Bacillus sp. SCSIO 15029中一种耐高温Mn-超氧化物歧化酶的克隆、表达及鉴定

doi:10.11978/2015125

Abstract

Abstract: A putative superoxide dismutase gene (BaSOD) was cloned from Bacillus sp. SCSIO 15029, which was isolated from the sediment of the South China Sea. The BaSOD gene was expressed in Escherichia coli BL21(DE3) by using pET-28a(+) expression system. The recombinant enzyme was soluble in E. coli, and its biochemical properties were studied. BaSOD exhibits maximum activity at pH 7.5 and 40℃ with a specific activity of BaSOD at 5511.46 U·mg^-1. BaSOD is a manganese-dependent superoxide dismutase. The enzyme activity was basically not affected after incubation at 60℃ for 30 min. The residual activity was 71% after incubation at 70℃ for 30 min. BaSOD shows good thermo-stability and possesses very good potential for industrial utilization.

Key words: Bacillus, deep-sea thermophilic enzyme, superoxide dismutase

CLC Number:

Q939

DENG Dun, ZHANG Yun, SUN Aijun, HU Yunfeng. Cloning, expression and characterization of a thermo-tolerant manganese superoxide dismutase from Bacillus sp. SCSIO 15029[J].Journal of Tropical Oceanography, 2016, 35(4): 63-70.

References

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Cloning, expression and characterization of a thermo-tolerant manganese superoxide dismutase from Bacillus sp. SCSIO 15029

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