黄斑篮子鱼去毒相关基因的克隆与肝脏组成型表达分析

doi:10.11978/j.issn.1009-5470.2009.06.079

Journal of Tropical Oceanography ›› 2009, Vol. 28 ›› Issue (6): 79-87.doi: 10.11978/j.issn.1009-5470.2009.06.079cstr: 32234.14.j.issn.1009-5470.2009.06.079

• Marine biology • Previous Articles Next Articles

Molecular cloning of cDNA core sequences of detoxification-related genes and liver constitutive expression in rabbitfish Siganus oramin

WANG Lin, LIANG Xu-fang, LIN Qun, Li Guang-zhao, Hu Yong-le

College of Life Science and Technology, Jinan University, Guangzhou 510632, China

Received:2007-11-28 Revised:2008-04-09 Online:2009-12-30 Published:2009-12-12
Contact: 梁旭方 E-mail:tliangxf@jnu.edu.cn
About author:王琳(1980— )，女，山西省人，博士，主要从事鱼类去毒相关基因研究工作。
Supported by:
国家科技部“863”项目(2007AA09Z437), 国家自然科学基金项目(30670367), 广东省科技计划项目(2007B020701002；2005B20301005), 广东省自然科学基金项目(031886)和教育部留学回国人员科研启动基金项目

Abstract

Abstract:

Partial cDNA sequences of hepatic cytochrome P-450 1A (CYP1A), glutathione S-transferase alpha (GSTA), glutathione S-transferase rho (GSTR), heat shock protein 70 (HSP70), Na+/K+-ATPase alpha 1(ATP1A1) and beta-actin (ACT) genes from rabbitfish (Siganus oramin) were obtained by PCR using degenerate primers. These sequences were 879 bp, 582 bp, 588 bp, 660 bp, 749 bp and 554 bp in length, respectively. Homology of the CYP1A, GSTA and GSTR amino acid sequence are high with bastard halibut (Paralichthys olivaceus), European plaice (Pleuronectes platessa), red sea bream (Pagrus major) and zebrafish CYP1A, GSTA and GSTR, and CYP1A and GSTA are low with human, rat, mouse, chicken and African clawed frog. The identities of HSP70, ATP1A and beta-actin amino acid sequences are highly conserved between fish, bird, amphibian and mammals. Using semi-quantitative PCR within the exponential phase, with beta-actin as an external control, the constitutive transcriptional expression of genes of phase I (CYP1A), phase II (GSTA and GSTR) xenobiotic metabolizing enzymes (XMEs), HSP70 and ATP1A1 were also investigated. The levels of CYP1A, GSTA and GSTR mRNA expression were higher, and the levels of HSP70 and ATP1A1 mRNA expression were lower. The constitutive expression pattern of these genes, show an accordant relationship with their functions, which provide data for our understanding to the detoxification mechanism of marine algae toxin in marine fish.

Key words: Cytochrome P-450 1A, glutathione S-transferase, Na+/K+-ATPase, heat shock protein 70, gene cloning, constitutive expression, rabbitfish (Siganus oramin)

WANG Lin,LIANG Xu-fang,LIN Qun,Li Guang-zhao,Hu Yong-le. Molecular cloning of cDNA core sequences of detoxification-related genes and liver constitutive expression in rabbitfish Siganus oramin[J].Journal of Tropical Oceanography, 2009, 28(6): 79-87.

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Molecular cloning of cDNA core sequences of detoxification-related genes and liver constitutive expression in rabbitfish Siganus oramin

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