南海热带岛礁生物土壤结皮中细菌的分离及其固砂特性初步研究

doi:10.11978/2022266

Abstract

Abstract:

There are many microbial resources present in biological soil crusts of tropical reefs in the South China Sea, and the extracellular polysaccharides secreted by these microorganisms play an important role in promoting sand consolidation. In this study, three media, TSA, modified TSA and MA, were used to isolate and purify culturable bacteria from biological soil crusts in the Yongshu Reef and Luhuitou, Sanya, South China Sea. A total of 70 bacterial strains were isolated and purified in this study. And based on 16S rRNA gene identification, these isolated strains belonged to 3 phyla, 5 classes, 12 orders and 19 families, 25 genera. The dominant phylum was Firmicutes and the dominant genus was Bacillus. In addition, 22 strains had less than 98.65%16S rRNA gene similarities with known species, which were potential novel species. The extracellular polysaccharide content of purified strains was measured by ethanol precipitation method and phenol sulfate method, and the contents of 19 strains were more than 0.013 mg·mL^-1. Finally, 9 strains with the highest extracellular polysaccharide content were selected to determine the soil agglomeration ability. The results showed that strain SCSIO 17111 (Lysobacter) was the only strain which was able to maintain the stability of the biological soil crust clods by spraying. Our study provides high extracellular polysaccharide-producing strain resources for island reef management.

Key words: South China Sea reefs, culturable bacterial diversity, extracellular polysaccharide, sand fixation

HUANG Yu, WANG Lin, MAI Zhimao, LI Jie, ZHANG Si. Isolation and characterization of sand fixation ability of bacteria in biological soil crusts of the tropical islands, South China Sea[J].Journal of Tropical Oceanography, 2023, 42(6): 101-110.

Figures/Tables 9

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菌株编号	最近缘物种	16S rRNA基因相似性/%
SCSIO 17100	Microbacterium arborescens	98.55
SCSIO 17105	Microbacterium arborescens	98.55
SCSIO 17035	Microbacterium saccharophilum	98.48
SCSIO 17034	Bacillus zhanjiangensis	98.36
SCSIO 17007	Microbacterium pumilum	98.33
SCSIO 17026	Bacillus zhanjiangensis	98.29
SCSIO 17060	Devosia lucknowensis	98.28
SCSIO 17011	Microbacterium saccharophilum	98.26
SCSIO 17054	Bacillus oryzaecorticis	98.24
SCSIO 17069	Bacillus oryzaecorticis	98.23
SCSIO 17076	Microbacterium invictum	98.18
SCSIO 17016	Bacillus zhanjiangensis	98.15
SCSIO 17014	Skermanella aerolata	97.98
SCSIO 17004	Aquipuribacter nitratireducens	97.47
SCSIO 17099	Bacillus cohnii	97.21
SCSIO 17017	Bacillus salitolerans	97.02
SCSIO 17048	Nocardioides zeicaulis	96.94
SCSIO 17036	Mesorhizobium huakuii	96.93
SCSIO 17031	Bacillus herbersteinensis	96.85
SCSIO 17027	Devosia lucknowensis	96.66
SCSIO 17010	Bacillus abyssalis	96.56
SCSIO 17040	Kineosporia rhamnosa	95.90

菌株编号	产量/(mg·mL^-1)
SCSIO 17048	6.4533
SCSIO 17023	6.4067
SCSIO 17046	6.2300
SCSIO 17003	6.1800
SCSIO 17036	6.0767
SCSIO 17037	6.0467
SCSIO 17054	6.0200
SCSIO 17111	5.9833
SCSIO 17100	5.9733
SCSIO 17096	5.9633
SCSIO 17049	5.8067
SCSIO 17060	5.6267
SCSIO 17061	5.6233
SCSIO 17019	5.5200
SCSIO 17051	5.4067
SCSIO 17076	5.4033
SCSIO 17099	5.1933
SCSIO 17097 SCSIO 17010	5.0767 5.0267

菌株编号	产量/(mg·mL^-1)	菌株来源	菌株属
SCSIO 17111	0.0754	永暑礁结皮底层砂	Lysobacter
SCSIO 17003	0.0539	永暑礁结皮层	Bacillus
SCSIO 17096	0.0403	三亚结皮层	Agromyces
SCSIO 17051	0.0307	三亚结皮层	Bacillus
SCSIO 17100	0.0305	三亚结皮层	Microbacterium
SCSIO 17049	0.0252	三亚结皮层	Bacillus
SCSIO 17010	0.0213	永暑礁结皮层	Bacillus
SCSIO 17061	0.0209	三亚结皮层	Pseudoxanthomonas
SCSIO 17048	0.0186	三亚结皮层	Nocardioides
SCSIO 17060	0.0185	三亚结皮层	Devosia
SCSIO 17097	0.0182	三亚结皮层	Brachybacterium
SCSIO 17023	0.0180	永暑礁结皮底层砂	Bacillus
SCSIO 17046	0.0170	三亚结皮层	Halobacillus
SCSIO 17076	0.0168	永暑礁结皮层	Microbacterium
SCSIO 17099	0.0165	永暑礁结皮底层砂	Bacillus
SCSIO 17036	0.0161	三亚结皮层	Mesorhizobium
SCSIO 17037	0.0158	三亚结皮层	Ornithinimicrobium
SCSIO 17054	0.0151	三亚结皮层	Bacillus
SCSIO 17019	0.0137	永暑礁结皮底层砂	Pseudomonas

菌株编号	土壤团聚体厚度/mm	干筛结果/g (粒径>1.25mm)	菌株来源	菌株属
SCSIO 17111	6.00±0.13	13.56	永暑礁结皮底层砂	Lysobacter
SCSIO 17023	3.89±0.14	0	永暑礁结皮底层砂	Bacillus
SCSIO 17096	4.44±0.14	0	三亚结皮层	Agromyces
SCSIO 17049	3.67±0.07	0	三亚结皮层	Bacillus
SCSIO 17051	3.78±0.10	0	三亚结皮层	Bacillus
SCSIO 17003	3.33±0.13	0	永暑礁结皮层	Bacillus
SCSIO 17061	3.78±0.07	0	三亚结皮层	Pseudoxanthomonas
SCSIO 17100	2.00±0.19	0	三亚结皮层	Microbacterium
SCSIO 17010	2.11±0.20	0	永暑礁结皮层	Bacillus

Isolation and characterization of sand fixation ability of bacteria in biological soil crusts of the tropical islands, South China Sea

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