核酸指标对桡足类安氏伪镖水蚤摄食率、生长率及产卵率的指示作用

doi:10.11978/2017053

热带海洋学报 ›› 2018, Vol. 37 ›› Issue (2): 26-35.doi: 10.11978/2017053CSTR: 32234.14.2017053

核酸指标对桡足类安氏伪镖水蚤摄食率、生长率及产卵率的指示作用

何学佳¹(), 杨华明¹, 吕柏东², 尹健强³

1. 暨南大学赤潮与海洋生物学研究中心, 水体富营养化与赤潮防治广东普通高校重点实验室, 广东广州 510632
2. 广州工商学院, 广东广州333300
3. 中国科学院南海海洋研究所, 中国科学院热带海洋生物资源与生态重点实验室, 广东广州 510301

收稿日期:2017-05-12 修回日期:2017-06-26 出版日期:2018-04-10 发布日期:2018-04-11
作者简介:
作者简介：何学佳(1978—), 女, 贵州省遵义市人, 副研究员, 博士, 主要从事浮游动物生态化学计量学和营养生理生态方面的研究。E-mail: thexuejia@jnu.edu.cn
基金资助:
国家自然科学基金项目(41276153、41176087);国家基金委-广东联合重点基金(U1301235);中国科学院海洋生物资源可持续利用重点实验室开放基金项目(LMB111008)

Nucleic acid proxies for ingestion rate, growth and reproduction in copepod Pseudodiaptomus annandalei

Xuejia HE¹(), Huaming YANG¹, Baidong LYU², Jianqiang YIN³

1. Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Research Center of Harmful Algae and Marine Biology, Jinan University, Guangzhou 510632, China
2. Guangzhou College of Technology and Business, Guangzhou 333300, China
3. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China

Received:2017-05-12 Revised:2017-06-26 Online:2018-04-10 Published:2018-04-11
Supported by:
National Natural Science Foundation of China (41276153, 41176087);Joint Project of National Nature Science Foundation of China-Guangdong (U1301235);Open Fund Project of Key Laboratory of Sustainable Utilization of Marine Biological Resources of Chinese Academy of Sciences (LMB111008)

摘要/Abstract

摘要：

核酸指标可表征桡足类营养及生长状态, 然而多物种和多指标比较研究的相对缺乏却限制了现场海区调查中对核酸标志物的广泛应用。本研究测定了系列饵料浓度(0.2~2.0μg C·mL^-1)喂食的安氏伪镖水蚤Pseudodiaptomus annandalei的桡足幼体Ⅱ期个体的摄食率、生长率及成体产卵率, 并就生化组成, 包括RNA含量、DNA含量、蛋白含量(以下简写为PRO)、RNA:DNA、RNA:PRO、DNA:PRO及元素组成(N含量、P含量、C:N 和C:P比值)进行定量分析和计算。摄食率、生长率、产卵率及桡足幼体的RNA相关的核酸指标随饵料浓度的变化趋势均符合双曲线模型, 而雌性成体中仅RNA:DNA比值符合此模型。对RNA相关指标与各生理参数的相关性分析显示, 桡足幼体的RNA相关指标均与摄食率及生长率呈显著线性相关性, 显著程度的顺序为RNA:DNA>RNA:PRO>RNA含量; 雌性成体中RNA:DNA及RNA:PRO比值与产卵率显著相关, 前者的相关性远较后者显著。此结果表明, 在常用的现场浮游桡足类调查的标志比值(RNA:DNA比值或RNA含量)之外, RNA:PRO比值亦可作为指示桡足类生长状态的标志比值, 但是当指示体生长时, 桡足类必须处于同一生长阶段。RNA相关指标与饵料浓度之间稳固的双曲线相关性提示我们, RNA相关指标可帮助评估桡足类的饵料环境。

关键词: 核酸标志物, 桡足类, 摄食, 生长, 生殖

Abstract:

Nucleic acid indices can indicate nutrition status and growth of copepods; yet, lack of comparison among different phylogenetic groups and indices constrains application of nucleic acid proxies in field investigation. In this study, ingestion rate, growth rate of copepodid at Stage Ⅱ and egg production rate were studied in brackish copepod Pseudodiaptomus annandalei at different food levels (0.2~2.0 μg C·mL^-1). Biochemical indices including mass specific RNA content, DNA content, RNA:DNA, and RNA: protein ratios, as well as elemental composition (mass specific phosphorus content, nitrogen content, atomic C:P, and C:N ratios ), were also measured and described. Ingestion rate, growth rate, egg production rate, and RNA-related indices in copepodids increased with increasing food level hyperbolically, so did RNA:DNA ratio in female adults. Ingestion and growth rates were significantly correlated with RNA-related indices in copepodids in a linear manner and the strength of correlation ranked as RNA:DNA>RNA:PRO>RNA content. Egg production rate, however, was correlated with RNA:DNA and RNA:PRO ratios in female adults with high and moderate significance, respectively. Our results suggested that RNA:PRO ratio is suitable to be an indicator of growth and reproduction rate in copepod besides RNA:DNA ratio and RNA content, which have been applied in field investigation. However, RNA:DNA as proxy of somatic growth should be confined to the same-aged copepod. In addition, the finding of hyperbolic correlation between RNA-related indices and food concentration implied that they may help prediction of food environment for copepod, but may be confined to food levels at unsaturated phase of functional responses of variables.

Key words: nucleic acid indicators, copepod, ingestion, somatic growth, reproduction

何学佳, 杨华明, 吕柏东, 尹健强. 核酸指标对桡足类安氏伪镖水蚤摄食率、生长率及产卵率的指示作用[J]. 热带海洋学报, 2018, 37(2): 26-35.

Xuejia HE, Huaming YANG, Baidong LYU, Jianqiang YIN. Nucleic acid proxies for ingestion rate, growth and reproduction in copepod Pseudodiaptomus annandalei[J]. Journal of Tropical Oceanography, 2018, 37(2): 26-35.

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参考文献 20

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生长阶段	指标	y_max	b	c	r	p
桡足幼体	摄食率	0.053±0.006	2.04±0.62	0	0.95	0.012
	生长率	0.16±0.0053	3.3±0.34	0	0.99	0.001
	RNA:干重	0.083±0.0019	8.1±0.85	0	0.97	0.0054
	RNA:PRO	0.14±0.0008	11±0.37	0	0.99	<0.001
	RNA:DNA	15±3.3	0.56±0.38	1.1±0.56	0.96	<0.01
雌性成体	产卵率	6.8±0.34	2.6±0.38	0	0.99	<0.01
	RNA:DNA	15±0.26	2.6±0.82	0.56±0.20	0.97	<0.001
	C:P	434±15	7.7±1.2	0	0.95	0.015

生长阶段	生理指标	核酸指标和元素组成	Pearson分析		y=ax+b
生长阶段	生理指标	核酸指标和元素组成	p	r	a	b
桡足Ⅱ期	摄食率	RNA︰干重	0.018*	0.725	0.454	0.065
		RNA︰DNA	0.000**	0.953	114.8	6.956
		RNA︰PRO	0.003**	0.836	3.815	-0.029
		P含量/%	0.509	0.237	-0.443	0.320
		N含量/%	0.797	0.093	1.05	3.957
		C:P	0.510	0.237	155.1	103.5
		C:N	0.857	0.066	-1.461	8.327
桡足Ⅱ期	生长率	RNA︰干重	0.005**	0.688	0.165	0.074
		RNA︰DNA	0.000**	0.814	39.60	4.942
		RNA︰PRO	0.004**	0.702	1.118	-0.064
		P含量/%	0.419	0.225	0.158	0.281
		N含量/%	0.307	0.238	1.212	3.812
		C:P	0.503	0.187	-48.443	116.13
		C:N	0.528	0.178	-1.515	8.514
雌性成体	产卵率	RNA︰干重	0.076	0.636	0.039	-0.001
		RNA︰DNA	0.000**	0.851	0.511	11.71
		RNA︰PRO	0.027*	0.565	3.815	-0.029
		P含量/%	0.000**	0.891	-0.019	0.303
		N含量/%	0.193	0.356	0.244	2.593
		C:P	0.000**	0.889	12.29	88.81
		C:N	0.132	0.408	-0.395	11.79

核酸指标对桡足类安氏伪镖水蚤摄食率、生长率及产卵率的指示作用

Nucleic acid proxies for ingestion rate, growth and reproduction in copepod Pseudodiaptomus annandalei

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