羊栖菜共附生真菌Aspergillus sp. GXIMD 02045的次级代谢产物研究

doi:10.11978/2024038

Abstract

Abstract:

To explore the diversity of secondary metabolites of fungus Aspergillus sp. GXIMD 02045 from Sargassum fusiforme, the secondary metabolites were separated with various chromatographic techniques. Chemical structures were identified using mass spectroscopy, nuclear magnetic resonance spectroscopy, and optical rotation data with comparison of literature reported ones. 15 compounds were isolated from Aspergillus sp. GXIMD 02045, and identified as (3S)-6-hydroxy-8-methoxy-3, 5-dimethylisochroman (1), (3S)-6-hydroxy-8-methoxy-3-methylisochroman (2), (3S, 4R)-6-hydroxy-8-methoxy-3, 5-dimethylisochroman (3), (1S, 3R)-3, 7-dimethyl-1, 8-dihydroxy-6-methoxyisochroman (4), (S)-6-hydroxy-7-methoxy-3, 5-dimethylisochroman-1-one (5), anserinone A (6), anserinone B (7), (+)-formylanserinone B (8), 4-hydroxyphenylacetic acid (9), p-hydroxyphenylacetic acid methyl ester (10), Waol A (11), (S)-7-methoxy-2, 5-dimethyl-2, 3-dihydrobenzofuran-6-ol (12), 6-methylcurvulinic acid methyl ester (13), 4-hydroxy-3, 6-dimethyl-2-pyrone (14), trans-ferulic acid (15). Antibacterial activity of secondary metabolites from A. sp. GXIMD02045 was tested, which showed that compounds 6−8 displayed inhibitory activity against methicillin-resistant Staphylococcus aureus, and the minimum inhibitory concentration (MIC) values were ranging from 3.91 μg·mL^-1 to 7.81 μg·mL^-1. Compounds 6−8 and 11 showed inhibitory activity against Bacillus subtilis, and the MIC values were ranging from 7.81 μg·mL^-1 to 15.62 μg·mL^-1. Compounds 4, 6−8, 10 and 11 showed inhibitory activity against Pseudomona aeruginosa, and the MIC values were ranging from 1.95 μg·mL^-1 to 125.00 μg·mL^-1. This study provides a theoretical basis for the research on development and utilization of mangrove Sargassum fusiforme and its symbiotic fungus.

Key words: Sargassum fusiforme, Aspergillus fungus, secondary metabolites, antibacterial activity

CLC Number:

O629

KONG Weiqi, JIN Xin, LIU Yonghong, GAO Chenghai, CHEN Xianqiang. Studies on secondary metabolites of Sargassum fusiforme derived fungus Aspergillus sp. GXIMD 02045[J].Journal of Tropical Oceanography, 2025, 44(1): 154-161.

Figures/Tables 2

References 25

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Studies on secondary metabolites of Sargassum fusiforme derived fungus Aspergillus sp. GXIMD 02045

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化合物	MIC-耐甲氧西林金黄葡萄球菌	MIC-枯草芽孢杆菌	MIC-铜绿假单胞菌
4	-	-	62.50
6	7.81	15.62	3.91
7	3.91	15.62	3.91
8	7.81	7.81	3.91
10	-	-	125.00
11	-	7.81	1.95
阳性药(环丙沙星)	0.78	0.05	0.10

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