硫元素对小球藻异养生产虾青素的影响

doi:10.11978/j.issn.1009-5470.2011.01.101

热带海洋学报 ›› 2011, Vol. 30 ›› Issue (1): 101-106.doi: 10.11978/j.issn.1009-5470.2011.01.101cstr: 32234.14.j.issn.1009-5470.2011.01.101

硫元素对小球藻异养生产虾青素的影响

刘纪化1,2, 向文洲1 ,陈涛1, 左云龙1,2, 何慧1, 苏娇娇1,2, 肖伟1, 刘学东1

1. 中国科学院南海海洋研究所, 广东广州 510301; 2. 中国科学院研究生院, 北京 100039

收稿日期:2009-04-29 修回日期:2009-05-31 出版日期:2011-03-17 发布日期:2011-03-16
通讯作者: 刘学东。E-mail: xxxdddlll@yahoo.com.cn
作者简介:刘纪化(1982—), 男, 山东省邹平县人, 硕士, 从事微藻生物技术研究。E-mail: liujihua1982@gmail.com
基金资助:
广东省科技计划项目(2007B030300004); 广东省海洋与渔业局科技专项项目(A2008009-08)

Effect of sulphur on the growth and production of astaxanthin by Chlorella zofingiensis in heterotrophic culture

LIU Ji-hua1,2, XIANG Wen-zhou1, CHEN Tao1, ZUO Yun-long1,2, HE Hui1, SU Jiao-jiao1,2, XIAO Wei1, LIU Xue-dong1

1. South China Sea Institute of Oceanology, CAS, Guangzhou 510301, China; 2. Graduate University of CAS, Beijing 100039, China

Received:2009-04-29 Revised:2009-05-31 Online:2011-03-17 Published:2011-03-16
Contact: 刘学东。E-mail: xxxdddlll@yahoo.com.cn
About author:刘纪化(1982—), 男, 山东省邹平县人, 硕士, 从事微藻生物技术研究。E-mail: liujihua1982@gmail.com
Supported by:
广东省科技计划项目(2007B030300004); 广东省海洋与渔业局科技专项项目(A2008009-08)

摘要/Abstract

摘要：

研究了不同浓度硫元素对小球藻Chlorella zofingiensis异养生长和合成虾青素的影响。结果表明, 低硫条件下, 细胞分裂受到严重抑制, 虾青素迅速积累且含量显著提高, 但限制藻细胞干重的增加。硫元素3μmol•L?1浓度下, 第14天虾青素含量最大, 达到1.19mg•g?1, 高出300μmol•L?1硫元素组40.68%。硫元素3000μmol•L?1浓度下, 在第14天时获得最大虾青素产量, 达到9.99mg•L?1。与300μmol•L?1硫元素组相比, 3000μmol•L?1硫元素组获得了更高的生物量和虾青素产量。而前者在培养后期, 出现了一定程度的蛋白质抑制现象。本研究认为, 在单批异养培养中, 硫元素300μmol•L?1可能是葡萄糖代谢获得最大生物量所需的最低浓度, 因此在流加葡萄糖培养的工业化生产中, 一次性加入更高浓度的硫元素, 可望获得更高的虾青素产量。

关键词: 小球藻, 硫元素, 生长, 虾青素, 异养培养

Abstract:

The effect of various sulphur concentrations on the growth of Chlorella zofingiensis and the astaxanthin accumula-tion in its heterotrophic culture were investigated. The results indicated that low sulphur concentration favoured rapid and significant astaxanthin accumulation while the biomass enhancement and cell division were inhibited severely. The addition of 3μmol•L?1 sulphur to the culture led to a maximum astaxanthin content of 1.19mg•g?1, increased by 40.68% compared with the 300μmol•L?1 sulphur cultures. The highest yield of astaxanthin was 9.99mg•L?1, which was obtained in the medium containing 3000μmol•L?1 sulphur on Day 14. The addition of 3000μmol•L?1 sulphur to the culture enhanced the biomass and yield of an-taxanthin compared with the 300μmol•L?1 sulphur cultures in which the protein content decreased in the later stage. To attain the maximum biomass in heterotrophic culture, the 300μmol•L?1 sulphur concentration could be the minimum limit for glucose utilization in batch culture. The results indicated that the productivity of the antaxanthin and biomass could be enhanced by application of higher concentration of sulphur in the industrial production through glucose fed-batch fermentation.

刘纪化,向文洲,陈涛,左云龙,何慧,苏娇娇,肖伟,刘学东. 硫元素对小球藻异养生产虾青素的影响[J]. 热带海洋学报, 2011, 30(1): 101-106.

LIU Ji-hua,XIANG Wen-zhou,CHEN Tao,ZUO Yun-long,HE Hui,SU Jiao-jia. Effect of sulphur on the growth and production of astaxanthin by Chlorella zofingiensis in heterotrophic culture[J]. Journal of Tropical Oceanography, 2011, 30(1): 101-106.

参考文献

[1] 李浩明, 高蓝. 虾青素的结构、功能与应用[J]. 精细化工, 2003, 20(1): 32-37.

[2] IP P F, WONG K H, CHEN F. Enhancement production of astaxanthin by the green microalga Chlorella zofingiensis in mixotrophic culture[J]. Process Biochemistry, 2004, 39(11): 1761-1766.

[3] HIGUERA-CIAPARA I, FELIX-VALENZUELA L, GOYCOOLEA F M. Astaxanthin: A review of its chemistry and applications[J]. Critical Reviews in Food Science and Nutrition, 2006, 46(12): 185-196.

[4] LORENZ R T, CYSEWSKI G R. Commercial potential for Haematococcus microalgae as a natural source of astaxanthin[J]. Trends Biotechnol, 2000, 18 (4): 160-167.

[5] IP P F, CHEN F. Production of astaxanthin by the green microalga Chlorella zofingiensis in the dark[J]. Process Biochemistry, 2005, 40(2): 733-738.

[6] 孙妮, 向文州, 何慧, 等. 碳氮比和光强对小球藻合成虾青素的影响[J]. 微生物学通报, 2008, 35(3): 353-357.

[7] 陈涛, 向文州, 何慧, 等. 不同碳源对小球藻Chlorella zofingiensis 异养生产虾青素的影响[J]. 微生物学通报, 2007, 34(5): 27-30.

[8] 张龙翔, 张庭芳, 李令媛. 生化实验方法和技术[M]. 北京: 高等教育出版社, 1997: 137-140.

[9] MILLER G L．Use of dinitrosalicylic acid reagent for determination of reducing sugar[J]. Anal Chem, 1959, 31(3): 426-428.

[10] BAROLI I, DO A D, YAMANE T, et al. Zeaxanthin accumulation in the absence of a functional xanthophylls cycle protects Chlamydomonas reinhardtii from photooxidative stress[J]. Plant Cell, 2003, 15 (4): 992-1008.

[11] RÜDIGER H, THOMAS L. Sulfur metabolism in plants and algae -a case study for an integrative scientific approach[J]. Photosynthesis Research, 2005, 86 (3): 297-298.

[12] NIKIFOROVA V J, KOPKA J, TOLSTIKOV V, et al. Systems rebalancing of metabolism in response to sulfur deprivation, as revealed by metabolome analysis of arabidopsis plants[J]. Plant Physiology, 2005, 138: 304-318.

[13] LEWIN R A. Physiology and biochemistry of algae[M]. Academic Press, New York, London, 1962: 619-622.

[14] 周云龙. 植物生物学[M]. 2版. 北京: 高等教育出版社, 1999: 160.

[15] DEL CAMPO J A, RODRIGUEZ H, MORENO J, et al. Accumulation of astaxanthin and lutein in Chlorella zofingiensis (Chlorophyta)[J].Appl Microbiol Biotechnol, 2004, 64(6): 848-854.

[16] SUN NI, WANG YAN, LI YAN-TAO, et al. Sugar-based growth, astaxanthin accumulation and carotenogenic transcription of heterotrophic Chorella zofingiensis (Chlorophyta)[J]. Process Biochemistry, 2008, 43(11): 1288-1292.

[17] WEI XIONG, LI XIU-FENG, XIANG JIN-YU, et al. High-density fermentation of microalga Chlorella protothecoides in bioreactor for microbio-diesel production[J]. Appl Microbiol Biotechnol, 2008, 78: 29-36.

硫元素对小球藻异养生产虾青素的影响

Effect of sulphur on the growth and production of astaxanthin by Chlorella zofingiensis in heterotrophic culture

PDF (PC)

赞

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	周伟华, 李颖心, 郭亚娟, 霍嘉欣, 宋严, 朱晴, 袁翔城, 刘胜, 黄晖. 海水酸化和升温对两种造礁石珊瑚生长和钙化的影响*[J]. 热带海洋学报, 2024, 43(3): 49-57.
[2]	杨芳芳, 肖志梁, 韦章良, 黄怡, 龙丽娟. 海水酸化与升温对孔石藻(Porolithon cf. onkodes)生长钙化的影响[J]. 热带海洋学报, 2023, 42(1): 87-97.
[3]	刘双双, 杨升, 刘星, 陈秋夏, 王金旺, 郭晋敏, 王佳玉, 王文卿, 吴伟志, 梁立成, 张小伟. 不同鲜重秋茄胚轴形态及其对幼苗生长的影响[J]. 热带海洋学报, 2022, 41(6): 56-66.
[4]	吴伟志, 赵志霞, 杨升, 梁立成, 陈秋夏, 卢翔, 刘星, 张小伟. 浙江省红树林分布和造林成效分析[J]. 热带海洋学报, 2022, 41(6): 67-74.
[5]	刘小菊, 施祺, 陶士臣, 杨红强, 张喜洋, 周胜男. 近165年来中沙环礁中北暗沙滨珊瑚生长率及其对海温变化的响应[J]. 热带海洋学报, 2022, 41(5): 64-73.
[6]	许瀚之, 张华, 熊盼盼, 何毛贤. 马氏珠母贝异速生长个体对免疫刺激的差异响应[J]. 热带海洋学报, 2022, 41(5): 180-188.
[7]	陈启明, 刘松林, 张弛, 崔黎军, 江志坚, 吴云超, 黄小平. 海南典型热带海草床4种代表性鱼类的生长特征及其对海草资源量变化的响应[J]. 热带海洋学报, 2020, 39(5): 62-70.
[8]	刘玉, 王雪辉, 杜飞雁, 刘必林, 张鹏, 刘梦娜, 邱永松. 基于耳石微结构的南海鸢乌贼日龄和生长研究[J]. 热带海洋学报, 2019, 38(6): 62-73.
[9]	杨海燕, 李洁琼, 刘红全, 黄小燕, 许钟涛, 罗远康, 禤金彩, 龙寒. 小球藻EC04产胞内多糖条件优化及抗氧化活性研究[J]. 热带海洋学报, 2019, 38(6): 98-104.
[10]	艾加林, 栗志民, 刘建勇, 申玉春. 九孔鲍MSTN基因cSNP多态性及与生长性状关联分析[J]. 热带海洋学报, 2019, 38(2): 78-85.
[11]	胡敏航, 陈天然, 张文静. 滨珊瑚Sr/Ca的种间和种内差异性^*[J]. 热带海洋学报, 2018, 37(6): 74-84.
[12]	何学佳, 杨华明, 吕柏东, 尹健强. 核酸指标对桡足类安氏伪镖水蚤摄食率、生长率及产卵率的指示作用[J]. 热带海洋学报, 2018, 37(2): 26-35.
[13]	郭亚娟, 周伟华, 袁翔城, 廖健祖, 江雷, 黄晖. 两种造礁石珊瑚对海水酸化和溶解有机碳加富的响应^*[J]. 热带海洋学报, 2018, 37(1): 57-63.
[14]	刘甲星, 周林滨, 柯志新, 李刚, 史荣君, 谭烨辉. 铝对海洋固氮蓝藻Crocosphaera watsonii生长及固氮速率的影响[J]. 热带海洋学报, 2017, 36(2): 12-18.
[15]	韦国建, 李耀国, 林坚士, 何毛贤. 马氏珠母贝生长性状QTL鉴定及基因型选择效应分析^*[J]. 热带海洋学报, 2017, 36(2): 19-25.