巴布亚硝水母(Mastigias papua)的精巢发育和精子超微结构观察

doi:10.11978/2024002

Abstract

Abstract:

In this study, Mastigias Papua was selected as the sample, its testis development and spermatozoa ultrastructure was observed in great detail. The development phases of testis and the ultrastructure of the spermatozoa were also studied. The results showed that the nest of M. papus with a bell diameter of (4.99±0.07)cm gradually became matured after 26d-40d under experimental conditions, and the spermatozoa bundle could be continuously discharged for 2-3 months. According to the structural characteristics of spermatozoa follicles and spermatogenesis, the development of the testis can be divided into three stages, namely stage I: the outer part of the testis is not visible to naked eye, the seminal vesicles are small and transparent, and are arranged loosely, and there is no cavity inside; Phase II: the testis is circular, milky white, with gradient development of the gonads. The seminal vesicles that are far from the gastric filament are larger and more tightly arranged, with an internal cavity; Stage III: The folds of the testis are clearly sac-shaped, and under light microscopy the seminal vesicles at different developmental stages can be seen. A large number of seminal vesicles are filled with mature spermatozoa, present in spermbundles. The spermatozoa of M. papus was (50.64±3.93)μm in length, including a sharp chilli shape head (7.61±0.63)μm, a slightly protruding middle section (1.00±0.12)μm. and a very slender tail (43.03±4.02)μm. There was no acrosome in the spermatozoa head, which was mainly composed of nucleus and thin plasma membrane, small vesicles scattered in the plasma membrane. The middle section is composed of two large mitochondria and is connected to the tail by anchoring apparatus. The internal axial filament of the spermatozoa tail was a typical 9+2 duplex microtubule structure.

Key words: Mastigias papua, testis development, spermatozoa ultrastructure

CLC Number:

Q958.8

ZHANG Ruixue, YANG Wenhao, CHEN Nan, WANG Shuhong. Development of testis and ultrastructure of spermatozoa in Mastigias papua[J].Journal of Tropical Oceanography, 2024, 43(6): 129-139.

Figures/Tables 11

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References 39

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精巢分期	外部形态	光学显微镜下	组织切片
Ⅰ期	肉眼不可见(图1a)	精小囊小而透明, 排列松散(图2)	由数个细胞核明显的精原细胞组成的球状精小囊, 内部无空腔(图5a、b)
Ⅱ期	环状乳白色, 由近胃丝处向外性腺透明度降低、颜色加深(图1b)	梯度发育, 由近胃丝处向外精小囊个体变大, 透明度降低(图3)	远离胃丝的精小囊更大, 排列更紧密, 内部有空腔, 可见明显的由精原细胞整齐排列而成的精小囊壁(图5c、d)
Ⅲ期	褶皱呈囊状, 浅米黄色(图1c)	处于不同发育阶段的精小囊, 正在进行精子发生的精小囊占绝大多数(图4)	精小囊内有不同发育阶段的配子细胞, 大量精小囊内充满以精子束存在的成熟精子(图5e、f)

物种	精子头部形态	头部长度/μm	尾部长度/μm	参考文献
夜光游水母	短而粗	<4.00	—	Franzén(1967)
海月水母	长尖辣椒状	4.15	27.78	Rouse等(2000)
狮鬃水母	细长	14.00	—	Arai(1997)
马赛克水母	短而粗	2	28.00	Rouse等(2000)
安朵仙水母	略微椭圆	4.00	35.00	Arai(1997)
煎蛋水母	圆柱形	6.00	—	Franzén(1967)
澳洲斑点水母	细长	6.00	42.00	Rouse等(2000)
巴布亚硝水母	尖辣椒状	7.61±0.63	43.03±4.02	本文章

Development of testis and ultrastructure of spermatozoa in Mastigias papua

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