不同绿藻对模拟酸雨胁迫的生理生化响应特征

doi:10.11978/2022243

Abstract

Abstract:

The maximal photochemical quantum yield of PSⅡ (F_v/F_m), soluble sugar contents and antioxidant enzyme activity were determined by using dominant species of chlorophyta in the intertidal zone of the East China Sea. The physiological and biochemical responses of dominant species of chlorophyta under simulated acid rain (pH 6.3, 5.6, 4.5, 4.0, 3.5, respectively) were studied. The results showed that: 1) the simulated acid rain inhibited F_v/F_m of Ulva prolifera and Ulva intestinalis, the remaining green seaweeds of Ulva, Cladophora stimpsonii and Codium fragile, and the inhibition amplitude increased with the increase of acidity and treatment time; 2) simulated acid rain at pH 4.5 reduced the contents of chlorophyll a, carotenoids and soluble sugar, and the decrease amplitude was green seaweeds of Ulva > C. fragile > C. stimpsonii. but the contents of soluble protein increased, which became more significant as time went on, and the increase amplitude of C. stimpsonii was higher than that of other green seaweeds; 3) simulated acid rain at pH 4.5 inhibited the activities of superoxide dismutase (SOD) and catalase (CAT), but increased the activities of peroxidase (POD) of seaweeds, and the treatment time was positively correlated with the activities of POD, but negatively correlated with the activities of SOD and CAT. In summary, pH 4.5 can be used as physiological and biochemical test conditions for dominant species of chlorophyta to simulated acid rain stress, C. stimpsonii exhibited higher tolerance to simulated acid rain, compared with U. prolifera and U. intestinalis, the remaining green seaweeds of Ulva has higher tolerance to simulated acid rain.

Key words: chlorophyta, simulated acid rain, dominant species, physiological and biochemical response, East China Sea

DU Xiang, LUO Qijun, CHEN Haimin. Physiological and biochemical responses of different species of chlorophyta to simulated acid rain stress[J].Journal of Tropical Oceanography, 2023, 42(5): 115-123.

Figures/Tables 7

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绿藻种类	0min	30min	60min	120min	240min	360min
浒苔	0.735±0.005 ^a	0.728±0.010^a	0.678±0.018^ab	0.627±0.060^bc	0.545±0.001^c	0.446±0.010^d
肠浒苔	0.479±0.005^a	0.416±0.014^b	0.401±0.017^bc	0.370±0.007^bc	0.356±0.022^cd	0.312±0.021^d
石莼	0.735±0.053^a	0.733±0.021^a	0.714±0.007^ab	0.642±0.078^abc	0.549±0.029^bc	0.525±0.066^c
长石莼	0.678±0.024^a	0.674±0.039^a	0.660±0.020^ab	0.634±0.021^ab	0.578±0.023^b	0.479±0.034^c
孔石莼	0.698±0.011^a	0.702±0.032^a	0.687±0.036^a	0.664±0.012^a	0.578±0.003^b	0.514±0.011^b
蛎菜	0.512±0.009^a	0.477±0.010^ab	0.444±0.034^ab	0.420±0.012^ab	0.410±0.008^ab	0.366±0.093^b
史氏刚毛藻	0.671±0.033^a	0.672±0.012^a	0.636±0.051^a	0.616±0.009^a	0.574±0.003^ab	0.505±0.039^b
刺松藻	0.675±0.009^a	0.671±0.014^a	0.660±0.018^a	0.646±0.006^a	0.586±0.016^b	0.499±0.016^c

绿藻种类	0min	30min	60min	120min	240min	360min
浒苔	0.444±0.017^a	0.442±0.028^ab	0.402±0.008^ab	0.377±0.031^b	0.303±0.021^c	0.281±0.002^c
肠浒苔	0.347±0.006^a	0.313±0.008^ab	0.305±0.012^b	0.284±0.008^bc	0.265±0.004^cd	0.228±0.021^d
石莼	0.470±0.033^a	0.475±0.002^a	0.450±0.019^a	0.421±0.051^ab	0.385±0.042^ab	0.332±0.033^b
长石莼	0.217±0.013^a	0.212±0.003^ab	0.188±0.012^bc	0.171±0.006^cd	0.155±0.003^d	0.147±0.009^d
孔石莼	0.567±0.031^a	0.541±0.001^ab	0.551±0.001^ab	0.508±0.003^b	0.448±0.001^c	0.406±0.007^c
蛎菜	0.284±0.017^a	0.274±0.011^a	0.270±0.001^a	0.249±0.002^a	0.233±0.014^a	0.212±0.053^a
史氏刚毛藻	0.396±0.041^a	0.392±0.009^a	0.388±0.008^ab	0.372±0.017^ab	0.365±0.022^ab	0.313±0.026^b
刺松藻	0.406±0.002^a	0.400±0.005^a	0.388±0.033^ab	0.361±0.007^ab	0.341±0.009^bc	0.302±0.006^c

Physiological and biochemical responses of different species of chlorophyta to simulated acid rain stress

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