热带海草泰来草沉积物真菌的群落结构、功能与分子生态网络研究

doi:10.11978/2022226

Abstract

Abstract:

Fungi are essential components of seagrass ecosystems, and they play important roles in maintaining seagrass health and nutrient cycling in the ecosystem. To elucidate the fungal community structure and their functions in seagrass sediment, we used Illumina MiSeq high-throughput sequencing technique to investigate the fungi in sediments of tropical seagrass Thalassia hemprichii in Hainan Island and Xisha Islands, respectively. FUNGuild database was introduced to predict fungi trophic types and annotate fungi guilds. Results showed that phylum Ascomycota (relative abundance 24.30% ~ 76.20%) and Basidiomycota (relative abundance 4.98% ~ 52.24%) were the dominant phyla in the two study areas, but the relative abundance of phylum Ascomycota was significantly different between the two study areas (p < 0.05). The percentage of OTUs numbers in seagrass sediment fungi shared in the two regions was 5.15%, and their relative abundance was 31.19%. In addition, there were significant differences between the Alpha diversity index (Shannon and Phylogenetic diversity) and Beta diversity of the fungal communities of seagrass sediments in the two study areas (p < 0.05). The FUNGuild functional prediction analysis revealed that the main fungal trophic types were undetermined (relative abundance 72.11% ~ 91.92%). The trophic types of the rest fungi were Symbiotroph, Saprotroph, and Pathotroph, and these three trophic types could be further divided into 41 functional guilds. Network analysis for fungal groups based on random matrix theory (RMT) showed that the fungi network structure of seagrass T. hemprichii sediment in Hainan Island was more complex, with higher average clustering coefficients, longer average path lengths, and higher densities. These fungal communities may be more sensitive to environmental change. While the fungal communities of seagrass T. hemprichii sediment in Xisha Islands were more modulized, the fungus belonging to Class Sordariomycetes was the key taxon in the molecular ecological network. This study provides essential primary data and theoretical support for further study on the structure and function of fungi in seagrass ecosystems, microbial resource mining, and ecological applications.

Key words: seagrass ecosystem, fungi community, FUNGuild, trophic type, molecular ecological network

LING Juan, LIANG Tongyin, YUE Weizhong, HUANG Xiaofang, SUN Cuici, ZHANG Jian, ZHANG Yuhang, ZHOU Weiguo, DONG Junde. Community structure, function, and molecular ecological network of fungi in the tropical seagrass Thalassia hemprichii sediment[J].Journal of Tropical Oceanography, 2023, 42(5): 64-75.

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序号	分组	编号	样品	采样地	海草种类组成	具体时间	经纬度
1	海南岛	XCT	泰来草沉积物	海南岛陵水新村湾海草特别保护区	泰来草海菖蒲	2015年6月1日	18°30′N, 109°59′E
2	海南岛	SYT	泰来草沉积物	海南岛三亚珊瑚礁自然保护区	泰来草	2015年6月1日	18°12′N, 109°30′E
3	西沙群岛	YXT	泰来草沉积物	西沙群岛永兴岛	泰来草、喜盐草、二药草	2015年5月28日	16°50′32″N, 112°20′41″E
4	西沙群岛	SDT	泰来草沉积物	西沙群岛石岛	泰来草、喜盐草	2015年5月28日	16°50'06″N, 112°22′10″E

研究区域	分子生态网络									随机网络
研究区域	节点	连线数	阈值	平均连通度	平均聚集系数	平均路径长度	模块性 (模块数)	密度	正相关	平均路径长度	平均聚集系数A	模块性
海南岛	61	515	0.950	16.885	0.589	2.308	0.172(4)	0.281	51.46%	1.862±0.024	0.524±0.016	0.111±0.009
西沙群岛	101	201	0.890	3.980	0.257	4.776	0.740(8)	0.040	57.71%	3.433 ±0.064	0.040 ± 0.013	0.458±0.013

Community structure, function, and molecular ecological network of fungi in the tropical seagrass Thalassia hemprichii sediment

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