胡氏凯伦藻(Karenia hui)对卤虫(Artemia salina)的急性毒性及溶血活性研究

doi:10.11978/2024193

Abstract

Abstract:

Karenia harmful algal blooms occur frequently worldwide, attracting extensive attention from countries around the globe. Karenia hui, identified as a new species of the genus Karenia in 2024, has been found to be lethal to Oryzias melastigma. Research on this species is currently quite limited. This study investigated its growth characteristics, acute toxicity on Artemia salina, and hemolytic activity at different growth stages. This study aims to reveal the growth curve of K. hui, its toxic effects, and changes in hemolytic activity. The results indicated that K. hui had a unique growth cycle. After the initial exponential growth period and the stable period, it entered the exponential growth period and the stable period again. Karenia hui possessed acute toxicity to Artemia salina, with the death rate of Artemia salina being positively correlated with the density of the algae and increasing with exposure time. In the study of the acute toxicity of K. hui at different growth stages to Artemia salina, the toxicity was the highest on day 23 of the initial stationary phase. The specific death rate of Artemia salina reached its peak at (0.22±0.03)h^-1 within 24 hours, and the death rate after 72 hours reached (98.33±4.08)%. The hemolytic activity of K. hui increased during the first exponential growth phase and reached its peak at the end of this phase (14.43×10^-6HU·cells^-1). It began to decline during the first stationary phase, and remained low during the second exponential growth phase and stationary phase (0.31×10^-6HU·cells^-1). The results of this study can provide a foundation for assessing and mitigating the potential ecological risks posed by K. hui blooms.

Key words: Karenia, Karenia hui, acute toxicity, hemolytic activity

CLC Number:

CHEN Shibing, HUANG Bozhu, XIE Xuedong, ZHAO Jinping, LYU Songhui, CEN Jingyi. Study of acute toxicity of Karenia hui on Artemia salina and its hemolytic activity[J].Journal of Tropical Oceanography, 2025, 44(1): 162-171.

Figures/Tables 7

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时间段	比死亡速率(h^-1)
时间段	低密度组	中密度组	高密度组	对照组	饥饿组
0—24h	0.11±0.03^a	0.19±0.04^b	0.26±0.02^c	0.03±0.02^d	0.03±0.02^d
24—48h	0.10±0.02^a	0.11±0.04^a	0.13±0.03^a	0.01±0.02^b	0.04±0.03^b
48—72h	0.12±0.04^a	0.08±0.04^a	0.02±0.02^b	0.02±0.02^b	0.01±0.01^b
72—96h	0.08±0.03^a	0.03±0.03^bc	0.00±0.00^b	0.04±0.03^c	0.02±0.02^bc

时间段	密度组别	不同培养天数下的比死亡速率(h^-1)
时间段	密度组别	5d	10d	15d	23d	31d	39d
0—24h	低	0.13±0.03^a	0.13±0.04^a	0.15±0.04^a	0.19±0.02^b	0.12±0.03^a	0.13±0.05^a
0—24h	高	0.18±0.02^ab	0.17±0.03^ab	0.20±0.03^bc	0.22±0.03^c	0.15±0.03^a	0.17±0.03^ab
24—48h	低	0.11±0.04^a	0.13±0.06^a	0.13±0.07^a	0.10±0.04^a	0.15±0.03^a	0.14±0.06^a
24—48h	高	0.12±0.04^a	0.13±0.03^a	0.11±0.07^a	0.10±0.06^a	0.15±0.03^a	0.13±0.05^a
48—72h	低	0.06±0.06^ab	0.06±0.04^a	0.10±0.02^abc	0.10±0.04^abc	0.11±0.03^bc	0.13±0.05^c
48—72h	高	0.06±0.04^a	0.07±0.04^a	0.08±0.07^a	0.08±0.07^a	0.08±0.06^a	0.09±0.03^a
72—96h	低	0.10±0.07^a	0.10±0.03^a	0.03±0.04^b	0.01±0.02^b	0.04±0.04^b	0.03±0.02^b
72—96h	高	0.04±0.03^a	0.04±0.04^a	0.02±0.02^a	0.01±0.02^c	0.03±0.03^a	0.02±0.02^a

Study of acute toxicity of Karenia hui on Artemia salina and its hemolytic activity

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