大珠母贝(Pinctada maxima)α-淀粉酶基因cDNA及内含子克隆分析

doi:10.11978/j.issn.1009-5470.2013.03.008

Journal of Tropical Oceanography ›› 2013, Vol. 32 ›› Issue (3): 52-58.doi: 10.11978/j.issn.1009-5470.2013.03.008cstr: 32234.14.j.issn.1009-5470.2013.03.008

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Cloning and characterization of alpha amylase cDNA and its introns in the pearl oyster Pinctada maxima

PAN Li-ling^{1, 2}, HUANG Gui-ju¹, CHENG Shu-ying^{1, 2}, WANG Xiao-ning^{1, 2}, YU Da-hui¹

1. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, P. R. China, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; 2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

Received:2012-01-13 Revised:2012-12-10 Online:2013-08-27 Published:2013-08-28
Contact: 喻达辉，博士，博导，研究员。E-mail：pearlydh@163.com

Abstract

Abstract:

Alpha amylase is widely distributed in animals, plants and bacteria, and is one of the important digestive enzymes and has a significant effect on the growth of mollusk. It is the first time the alpha amylase gene in silver lipped pearl oyster Pinctada maximais cloned. The full length of the cDNA (named as pmAMY) is consisted of 1732 bp, including a 5 UTR of 25 bp, an open reading frame (ORF) of 1554 bp encoding 518 amino acids and a 3 UTR of 153bp. The estimated isoelectric point and molecular mass of deduced amino acid residues are 7.63 and 57.7 kD, respectively. Amino acid sequence analysis indicates that pmAMY contains a signal peptide with 16 aimino acid residues (MLLIVCSIAFFHSVYG), 8 cysteine residues

Key words: Pinctada maxima, alpha amylase, exon, intron, sequence characteristics

CLC Number:

P735.54

PAN Li-ling, HUANG Gui-ju, CHENG Shu-ying, WANG Xiao-ning, YU Da-hui. Cloning and characterization of alpha amylase cDNA and its introns in the pearl oyster Pinctada maxima[J].Journal of Tropical Oceanography, 2013, 32(3): 52-58.

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Cloning and characterization of alpha amylase cDNA and its introns in the pearl oyster Pinctada maxima

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