牡蛎酶解产物对产后缺乳症泌乳功能改善作用探究

doi:10.11978/2022075

Abstract

Abstract:

In this paper, oyster hydrolysis products were prepared from Crassostrea hongkongensis as raw materials, and the basic nutrition and amino acid composition of oyster hydrolysis products were analyzed, and the improvement effect of oyster hydrolysis products on the lactation function of rats with postpartum lactation caused by Bromocriptine mesylate was explored. The results showed that protein content of oyster hydrolysis products was higher, which is followed by sugar, fat content was lower, and peptide content was 33.4%. The essential amino acids in oyster hydrolysis products accounted for 39.8% of the total amino acids and contained a high proportion of branched amino acids. The results of animal experiment showed that compared with model group, the lactation volume of rats in oyster hydrolysis products of high-dose group was significantly increased (p < 0.05), which was better than that in the positive control group. The index of mammary tissue and organ in each oyster hydrolysis products dose group was significantly increased (p < 0.01), which was close to the positive control group, but did not return to normal level. HE staining showed that there were more acinus in mammary gland tissues in all oyster hydrolysis products dose groups, which were round and full with more secretions, and the acinus had obvious enlargement and hyperplasia. Compared with the model group, the acinus had obvious recovery. Compared with model group, the contents of prolactin (PRL) and prolactin receptor (PRLR) in serum and mammary tissue of oyster hydrolysis products groups were largely increased (p < 0.05), indicating that oyster hydrolysis products could promote the secretion of prolactin in lactation-deficient rats, while the content of serum dopamine (DA) was greatly decreased (p < 0.01). These results showed that oyster hydrolysis products can reduce the high level of dopamine induced by bromocystine. In addition, oyster hydrolysis products strongly increased the contents of lactose (LC) and triglyceride (TG) in breast tissue (p < 0.05), and improved the nutritional composition of milk. The average hemoglobin (MCHC) and red blood cell (RBC) contents in all oyster hydrolysis products dose groups were also greatly increased (p < 0.05), which had a certain improvement effect on postpartum deficiency of qi and blood. Conclusion: Oyster hydrolysis products can improve the symptoms of lactation deficiency and increase milk yield after delivery, which has the potential to promote lactation.

Key words: oyster hydrolysates, postpartum lactation deficiency, bromocriptine mesylate, amount of lactation

WEI Xue, QIN Xiaoming, CHEN Suhua, ZHANG Kaijia, LIN Haisheng, ZHENG Huina, GAO Jialong. Study on the effect of Oyster hydrolysates on improving Lactation function in Postpartum hypogalactism[J].Journal of Tropical Oceanography, 2023, 42(2): 141-152.

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组别	母鼠数量/只	每窝仔鼠数量/只	处理方式
空白组(B组)	6	12	用1mL·100g^-1蒸馏水灌胃, 每天一次
模型组(M组)	6	12	同时用甲磺酸溴隐亭溶液(浓度0.5mg·mL^-1、体积1mL·100g^-1)和1mL·100g^-1蒸馏水灌胃, 每天一次
阳性药物组(P组)	6	12	同时用甲磺酸溴隐亭溶液(浓度0.5mg·mL^-1、体积1mL·100g^-1)和补血生乳颗粒(浓度267mg·mL^-1、体积1mL·100g^-1)灌胃, 每天一次
牡蛎酶解产物低剂量组(OH-L组)	6	12	同时用甲磺酸溴隐亭溶液(浓度0.5mg·mL^-1、体积1mL·100g^-1)和牡蛎酶解粉(浓度31.25mg·mL^-1、体积1mL·100g^-1)灌胃, 每天一次
牡蛎酶解产物中剂量组(OH-M 组)	6	12	同时用甲磺酸溴隐亭溶液(浓度0.5mg·mL^-1、体积1L·100g^-1)和牡蛎酶解粉(浓度62.5mg·mL^-1、体积1 mL·100g^-1)灌胃, 每天一次
牡蛎酶解产物高剂量组(OH-H 组)	6	12	同时用甲磺酸溴隐亭溶液(浓度0.5mg·mL^-1、体积1mL·100g^-1)和牡蛎酶解粉(浓度125mg·mL^-1、体积1mL·100g^-1)灌胃, 每天一次

种类	水解氨基酸含量/(g·100g^-1)	游离氨基酸含量/(g·100g^-1)
天门冬氨酸(ASP)	3.81	0.74
苏氨酸(THR)*	1.67	1.37
丝氨酸(SER)	1.80	1.00
谷氨酸(GLU)	5.94	1.97
脯氨酸(PRO)	1.46	0.59
甘氨酸(GLY)	2.45	0.65
丙氨酸(ALA)	2.20	1.56
缬氨酸(VAL)*	2.16	1.30
蛋氨酸(MET)*	1.34	0.97
异亮氨酸(ILE)*	1.79	1.43
亮氨酸(LEU)*	2.79	2.39
酪氨酸 TYR	1.04	2.09
苯丙氨酸(PHE)*	1.57	2.02
赖氨酸(LYS)*	2.84	0.92
组氨酸(HIS)	0.48	0.27
精氨酸(ARG)	2.31	1.52
氨基酸总和(TAA)	35.60	20.80
必需氨基酸(EAA)	14.16	10.40
非必需氨基酸(NEAA)	21.44	10.40
EAA与TAA比值/%	39.80	50.00
EAA与NEAA比值/%	66.00	100.00

指标	B组	M组	P组	OH-L组	OH-M组	OH-H组
心脏指数	0.45±0.11	0.43±0.03	0.43±0.05	0.40±0.03	0.44±0.05	0.43±0.06
胸腺指数	0.09±0.02	0.11±0.02	0.12±0.01	0.11±0.01	0.11±0.02	0.12±0.02
脾脏指数	0.22±0.01	0.24±0.02	0.24±0.02	0.23±0.01	0.25±0.02	0.25±0.04
肝脏指数	3.94±0.42	3.41±0.37	3.20±0.22	3.20±0.24	3.28±0.21	3.41±0.21
肾脏指数	0.70±0.07	0.74±0.07	0.74±0.04	0.75±0.08	0.72±0.06	0.71±0.05

时间	B组	M组	P组	OH-L组	OH-M组	OH-H组
第1天	2.28±0.73	1.49±0.40^##	3.35±0.50^**	1.75±1.94	2.51±0.48^**	2.93±0.50^**
第2天	2.29±1.04	2.57±1.04	2.07±0.86	0.81±1.82	1.21±0.41	3.19±0.32
第3天	4.11±0.79	1.98±1.26^#	1.60±0.77	2.33±0.77	0.89±0.83	2.02±0.57
第4天	4.46±0.56	0.90±1.13^##	2.17±0.77	0.71±1.42	1.99±0.88	1.74±0.12
第5天	6.38±1.15	0.44±0.48^##	1.05±0.98	1.77±1.24	0.67±0.41	2.47±1.00^**
第6天	6.20±1.1	0.03±0.48^##	1.82±0.31^**	1.05±1.39	0.13±0.66	2.79±0.89^**
第7天	4.88±1.62	0.39±0.51^##	0.05±0.79	0.57±1.29	1.00±0.79	3.10±0.85^**
第8天	4.74±1.45	0.05±0.10^##	1.64±2.10^**	0.93±1.48	0.18±0.23	1.11±0.70

组别	第3天	第4天	第5天	第6天	第7天	第8天
B组	100±0.00	100±0.00	100±0.00	100±0.00	100±0.00	100±0.00
M组	100±0.00	100±0.00	94.44±8.61	88.88±6.21^##	84.72±3.40^##	77.78±4.30^##
P组	100±0.00	100±0.00	100±0.00	98.61±3.40^*	95.84±4.56^**	87.50±4.57^**
OH-L组	100±0.00	97.22±4.30	98.61±3.40	98.61±3.40^*	87.50±4.57^##△△	81.94±3.40^△
OH-M组	100±0.00	100±0.00	98.61±3.40	95.84±4.56	94.45±4.30^**	84.72±3.40^**
OH-H组	100±0.00	100±0.00	100±0.00	100±0.00^**	98.61±3.40^**	93.06±3.40^**△

Study on the effect of Oyster hydrolysates on improving Lactation function in Postpartum hypogalactism

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