深海微生物原位自动序列培养装置的研发与试验

doi:10.11978/2023166

Abstract

Abstract:

The global carbon and nitrogen cycles depend heavily on deep-sea microorganisms, and the majority of current, refined research on these organisms is based on high-pressure simulation culture and detection in laboratories. However, when deep-sea samples are brought in for culture and detection, the environmental parameters, such as temperature, pressure, etc, change at various degrees, which has an impact on the results of subsequent experiments and detection. For this reason, a device (ocean automatic series incubation system, OASIS) was developed for in situ automatic series incubation of deep-sea microorganisms. This research emphasizes the accuracy of deep-sea liquid transfer and focuses on the OASIS operating concept, system design, and experimental validation. The device can function normally under 30 MPa high pressure, and it can automatically finish the culture of microorganisms and the fixation of life information in accordance with the chronological order. These test results demonstrate that OASIS has passed the pressure, sea, pool, and other tests. They also accurately provide an understanding of the ecological benefits of deep-sea ecosystems. It offers strong equipment assistance for precisely identifying the ecological advantages of deep-sea environments.

Key words: in situ experiment, automation equipment, system design, serialization

DONG Zizhen, LIU Chunhu, ZHANG Yu. Development and testing of a deep-sea microorganism Ocean Automatic Series Incubation System[J].Journal of Tropical Oceanography, 2024, 43(5): 131-142.

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参数名词	技术指标	参数名词	技术指标
最大工作深度	6000m	单个固定管最大容积	150mL
最大工作时长	>72h	培养舱单次最大容积	3000mL
固定管总数量	18个	单台装置空气中总重	55kg
样品转移精度	±2mL	压力传感器检测精度	±0.1MPa

序号	密封填料材质	开启过程扭矩/(N∙m)	关闭过程扭矩/(N∙m)	拧紧力矩/(N∙m)	试验现象
1	A-UPE B-PTFE	7.0	5	9.0	保压30min出现泄露
2	A-UPE B-PTFE+玻璃纤维	6.5	5	8.0	保压4h无泄漏, 但阀针运行有异响
3	A-UPE B-PTFE+石墨	7.5	6	9.5	保压4h无泄漏, UPE填料明显磨损
4	U型圈	6.0	5	8.0	保压24h无泄漏, U型圈无明显磨损

潜次	经度/°E	纬度/°N	深度/m	试验时长/h	获取样品体积/mL	固定管取样误差/%
1	110°35′ 34.80″	18° 38′ 36.24″	103	4	2691	-2.0~0.7
2	111°20′ 27.96″	18° 05′ 48.48″	1874	8	2716	0~2
3	111° 43′ 15.60″	18° 13′ 01.20″	1614	8	2707	-1.3~1.3
4	111° 06′ 44.64″	19° 07′ 12.72″	109	4	2412	0~1.3

Development and testing of a deep-sea microorganism Ocean Automatic Series Incubation System

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