Journal of Tropical Oceanography ›› 2020, Vol. 39 ›› Issue (3): 76-85.doi: 10.11978/2020003CSTR: 32234.14.2020003
• Marine Biology • Previous Articles Next Articles
Effect of manganese on the growth and chlorophyll fluorescence characteristics of benthic dinoflagellate Coolia tropicalis
Shasha LIU1,2, Heng CHEN1,2, Kaixuan HUANG1,2(
), Songhui LÜ1,2, Liang ZHANG3, Xuedong XIE3
- 1. Research Center for Harmful Algae and Marine Biology, Jinan University, Guangzhou 510362, China
2. Key Laboratory of Aquatic Eutrophication and Control of Harmful Algae Blooms of Guangdong Higher Education Institutes, Guangzhou 510632, China
3. Guangdong Environmental Monitoring Center, Guangzhou 510362, China
-
Received:2020-01-08Revised:2020-01-29Online:2020-05-10Published:2020-05-19 -
Contact:Kaixuan HUANG E-mail:babyblue02@163.com -
Supported by:Foundation item: National Natural Science Fund ation of China(41706126);National Natural Science Fund ation of China(41876173);Science & Technology Basic Resources Investigation Program of China(2018FY100200);Science & Technology Basic Resources Investigation Program of China(2018FY100100)
Cite this article
Shasha LIU, Heng CHEN, Kaixuan HUANG, Songhui LÜ, Liang ZHANG, Xuedong XIE. Effect of manganese on the growth and chlorophyll fluorescence characteristics of benthic dinoflagellate Coolia tropicalis[J].Journal of Tropical Oceanography, 2020, 39(3): 76-85.
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Tab. 1
The coefficients of JIP-test"
| 参数及其计算 | 定义 |
|---|---|
| F0 | 暗适应后的最小荧光强度 |
| Fm=FP | 暗适应后的最大荧光值 |
| VJ = (FJ - F0) / (Fm - F0) | 在J点的相对可变荧光 |
| VI = (FI - F0) / (Fm - F0) | 在I点的相对可变荧光 |
| Fv / F0 = (Fm - F0) -1 | PSⅡ的潜在活性 |
| Fv / Fm | PSⅡ最大光化学效率 |
| M0 = 4 (F300μs - F0) / (Fm - F0) | OJIP荧光诱导曲线的初始斜率 |
| Area | 荧光诱导曲线与F=Fm之间的面积 |
| Sm = Area / (Fm - F0) | 标准化后的在OJIP荧光诱导曲线和F=Fm之间的面积 |
| ABS / RC = M0 (1/VJ) · (1/φP0) | 单位反应中心吸收的光能 |
| TR0 / RC = M0 (1/VJ) | 单位反应中心捕获的用于还原QA的能量(在t=0时) |
| ET0 / RC = M0 (1/VJ)ψ0 | 单位反应中心捕获的用于电子传递的能量(在t=0时) |
| DI0 / RC = (ABS / RC) - (TR0 / RC) | 单位反应中心的热耗散(在t=0时) |
| φP0 = TR0 / ABS =(1- F0/Fm) | 最大光化学效率(在 t=0 时) |
| φ0 = ET0 / TR0 =(1- VJ) | 捕获的激子将电子传递到电子传递链中超过 QA 的其它电子受体的概率(在t=0时) |
| φE0 = ET0 / ABS =(1- F0 / Fm ) φ0 | 用于电子传递的量子产额(在t=0时) |
| φD0 = 1- φP0 = (F0 / Fm ) | 用于热耗散的量子比率(在t=0时) |
| PIABS = RC/ABS×[φP0 /(1- φP0 )] [φ0/ (1- φ0 )] | 以吸收光能为基础的性能指数 |
Tab. 1
Fig. 1
Cell density (a) and the effective photochemical efficiency (b) of C. tropicalis under different Mn treatment"
Fig. 1
Fig. 2
Growth rate (μ) of C. tropicalis under different Mn treatment"
Fig. 2
Fig. 3
RLCs (a) and NPQ (b) of C. tropicalis under different Mn treatment"
Fig. 3
Fig. 4
Maximal electron transport rate (rETRmax)(a), light saturate coefficient (Ek) (b) and maximal light use coefficient (α) (c) calculated by RLCs of C. tropicalis under different Mn treatment"
Fig. 4
Tab. 2
Initial slopes and saturated manganese concentrations of μ, rETRmax, Ek, and α"
| 参数 | 初始斜率 | 饱和锰浓度/(μmol·L-1) |
|---|---|---|
| μ/(d-1) | 0.24 ± 0.03 | 48.66 ± 5.49 |
| rETRmax/(μmol e-·m-2·s-1) | 0.25 ± 0.02 | 17.29 ± 1.12 |
| Ek/(μmol photons·m-2·s-1) | 0.09 ± 0.04 | 49.74 ± 19.67 |
| α | 0.31 ± 0.03 | 15.72 ± 1.47 |
Tab. 2
Fig. 5
Changes of chlorophyll fluorescence induction parameters of C. tropicalis under different Mn treatment on days 6 and 15. a) minimal fluorescence intensity; b) maximal fluorescence intensity; c) relative variable fluorescence intensity at the J-step; d) relative variable fluorescence intensity at the I-step; e) potential activity of PSⅡ; f) maximal photochemical efficiency; g) normalised total complementary area above the O-J-I-P transie; h) approximated initial slope of the fluorescence transient"
Fig. 5
Fig. 6
Changes of ABS/RC, DI0/RC, ET0/RC, TR0/RC, φ0, φD0, φE0, and PIABS under different Mn treatment on days 6 and 15. a) absorption flux per reaction center; b) dissipated energy flux per reaction center; c) electron transport flux per reaction center; d) trapped energy flux per reaction center; e) probability that a trapped exciton moves an electron into the electron transport chain beyond QA-; f) quantum yield for energy dissipation; g) quantum yield for electron transport; h) performance index on absorption basis"
Fig. 6
Fig. 7
Changes in the photochemical efficiency of C. tropicalis under different Mn treatment after 1000μmol photons·m-2·s-1 (a) and 15μmol photons·m-2·s-1 (b)"
Fig. 7
Fig. 8
The repair rate to damage rate (r/k) of C. tropicalis under different Mn treatment after 1000 and 15μmol photons·m-2·s-1, respectively"
Fig. 8
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