纳氏试剂光度法快速测定海水中氨氮的方法优化

doi:10.11978/2024237

Abstract

Abstract:

The complex salt components in seawater or other saline water may cause turbidity when measuring ammonia nitrogen by Nessler’s reagent colorimetric method. In this study, interference from Ca, Mg, Fe, Mn and other ions in seawater was eliminated by precisely matching sample salinity with the dosage of potassium sodium tartrate as a masking agent. The Nessler reagent spectrophotometery for rapid ammonia nitrogen determination was thus optimized. The results indicate that 1% potassium sodium tartrate solution is suitable for ammonia nitrogen determination in samples with salinity below 17.3‰, while 2% potassium sodium tartrate solution can be used for samples with salinity ranging from 4.5‰ to 34.1‰ under experimental conditions. The method’s detection range was 0.1-8.0 mg·L^-1, with a detection limit of 0.03 mg·L^-1. The spiked recovery rates of samples ranged from 96% to 105%, and the standard deviation of parallel samples was less than 3.94%. The method offers simple operation, a wide linear range, good sensitivity and accuracy, and broad applicability.

Key words: Nessler’s reagent, spectrophotometry, seawater samples, ammonia nitrogen, potassium sodium tartrate

CLC Number:

P761.1

HUANG Chunhui, WEN Chang, XIA Qing, HUANG Weiwei, WANG Xiangguang, YANG Fei. Optimization of rapid ammonia nitrogen determination in seawater using Nessler reagent spectrophotometery[J].Journal of Tropical Oceanography, 2025, 44(5): 201-208.

Figures/Tables 9

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References 19

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样品盐度/‰	不同酒石酸钾钠溶液浓度下的吸光值
样品盐度/‰	1%	2%	3%	4%
34.1	-	0.487±0.004	0.516±0.006	-
25.7	-	0.502±0.005	0.509±0.005	-
17.3	0.486±0.008	0.494±0.001	0.510±0.004	-
9.0	0.473±0.005	0.496±0.002	-	-
4.5	0.496±0.002	0.586±0.009	-	-
2.3	0.496±0.002	-	-	-

样品盐度/‰	纳氏试剂体积/mL
样品盐度/‰	0.10	0.20	0.30	0.40	0.50	0.60
34.1	-	-	-	-	-	-
25.7	-	-	-	-	-	0.407±0.002
17.3	-	-	0.394±0.004	0.398±0.001	0.405±0.002	0.420±0.002
9.0	0.341±0.002	0.391±0.004	0.409±0.003	0.430±0.004	0.429±0.005	0.409±0.001
4.5	0.367±0.003	0.391±0.003	0.415±0.003	0.419±0.003	0.423±0.004	0.426±0.004
2.3	0.358±0.002	0.391±0.004	0.414±0.004	0.419±0.003	0.423±0.003	0.426±0.003

样品盐度/‰	纳氏试剂体积/mL
样品盐度/‰	0.10	0.20	0.30	0.40	0.50	0.60
34.1	-	-	-	0.405±0.002	0.441±0.002	0.444±0.001
25.7	-	-	0.478±0.004	0.495±0.001	0.491±0.001	0.498±0.002
17.3	-	0.445±0.006	0.478±0.006	0.485±0.001	0.494±0.002	0.494±0.002
9.0	-	0.473±0.005	0.498±0.003	0.494±0.002	0.498±0.002	0.514±0.003
4.5	-	0.497±0.006	0.551±0.007	0.521±0.004	0.542±0.004	0.529±0.005
2.3	-	-	-	-	-	-

样品盐度/‰	不同酒石酸钾钠溶液浓度下的吸光值
样品盐度/‰	1%	2%	3%	4%
34.1	-	0.640±0.004	0.652±0.006	-
25.7	-	0.645±0.005	0.614±0.004	-

样品盐度/‰	20%氢氧化钠溶液使用量/mL
	0.05		0.10		0.20		0.30		0.40
	0mg·L^-1	4mg·L^-1	0mg·L^-1	4mg·L^-1	0mg·L^-1	4mg·L^-1	0mg·L^-1	4mg·L^-1	0mg·L^-1	4mg·L^-1
34.1	-	-	-	-	-	-	0.067±0.002	0.499±0.002	0.055±0.002	-
27.4	-	-	-	-	0.052±0.002	0.482±0.002	0.054±0.001	0.532±0.003	0.050±0.001	-
20.6	-	-	0.066±0.001	0.489±0.005	0.053±0.002	0.499±0.003	0.050±0.001	0.553±0.003	0.052±0.002	-

Optimization of rapid ammonia nitrogen determination in seawater using Nessler reagent spectrophotometery

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样品	测定值/(mg·L^-1)	标准偏差	加标量/(mg·L^-1)	测得总量/(mg·L^-1)	加标回收率/%
养殖尾水1	2.74	0.030	2.58	5.35	100.80
养殖尾水2	1.94	0.019	1.90	3.79	97.06
人工海水	2.07	0.019	2.00	4.17	105.10
感潮湖水	1.16	0.025	2.00	3.18	96.22
海洋牧场	0.70	0.019	1.00	1.67	96.89

样品名称	氨氮含量/(mg·L^-1)	测定值/(mg·L^-1)	标准偏差	相对误差/%
34.1‰无氨海水1	0.50	0.48	0.020	-4.20
34.1‰无氨海水2	3.00	3.05	0.029	1.78
34.1‰无氨海水3	6.00	6.07	0.026	1.11
32.7‰人工海水1	0.50	0.54	0.011	8.07
32.7‰人工海水2	3.00	3.14	0.039	4.58
32.7‰人工海水3	6.00	6.13	0.037	2.14
17.8‰无氨海水	5.00	5.02	0.028	0.79
8.9‰无氨海水	2.00	2.09	0.030	1.46