一株海洋来源可降解聚氨酯的伊朗假单胞菌SCSIO 85019的分离鉴定及其降解特性

doi:10.11978/2025084

Abstract

Abstract:

Polyurethane (PU) plastics are widely utilized across various industries owing to their unique properties. However, their accumulation in marine environments has resulted in the generation of substantial amounts of microplastics, posing serious threats to marine ecosystems and human health. In this study, a PU-degrading strain, SCSIO 85019, was isolated and purified from marine sediments collected from Daya Bay, Huizhou. Based on colony morphological characteristics and 16S rRNA gene phylogenetic analysis, the strain was identified as Pseudomonas iranensis. Degradation capability assessments showed that this strain could degrade both aqueous polyester-polyurethane Impranil^TM DLN and solid polyester-polyurethane (poly1, 4-butylene adipate-based PU, PBA-PU) on minimal salt medium (MSM) plates, forming distinct hydrolysis zones with ratios of halo diameter to colony diameter (H/C) of 1.6 and 2.0, respectively. In liquid MSM culture, strain SCSIO 85019 achieved a degradation rate of 82.04% for 0.1% (V/V) Impranil^TM DLN after 4 days of incubation at 30°C and pH 7.0. LC-MS analysis of PBA-PU degradation products identified five intermediate metabolites, including adipic acid, 1, 4-butanediol, and 4, 4'-methylenedianiline derivatives, indicating that this strain could hydrolyze both the ester and urethane bonds of PBA-PU. Through extracellular enzyme activity assays and genomic bioinformatics analysis, we identified three putative esterases (QXI20836.1, QXI21523.1, and QXI24368.1) potentially involved in ester bond hydrolysis, along with one urease (QXI23417.1) and one protease (QXI25044.1) likely responsible for urethane bond cleavage. A potential pathway for PBA-PU degradation by strain SCSIO 85019 is proposed. This study not only reports the first evidence of polyurethane degradation by Pseudomonas iranensis but also characterizes the degradation properties of strain SCSIO 85019, thereby providing a valuable microbial resource for marine microplastic remediation.

Key words: polyurethane, marine microplastics, biodegradation, urease, esterase

CLC Number:

LIU Yuqing, WU Tingbiao, DENG Shijing, FAN Tingting, TIAN Xinpeng, LI Qinglian. Isolation, identification and degradation characteristics of a marine-derived polyurethane-degrading Pseudomonas iranensis strain SCSIO 85019[J].Journal of Tropical Oceanography, 2026, 45(3): 129-142.

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References 24

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时间/min	流速/(mL·min^-1)	流动相A/%	流动相B/%
0.04	0.2	85	15
1.2	0.2	5	95
4	0.2	5	95
4.04	0.2	85	15
6	0.2	85	15

酶	推测功能	序列号	预测EC编号
酯酶	SDR家族氧化还原酶	QXI20836.1	EC:3.1.1.1
	双烯内酯水解酶家族蛋白	QXI21523.1	EC:3.1.1.45
	双烯内酯水解酶家族蛋白	QXI24368.1	EC:3.1.1.45
脲酶	脲酶alpha亚基	QXI23417.1	EC:3.5.1.5
蛋白酶	丝氨酸蛋白酶	QXI25044.1	EC:3.4.25.2

Isolation, identification and degradation characteristics of a marine-derived polyurethane-degrading Pseudomonas iranensis strain SCSIO 85019

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