硒对紫球藻抗氧化酶活性及丙二醛含量的影响

doi:10.11978/j.issn.1009-5470.2011.03.094

Abstract

Abstract:

The effect of selenium on Porphyridium sp. UTEX 637 major antioxidant enzyme system, including glutathione
peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) activities, and malondialdehyde (MDA) content, were
investigated. Porphyridium sp. was cultured in artificial seawater ASW medium containing 0.5mg·L−1
selenium. GSH-Px,
SOD, CAT activities, and MDA content were determined by kits, nitroblue tetrazolium (NBT) photoreduction, UV spectro-
photometry, and thiobarbituric acid (TBA) analysis, respectively. The results show that selenium improved the antioxidation
activity of Porphyridium sp. by increasing the activity of GSH-Px (p<0.05). The GSH-Px activity of Se-treated group and
control group reached the highest on the 15th day, and the GSH-Px activity of Se-treated group was heightened to 4.31U,
which was 1.31 fold higher than that of the control group. SOD and CAT activities reached the maximum on the 18th day and
15th day, respectively, but the differences between the experimental group and the control were not significant (p>0.05). The
activity of SOD and CAT was not significant at the 0.5mg·L−1
concentration of selenium. The content of MDA in Se-treated
group was 15.21% lower than that in the control group.

Key words: Porphyridium sp., selenium, antioxidant enzyme, malonaldehyde

WANG Jiang-hong,LIU Yan,SANG Min,LI Ai-fen,ZHANG Cheng-wu. Effect of selenium on antioxidant enzyme activity and malondialdehyde content of Porphyridium sp.[J].Journal of Tropical Oceanography, 2011, 30(3): 94-98.

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Effect of selenium on antioxidant enzyme activity and malondialdehyde content of Porphyridium sp.

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