Journal of Tropical Oceanography ›› 2023, Vol. 42 ›› Issue (1): 145-151.doi: 10.11978/2022023CSTR: 32234.14.2022023

• Exploitation of Marine Resources • Previous Articles     Next Articles

Anti-quorum sensing active substances from a marine-derived actinobacterium Nocardiopsis dassonvillei JS106

MIAO Li(), QIAN Jiaxing, MO Jie, ZHOU Heng, QIAN Shenghui, DONG Kunming()   

  1. School of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, China
  • Received:2022-02-13 Revised:2022-04-23 Online:2023-01-10 Published:2022-04-28
  • Contact: DONG Kunming. email: kmdong@yzu.edu.cn
  • Supported by:
    Open Project of Key Laboratory of Marine Medicine, Guangdong Province and Key Laboratory of Tropical Marine Bio-resources and Ecology, Chinese Academy of Sciences(2018011008)

Abstract:

Quorum sensing inhibitors (QSIs) can regulate the pathogenicity by mediating the bacterial quorum sensing system, and it is not easy to cause resistance mutations. To deal with the bacterial drug resistance, QSIs might be useful in treating or cooperated treating bacterial infections. In this study, the anti-quorum sensing substances from a marine derived actinobacterium Nocardiopsis dassonvillei JS106 was investigated. Six compounds, 1, 6-dihydroxyphenazine (1), 6-hydroxy-1-methoxyphenazine (2), 4', 7-dimethoxyisoflavone (3), N-(2-hydroxyphenyl) acetamide (4), 1-methoxyphenazine (5) and 2-aminophenoxazin-3-one (6), were isolated from the spent culture medium of JS106 using VLC and HPLC. All of the pure compounds showed significant anti-quorum sensing activity that inhibited the violacein production of Chromobacterium violaceum ATCC 12472 without visible growth inhibition at the test concentrations. N. dassonvillei JS106 can produce many phenazines and has higher potential in anti-quorum sensing application.

Keywords marine actinomycetes; bioactivity; anti-quorum sensing activity; Nocardiopsis; phenazines