海水酸化对海月水母螅状体共附生微生物的影响

doi:10.11978/2023011

Abstract

Abstract:

Ocean acidification (OA) poses strong threat to marine organisms, and studying the impact of OA on their associated microbial communities is important for understanding the adaptive mechanisms of host and microbial response to environmental stresses. Moon jellyfish (Aurelia coerulea) is the dominant species of disaster jellyfish in China and plays an important role in marine ecosystem. In this study, we investigated the effects of future OA (pH 7.8 and 7.6) on the structure and function of the A. coerulea polyp-associated microbial communities using Biolog-ECO and metagenomic approaches. The results showed that the structures and functions of the microbial community associated with A. coerulea polyps were conserved in different degrees of seawater acidification. Biolog-ECO results showed that seawater acidification had little effect on the microbial metabolic activity, diversity index and carbon source utilization of the associated microbial community of A. coerulea polyps. The metagenomic results showed that seawater acidification did not affect the dominant species and community structure of the microorganisms. Tenericute, Proteobacteria, Firmicutes and Bacteroidetes were the dominant groups of the community. Moreover, there was no significant difference in the function of associated microorganisms exposed to different acidification conditions. Therefore, we speculate that future OA will not affect the polyps by altering the community structures of their associated microorganisms.

Key words: climate change, Biolog-ECO method, metagenomics, structure of community, metabolic function

SUN Tingting, HAO Wenjin, XU Pengzhen, YE Lijing, DONG Zhijun. Effects of seawater acidification on microorganisms associated with Aurelia coerulea polyps[J].Journal of Tropical Oceanography, 2023, 42(6): 111-119.

Figures/Tables 6

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References 34

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处理组	pH	温度/℃	盐度/‰	TA/(μmol·kg^-1)	pCO_2/μatm	DIC/(μmol·kg^-1)
对照	8.14±0.02	21.0±0.0	28.6±0.9	2521.53±34.21	529.67±27.56	2307.44±29.02
中度酸化	7.80±0.00	21.0±0.0	28.6±0.8	2523.60±36.20	1252.19±15.65	2447.38±33.77
重度酸化	7.60±0.01	21.0±0.0	28.5±0.8	2494.88±31.88	2028.32±28.00	2485.80±30.12

测量指标		F	P
AWCD	144h	F_2,6=2.287	P=0.183
多样性指数	Shannon's指数(H')	F_2,6=4.008	P=0.078
	Pielou指数(J)	F_2,6=4.008	P=0.078
	Simpson指数(D)	F_2,6=4.426	P=0.066
	Mclntosh指数(U)	F_2,6=0.794	P=0.495
碳源	糖	F_2,6=4.356	P=0.068
	氨基酸	F_2,6=0.839	P=0.477
	酯	F_2,6=0.713	P=0.528
	醇	F_2,6=1.828	P=0.240
	胺	F_2,6=0.670	P=0.546
	酸	F_2,6=0.837	P=0.478

Effects of seawater acidification on microorganisms associated with Aurelia coerulea polyps

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