34株副溶血弧菌16S-23S rDNA间区多态性DGGE分析

doi:10.11978/j.issn.1009-5470.2010.03.055

Journal of Tropical Oceanography ›› 2010, Vol. 29 ›› Issue (3): 55-60.doi: 10.11978/j.issn.1009-5470.2010.03.055cstr: 32234.14.j.issn.1009-5470.2010.03.055

• Marine biology • Previous Articles Next Articles

Analysis of the polymorphism of 16S-23S rDNA intergenic spacer regions from 34 Vibrio parahaemolyticus strains by denaturing gradient gel electrophoresis

SU Ting, LUO Peng, HU Chao-qun, REN Chun-hua

South China Sea Institute of Oceanology, Key Laboratory of Marine Bio-resources Sustainable Utilization, cas, Key Laboratory of Applied Marine Biology of Guangdong, Guangzhou 510301

Received:2008-03-25 Revised:2008-12-02 Online:2010-07-01 Published:2010-05-24
About author:苏婷(1985—), 女, 广西桂林市人, 硕士研究生, 从事海洋微生物学研究.
Supported by:
国家“973”计划项目(2006CB101803); 国家自然科学基金(30700016)

Abstract

Abstract:

Using PCR-denaturing gradient gel electrophoresis (PCR-DGGE), the polymorphism of 16S-23S rDNA intergenic spacer regions (ISR) from 34 Vibrio parahaemolyticus strains, including type strains and those isolated from environment and aquatic animals, were analyzed. The phylogenic relationships of these strains were also analyzed. The results showed that the ISRs could be separated into 4−10 different bands through PCR-DGGE. Thirty-four V. parahaemolyticus strains shared 15 polymorphic sites. Using MVSP, 34 strains were clustered into four groups, H, I, J, and K. Strains A18 and A25 had farthest genetic distance, with a value of 0.4. The present study suggested that ISR PCR-DGGE provided a new method for genotyping of V. parahaemolyticus.

Key words: Vibrio parahaemolyticus, 16S-23S rDNA intergenic spacer Regions, PCR-DGGE

SU Ting,LUO Peng,HU Chao-qun,REN Chun-hua. Analysis of the polymorphism of 16S-23S rDNA intergenic spacer regions from 34 Vibrio parahaemolyticus strains by denaturing gradient gel electrophoresis[J].Journal of Tropical Oceanography, 2010, 29(3): 55-60.

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Analysis of the polymorphism of 16S-23S rDNA intergenic spacer regions from 34 Vibrio parahaemolyticus strains by denaturing gradient gel electrophoresis

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