西沙短指软珊瑚Sinularia flexibilis中具有肿瘤细胞毒活性的甾体化合物

doi:10.11978/2023038

Abstract

Abstract:

Thin layer chromatography, silica gel column chromatography, gel column chromatography and semi preparative high-performance liquid chromatography were used to study the chemical compositions of Sinularia flexibilis, a soft coral collected off the Xisha islands in the South China Sea. Eight steroids were identified from the soft coral S. flexibilis, 24-methylcholesta-5,24(28)-diene-3β,19-diol (1), (3β,23S)-ergosta-5,24(28)-diene-3,23-diol (2), 4α-methyl-3β,14β-dihydroxy-5α-ergost-24(28)-en-23-one (3), sinulasterol C (4), nephalsterol C (5), 24-methylene-cholesterol (6), 4α-methyl-3β, 8β-dihydroxy-5α-ergost-24(28)-en-23-one (7) and 4α-methyl-ergosterol-24-en-3β-ol (8) by the physical and chemical properties, extensive spectroscopic analysis and comparison with the reported data from literatures. The results of cytotoxic activity test showed compounds 2, 3 and 5 had strong inhibitory activity on K562 (human chronic myeloid leukemia cells), with IC₅₀ (half maximal inhibitory concentration) values of (5.48 ± 0.22) μmol·L^-1, (4.80 ± 0.11) μmol·L^-1 and (2.08 ± 0.11) μmol·L^-1, respectively.

Key words: soft coral, Sinularia flexibilis, chemical constituent, steroids, cytotoxicity

GAO Yaxin, WANG Hao, DAI Haofu, XIA Zhihui, ZENG Yanbo. Cytotoxic steroids from the soft coral Sinularia flexibilis collected off the Xisha[J].Journal of Tropical Oceanography, 2023, 42(5): 56-63.

Figures/Tables 3

References 26

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化合物	(IC₅₀ ± SD)/(μmol·L^-1)^a
化合物	K562	BEL-7402	SGC-7901	A549	Hela
1	-	-	-	-	-
2	5.48 ± 0.22	26.76 ± 0.10	19.40 ± 0.27	43.69 ± 0.60	44.44 ± 0.65
3	4.80 ± 0.11	29.64 ± 0.52	23.54 ± 0.68	36.44 ± 0.29	42.00 ± 1.17
4	-	-	-	-	-
5	2.08 ± 0.11	9.09 ± 0.08	16.57 ± 0.04	28.69 ± 0.25	20.10 ± 0.19
6	-	-	-	-	-
7	-	-	-	-	-
8	-	-	-	-	-
顺铂^b	3.08 ± 0.05	4.02 ± 0.08	4.11 ± 0.02	1.93 ± 0.02	11.29 ± 0.15

Cytotoxic steroids from the soft coral Sinularia flexibilis collected off the Xisha

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