香港牡蛎在综合养殖池塘育肥期间不同组织的碳、氮稳定同位素周转

doi:10.11978/2022175

Abstract

Abstract:

In order to provide basic data for the study of food webs in the polyculture pond ecosystem, the Hongkong oyster samples were collected during fattening stage in a fish-shrimp polyculture pond, and the oyster body mass, soft tissue mass, meat yield, and changes in δ¹³C and δ¹⁵N values of adductor muscle, mantle and ctenidia during the fattening stage were monitored; the metabolic rate constant m and stable carbon and nitrogen isotope turnover time of three soft tissues were calculated. The results showed that after 44 days of fattening, although the body mass of oysters did not increase significantly (P>0.05), the soft body tissue mass and meat yield increased significantly(P<0.05). The growth of mantle, adductor muscle and ctenidia were basically synchronized with the growth of whole soft tissue. The δ¹³C and δ¹⁵N values of oyster tissues decreased significantly (P<0.05) after 16 days of fattening (P<0.05). Comparing the δ¹³C and δ¹⁵N values of the three tissues collected on the same day, the values of adductor muscle were significantly larger than those of mantle and ctenidia (P<0.05), while there was no significant difference between values of mantle and ctenidia (P>0.05). The metabolic turnover constant m of adductor muscle and mantle was zero, and their stable carbon and nitrogen isotope turnover were completely determined by growth; therefore the turnover time of stable carbon and nitrogen isotope turnover in adductor muscle and mantle were the same, t₅₀ and t₉₅ were 40.77d and 176.22d, respectively. The stable carbon and nitrogen isotope metabolic turnover constant m of ctenidia were 1.09%·d^-1 and 1.76%·d^-1, respectively, indicating that the stable carbon and nitrogen isotope turnover of ctenidia were also affected by metabolism. The t₅₀ and t₉₅ of ctenidia stable nitrogen isotope turnover were 20.02d and 86.53d, respectively, while the t₅₀ and t₉₅ of stable carbon isotope turnover were 24.85d and 107.42d, respectively. During the fattening stage of this study, the δ¹³C and δ¹⁵N values of the adductor muscle, mantle and ctenidia of Hongkong oyster had distinct characteristics, and showed rapid and regular changes with the extension of the fattening time, which indicated the Hongkong oyster could reflect the basic characteristics of stable carbon and nitrogen isotopes in polyculture pond, and is suitable as a medium and long time scale baseline organism for food web research in polyculture pond ecosystem.

Key words: Crassostrea hongkongensis, fattening, stable carbon and nitrogen isotopes, turnover

ZHOU Hui, TANG Baogui, WU Xumin, PENG Zifeng, ZHONG Peigui, YU Ge, KONG Fansen. Stable carbon and nitrogen isotope turnover of different tissues of the Hongkong oyster at the fattening stage in a polyculture pond[J].Journal of Tropical Oceanography, 2023, 42(4): 125-132.

Figures/Tables 6

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References 28

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项目		初始样品	育肥8d	育肥16d	育肥44d
外套膜	δ¹⁵N/‰	9.84±0.40^c	9.70±0.82^c	8.59±0.53^b	7.38±0.22^a
外套膜	δ¹³C/‰	-21.81±1.21^c	-22.09±0.94^c	-22.38±0.81^b	-24.45±0.39^a
闭壳肌	δ¹⁵N/‰	10.93±0.23^c	10.70±0.73^c	9.71±0.76^b	8.64±0.50^a
闭壳肌	δ¹³C/‰	-20.07±0.17^c	-20.45±0.53^bc	-20.97±0.79^b	-22.16±0.63^a
鳃	δ¹⁵N/‰	9.97±0.17^c	9.50±0.69^c	8.24±0.34^b	7.34±0.51^a
鳃	δ¹³C/‰	-21.28±0.48^c	-22.08±0.57^bc	-22.64±0.33^b	-24.00±0.65^a

项目		初始样品	育肥8d	育肥16d	育肥44d
δ¹⁵N/‰	外套膜	9.84±0.40^a	9.70±0.82^a	8.59±0.53^a	7.38±0.22^a
	闭壳肌	10.93±0.23^b	10.70±0.73^b	9.71±0.76^b	8.64±0.50^b
	鳃	9.97±0.17^a	9.50±0.69^a	8.24±0.34^a	7.34±0.51^a
δ¹³C/‰	外套膜	-21.81±1.21^a	-22.09±0.94^a	-22.38±0.81^a	-24.45±0.39^a
	闭壳肌	-20.07±0.17^b	-20.45±0.53^b	-20.97±0.79^b	-22.16±0.63^b
	鳃	-21.28±0.48^a	-22.08±0.57^a	-22.64±0.33^a	-24.00±0.65^a

Stable carbon and nitrogen isotope turnover of different tissues of the Hongkong oyster at the fattening stage in a polyculture pond

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