河口岸带氨氮原位快速分析方法研究*

doi:10.11978/2023144

Abstract

Abstract:

With the goal of in situ reliable measuring of the ammonia nitrogen by a self-developed in situ analyzer, a rapid analysis method for ammonia nitrogen concentration in estuarine zones has been established based on the improved indophenol blue spectrophotometry. Through the orthogonal experimental analysis on the incomplete chromogenic reaction time and salinity, the optimal incomplete chromogenic reaction time for in situ automated rapid measurements under different salinity conditions has been established and the variation of the optimal incomplete chromogenic reaction time of the samples with salinity in each range has been determined. The study shows that the optimal reaction time for the rapid measurement of ammonia nitrogen in the range of salinity from 0‰ to 25‰ gradually increases with the increase of salinity and the minimum reaction time is 60s (salinity: 5‰) and the maximum reaction time is 180s (salinity: 25‰). With a 10 cm LWCC (liquid waveguide capillary cell) and 60s reaction time, the detection limit of this method acquired by taking 11 parallel samples of blank samples is 0.9945 μmol·L^-1 (salinity: 5‰), and the R² of the standard working curve of the method was more than 0.99, which proved that the improved indophenol blue spectrophotometric method can meet the in situ rapid analysis of ammonia nitrogen in estuarine zones. The in situ analyzer has deployed in the Zhanjiang Bay for the time series monitoring of ammonia nitrogen concentration, and the monitoring data revealed that there is a good negative correlation between the ammonia nitrogen concentration and the salinity in the Zhanjiang Bay during the monitoring period. The consumption of samples and reagents during the detection and analysis process is very small (μL), and the reaction time is short (60 s, salinity: 5‰), and these features make the analyzer ideally suited for long time series monitoring of ammonia nitrogen concentration in estuarine waters.

Key words: ammonia nitrogen, sequential injection analysis, incomplete color rendering, rapid measurement, spectrophotometry

HE Jiawei, YANG Zeming, ZHAO Jincheng, ZHANG Xianqing, LI Cai, LI Zhihao, LIN Yongquan, CHEN Rong. In situ rapid analytical method for ammonia nitrogen concentration in estuarine zone^*[J].Journal of Tropical Oceanography, 2024, 43(4): 144-152.

Figures/Tables 11

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References 23

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盐度/‰	显色反应时间/s	R²
5	30	0.9558
	60	0.9960
	90	0.9925
	120	0.9658
	150	0.9499
	180	0.9923
10	30	0.9855
	60	0.8522
	90	0.9932
	120	0.9594
	150	0.9431
	180	0.6930
15	30	0.9744
	60	0.9478
	90	0.9592
	120	0.9910
	150	0.9648
	180	0.7663
20	30	0.5760
	60	0.5031
	90	0.6916
	120	0.7983
	150	0.9939
	180	0.9391
25	30	0.9466
	60	0.9772
	90	0.8984
	120	0.9823
	150	0.9901
	180	0.9924

清洗次数	携带效应系数	第1次吸光度 (空白样)	第2次吸光度 (高浓度样品)	第3次吸光度 (空白样)
2	0.079	0.24	2.04	0.40
3	0.040	0.27	1.98	0.35
4	0.010	0.18	2.07	0.16
5	0.008	0.21	1.21	0.20
6	0.003	0.34	1.94	0.34

次数	Abs695	次数	Abs695	SD
1	0.23	7	0.20	0.02
2	0.18	8	0.17
3	0.21	9	0.19
4	0.18	10	0.18
5	0.15	11	0.16
6	0.17

次数	Abs695	次数	Abs695	RSD
1	0.76	7	0.91	7.6%
2	0.75	8	0.89
3	0.81	9	0.87
4	0.89	10	0.94
5	0.86	11	0.93
6	0.91

In situ rapid analytical method for ammonia nitrogen concentration in estuarine zone^*

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In situ rapid analytical method for ammonia nitrogen concentration in estuarine zone^*