010-12-15
  • 通讯作者: 沈琪。
  • 作者简介:林红军(1982—), 男, 河北省邯郸市人, 硕士研究生, 主要从事对虾遗传育种研究。E-mail: dongyangsea@163.com
  • 基金资助:

    国家“863”计划子课题(2006AA10A406, 2010AA10A401); 国家科技支撑计划子课题(2006BAD01A1303); 广东省海洋渔
    业科技推广专项(A200899A02)

A diallel analysis on growth traits in shrimp Litopenaeus vannamei

LIN Hong-jun1,2, SHEN Qi1, ZHANG Lü-ping1, HU Chao-qun1, LIANG Li-qing3   

  1. 1. CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, Key Laboratory of Marine Biological Application of Guangdong Province, South China Sea Institute of Oceanology, CAS, Guangzhou 510301, China; 2. Life Science College of Hengshui University, Hengshui 053000, China; 3. Zhanjiang City Dongfang Industrial Co, Ltd., Zhanjiang 524008, China
  • Received:2009-11-15 Revised:2010-06-04 Online:2010-12-15 Published:2010-12-15
  • Contact: 沈琪。
  • About author:林红军(1982—), 男, 河北省邯郸市人, 硕士研究生, 主要从事对虾遗传育种研究。E-mail: dongyangsea@163.com
  • Supported by:

    国家“863”计划子课题(2006AA10A406, 2010AA10A401); 国家科技支撑计划子课题(2006BAD01A1303); 广东省海洋渔
    业科技推广专项(A200899A02)

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被引次数

16 | 9

摘要：

以来源于两个不同遗传背景群体的凡纳滨对虾Litopenaeus vannamei为亲本, 设置PP(P♀×P♂)、     PH(P♀×H♂)、HP(H♀×P♂)、HH(H♀×H♂) 4个实验组(P代表进口群体, H代表选育群体), 进行进口个体(P♂和P♀)与选育个体(H♂和H♀)间的双列杂交实验, 分析比较其子一代的生长与存活情况。结果发现, 对虾存活由高到低顺序为HH>HP>PH>PP, PH实验组表现出明显的存活杂种优势, 其育苗期间的杂种优势平均值为(27.72?11.88)%, 下塘90d后成活率的杂种优势为30.50%。在生长前期(30日龄以前)对虾平均体长和体重的顺序是HP>PH>HH>PP, 而在生长后期则为PP>HP>PH>HH。HP实验组在整个养成期(10、30、60和90d)均表现出体长和体重上的杂种优势, 杂种优势值分别为24.00%、13.71%、2.55%、2.79%和112.46%、54.06%、8.16%、12.48%。结果表明, 选育群体为母本、进口群体为父本的对虾杂交后代的生长和存活性状明显提高。

关键词: 凡纳滨对虾, 双列杂交, 杂种优势, 生长, 成活率

Abstract:

The authors analyzed the growth and survival of the first generation of Litopenaeus vannamei after crossing within and between imported shrimp individuals (P♂, P♀) and selected individuals (H♂, H♀) using complete diallel crosses to set up four combinations, PP(P♀×P♂), PH(P♀×H♂), HP(H♀×P♂) and HH(H♀×H♂). The results showed that survival rate was in the order of HH>HP>PH>PP, and the survival heterosis appeared in PH, with average survival heterosis of (27.72?11.88)% in the young stage and 30.50 at the 90th day. In the early growth stage (the first 30 days), the average body length and weight were both sequenced as HP> PH> HH> PP, while in the late growth, as PP> HP> PH> HH. The growth heterosis was found in body length and weight. The values of body length heterosis were 24.00%, 13.71%, 2.55%, and 2.79%; while the values of body weight heterosis were 112.46%, 54.06%, 8.16%, and 12.48% at the 10th, 30th, 60th and 90th days. In conclusion, the growth and survival traits of hybrids offspring crossing selection individuals as male parents and imported individuals as female parents will be improved significantly.

Key words: Litopenaeus vannamei, diallel cross, heterosis, growth, survival rate