细胞破碎方法对Bacillus sp. DL-2胞内蛋白酶拆分乙酸苏合香酯的影响

doi:10.11978/2020041

Abstract

Abstract:

(R)-1-phenylethanol is a key chiral building block in the synthesis of chiral drugs, and plays important roles in the synthesis of many chiral compounds and in the industrial production of medicines and spices. The strain Bacillus sp. DL-2 screened from the deep-sea sediments of the western Pacific Ocean was broke, and the obtained intracellular proteases can asymmetrically hydrolyze (±)-1-phenylethyl acetate to obtain (R)-1-phenylethanol. Three different ways, namely, surfactant, lysozyme and ultrasonic, were utilized to crush Bacillus sp. DL-2. After the optimizations of the surfactant and lysozyme crushing methods, the optimal working conditions were found to be 0.5% TritonX-100, 35 ℃ and pH 7.0 for 2 h. After process optimization, (R)-1-phenylethanol was obtained with an enantiomeric excess of 96% and a conversion of 23%, which provide reference for using intracellular proteases to produce chiral chemicals.

Key words: surfactant, lysozyme, ultrasonication, kinetic resolution, (R)-1-phenylethanol

CLC Number:

P735.4

WANG Xiaomin, DONG Lu, ZHANG Jifu, ZHANG Yun, HU Yunfeng. The effects of different cell breaking methods on the asymmetrical hydrolysis of phenylethyl acetate using intracellular proteases of Bacillus sp. DL-2[J].Journal of Tropical Oceanography, 2021, 40(2): 39-48.

Figures/Tables 5

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The effects of different cell breaking methods on the asymmetrical hydrolysis of phenylethyl acetate using intracellular proteases of Bacillus sp. DL-2

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