网采镜检与全息粒子成像综合分析贻贝养殖区浮游植物群落特征

doi:10.11978/2025051

Abstract

Abstract:

This study integrated net sampling with microscopic examination and an underwater digital holographic particle imaging system (LISST Holo2) to analyze the diurnal dynamics and environmental drivers of phytoplankton communities in suspended mussel raft aquaculture areas near Gouqi Island, Zhejiang Province. Field data collected in August 2023 identified 75 phytoplankton species across 5 phyla and 38 genera, with Noctiluca scintillans and Rhizosolenia styliformis as dominant species, coinciding with a Noctiluca scintillans red tide outbreak. The aquaculture layer exhibited fewer phytoplankton species and lower abundance compared to the entire water column, but demonstrated higher co-occurrence network complexity, greater community stability, and stronger inter-species interactions. Tidal cycles significantly influenced phytoplankton distribution in the entire water column [GAM (generalized additive models) explained 57.4% of variation], while temporal factors dominated abundance fluctuations in the aquaculture layer (P=0.016). The LISST Holo2 detected 34 genera from 3 phyla of phytoplankton, accounting for 89.4% of the taxa identified by net sampling and microscopy. For large-sized phytoplankton (e.g., Noctiluca scintillans), LISST Holo2 showed high consistency with traditional net sampling and microscopy results, with a dominance curve correlation coefficient of R²>0.8. Compared to conventional methods, LISST Holo2 addressed challenges such as laborious sample preservation, delayed data acquisition, and particle size measurement difficulties. This study validates its reliability for large-sized algal monitoring and underscores the roles of mussel filtration pressure and raft-induced hydrodynamic effects in reshaping phytoplankton communities, providing critical insights for optimizing aquaculture practices and advancing marine ecosystem monitoring technologies.

Key words: LISST Holo2, suspended mussel farms, phytoplankton community, environment factors, phytoplankton co-occurrence network

CLC Number:

Q948.8

YANG Tianwei, LIN Jun, JIAO Junpeng, WU Yue. Integrated analysis of phytoplankton community structure in mussel aquaculture areas using net sampling with microscopy and LISST Holo2[J].Journal of Tropical Oceanography, 2025, 44(6): 91-107.

Figures/Tables 17

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References 45

[1]	毕远溥, 董婧, 蒋双, 等, 2002. 小窑湾双壳贝类筏式养殖对海域环境的影响[J]. 应用与环境生物学报, 8(3): 270-275.
	BI YUANPU, DONG JING, JIANG SHUANG, et al, 2002. Effects of bivalve raft cultivation on environment characteristics of the Xiaoyao Bay[J]. Chinese Journal of Applied and Environmental Biology, 8(3): 270-275 (in Chinese with English abstract).
[2]	陈丹丹, 庞巧珠, 涂志刚, 等, 2021. 后水湾深水网箱养殖区浮游植物群落季节变化及其与环境因子的关系[J]. 海洋环境科学, 40(1): 73-80.
	CHEN DANDAN, PANG QIAOZHU, TU ZHIGANG, et al, 2021. Seasonal variation of phytoplankton community and its relationship with environmental factors in deep-water cage aquaculture area of Houshui Bay[J]. Marine Environmental Science, 40(1): 73-80 (in Chinese with English abstract).
[3]	陈奕帆, 王君玥, 王英豪, 等, 2024. 典型赤潮生物夜光藻研究进展[J]. 大连海洋大学学报, 39(6): 1075-1086.
	CHEN YIFAN, WANG JUNYUE, WANG YINGHAO, et al, 2024. Research progress on typical red tide organism Noctiluca scintillans: a review[J]. Journal of Dalian Ocean University, 39(6): 1075-1086 (in Chinese with English abstract).
[4]	代鲁平, 李超伦, 王世伟, 等, 2016. 基于ZooScan图像技术的南黄海夏季浮游动物群落结构分析[J]. 海洋与湖沼, 47(4): 764-773.
	DAI LUPING, LI CHAOLUN, WANG SHIWEI, et al, 2016. Analysis of community structure of zooplankton in south Yellow Sea in summer with zooscan[J]. Oceanologia et Limnologia Sinica, 47(4): 764-773 (in Chinese with English abstract).
[5]	段晓萌, 秦华伟, 马浩阳, 等, 2023. 黄河口及莱州湾海域磷的时空分布及浮游生物对低磷胁迫的响应[J]. 中国海洋大学学报(自然科学版), 53(11): 87-98.
	DUAN XIAOMENG, QIN HUAWEI, MA HAOYANG, et al, 2023. Temporospatial distribution of phosphorus and response of phytoplankton to low phosphorus stress in the Yellow River Estuary and Laizhou Bay[J]. Periodical of Ocean University of China, 53(11): 87-98 (in Chinese with English abstract).
[6]	凡仁福, 魏皓, 赵亮, 2015. 高生产力区LISST-100和OBS对悬浮颗粒物测量的比较研究[J]. 海洋科学进展, 33(4): 492-500.
	FAN RENFU, WEI HAO, ZHAO LIANG, 2015. The comparison between LISST-100 and OBS observation on the suspended particulate material in the high-production area[J]. Advances in Marine Science, 33(4): 492-500 (in Chinese with English abstract).
[7]	关莹莹, 林军, 焦俊鹏, 等, 2022. 高滤食压力下贻贝筏式养殖场及周边海域浮游植物群落特征[J]. 海洋环境科学, 41(4): 543-553.
	GUAN YINGYING, LIN JUN, JIAO JUNPENG, et al, 2022. The characteristics of phytoplankton community of mussel raft farms and surrounding waters under high filtration pressure[J]. Marine Environmental Science, 41(4): 543-553 (in Chinese with English abstract).
[8]	关莹莹, 2021. 规模化贻贝养殖海域浮游植物群落结构及其环境影响因子研究[D]. 上海: 上海海洋大学.
	GUAN YINGYING, 2021. Phytoplankton community in the large-scale mussel culture sea area and its relationship with environmental factors[D]. Shanghai: Shanghai Ocean University. (in Chinese with English abstract)
[9]	霍笑康, 王永刚, 周灵同, 等, 2024. 结合生态网络解析白洋淀浮游生物生态位和种间联结性特征[J]. 环境科学, 45(9): 5298-5307.
	HUO XIAOKANG, WANG YONGGANG, ZHOU LINGTONG, et al, 2024. Characterization of the ecological niche and interspecific connectivity of plankton in Baiyangdian Lake by combining ecological networks[J]. Environmental Science, 45(9): 5298-5307 (in Chinese with English abstract).
[10]	焦念志, 杨燕辉, MANN E, 等, 1998. 我国东海发现原绿球藻的大量存在[J]. 科学通报, 43(6): 654 (in Chinese).
[11]	李红飞, 林森杰, 2019. 南海浮游植物生态学研究进展[J]. 厦门大学学报(自然科学版), 58(1): 1-10.
	LI HONGFEI, LIN SENJIE, 2019. Research progresses of phytoplankton ecology in the South China Sea[J]. Journal of Xiamen University (Natural Science), 58(1): 1-10 (in Chinese with English abstract).
[12]	李立群, 王艳, 王彪, 等, 2024. 2009—2021年夏季长江口海域浮游生物群落结构时空分布特征及其影响因素研究[J]. 环境科学研究, 37(2): 233-245.
	LI LIQUN, WANG YAN, WANG BIAO, et al, 2024. Spatiotemporal distribution of plankton community structure in the Yangtze River Estuary in the summer of 2009-2021 and its influencing factors[J]. Research of Environmental Sciences, 37(2): 233-245 (in Chinese with English abstract).
[13]	李斯远, 2022. 厚壳贻贝的摄食选择偏好及其食物识别机制研究[D]. 舟山: 浙江海洋大学.
	LI SIYUAN, 2022. Feeding preference and food recognition mechanism of Mytilus coruscus[D]. Zhoushan: Zhejiang Ocean University (in Chinese with English abstract).
[14]	梁文钊, 唐丹玲, 2017. 南海西部夏季表层浮游植物粒径结构分布特征分析[J]. 热带海洋学报, 36(4): 93-101. doi: 10.11978/2016104
	LIANG WENZHAO, TANG DANLING, 2017. Distribution characteristics of phytoplankton size structure in the western South China Sea in summer[J]. Journal of Tropical Oceanography, 36(4): 93-101 (in Chinese with English abstract). doi: 10.11978/2016104
[15]	陆斗定, 张志道, 朱根海, 等, 1994. 浙江近海夜光藻的分布及其生态学特点[J]. 东海海洋, 12(3): 62-69.
	LU DOUDING, ZHANG ZHIDAO, ZHU GENHAI, et al, 1994. Distributive Pattern of Noctiluca scintillans in the Zhejiang coastal water area[J]. Journal of Marine Sciences, 12(3): 62-69.
[16]	施玉珍, 张才学, 张际标, 等, 2017. 水东湾海域浮游植物潮汐分布特征及其与环境因子的关系[J]. 生态学报, 37(18): 5981-5992.
	SHI YUZHEN, ZHANG CAIXUE, ZHANG JIBIAO, et al, 2017. Effect of tidal changes and major environmental factors on the distribution of phytoplankton in Shuidong Bay[J]. Acta Ecologica Sinica, 37(18): 5981-5992 (in Chinese with English abstract).
[17]	孙晓霞, 郭术津, 刘梦坛, 等, 2021. 印太交汇区浮游植物和浮游动物生态学研究进展[J]. 海洋与湖沼, 52(2): 323-331.
	SUN XIAOXIA, GUO SHUJIN, LIU MENGTAN, et al, 2021. Research progress on phytoplankton and zooplankton ecology in indo-Pacific convergence region[J]. Oceanologia et Limnologia Sinica, 52(2): 323-331 (in Chinese with English abstract).
[18]	孙东, 赵冬至, 文世勇, 等, 2010. 夜光藻赤潮与环境因子关系的模糊分析[J]. 海洋环境科学, 29(1): 70-75.
	SUN DONG, ZHAO DONGZHI, WEN SHIYONG, et al, 2010. Fuzzy analysis on relationship between Noctiluca scintillans blooms and environmental factors[J]. Marine Environmental Science, 29(1): 70-75 (in Chinese with English abstract).
[19]	宋书群, 孙军, 俞志明, 2009. 长江口及其邻近水域叶绿素a的垂直格局及成因初析[J]. 植物生态学报, 33(2): 369-379. doi: 10.3773/j.issn.1005-264x.2009.02.015
	SONG SHUQUN, SUN JUN, YU ZHIMING, 2009. Vertical pattern of chlorophyll a in the Yangtze River estuary and its adjacent waters[J]. Chinese Journal of Plant Ecology, 33(2): 369-379 (in Chinese with English abstract). doi: 10.3773/j.issn.1005-264x.2009.02.015
[20]	唐启升, 蒋增杰, 毛玉泽, 2022. 渔业碳汇与碳汇渔业定义及其相关问题的辨析[J]. 渔业科学进展, 43(5): 1-7.
	TANG QISHENG, JIANG ZENGJIE, MAO YUZE, 2022. Clarification on the definitions and its relevant issues of fisheries carbon sink and carbon sink fisheries[J]. Progress in Fishery Sciences, 43(5): 1-7 (in Chinese with English abstract).
[21]	唐紫琦, 宋敏杰, 王馨, 等, 2024. 2022年南黄海绿潮分布与浮游生物群落关系初探[J]. 海洋科学, 48(3): 26-41.
	TANG ZIQI, SONG MINJIE, WANG XIN, et al, 2024. Relationship between green tide distribution and plankton community in the South Yellow Sea in 2022[J]. Marine Sciences, 48(3): 26-41 (in Chinese with English abstract).
[22]	谭益国, 钟威, 林军, 等, 2024. 浮筏式贻贝养殖设施阻流效应的数值模拟[J/OL]. 水产学报: 1-13. (2024-02-19) [2025-04-04]. https://kns.cnki.net/kcms/detail/31.1283.S.20240207.1550.002.html
	TAN YIGUO, ZHONG WEI, LIN JUN, et al, 2024. Numerical simulation of flow resistance effect around the suspended mussel aquaculture[J/OL]. Journal of Fisheries of China: 1-13. (2024-02-19) [2025-04-04]. https://kns.cnki.net/kcms/detail/31.1283.S.20240207.1550.002.htmlin Chinese with English abstract).
[23]	陶宗娅, 邹琦, 1999. 植物光合作用光抑制分子机理及其光保护机制[J]. 西南农业学报, 12(S2): 9-18.
	TAO ZONGYA, ZOU QI, 1999. Molecular mechanisms of photodamage and protective mechanism against photoinhibition and photodamage in photosynthetic apparatus of higher plant[J]. Southwest China Journal of Agricultural Sciences, 12(S2): 9-18.
[24]	王旭, 赵旭, 章守宇, 等, 2015. 枸杞岛贻贝养殖水域碳氮磷分布格局[J]. 水产学报, 39(11): 1650-1664.
	WANG XU, ZHAO XU, ZHANG SHOUYU, et al, 2015. Distribution pattern of dissolved carbon, nitrogen and phosphorus in mussel culture areas of Gouqi Island[J]. Journal of Fisheries of China, 39(11): 1650-1664 (in Chinese with English abstract).
[25]	吴晓凡, 汪振华, 章守宇, 等, 2024. 大规模筏式贻贝养殖区浮游植物群落结构昼夜变化特征[J]. 上海海洋大学学报, 33(5): 1211-1222.
	WU XIAOFAN, WANG ZHENHUA, ZHANG SHOUYU, et al, 2024. Diurnal variation of phytoplankton community structure in a large-scale raft mussel aquaculture area[J]. Journal of Shanghai Ocean University, 33(5): 1211-1222 (in Chinese with English abstract).
[26]	温英, 林军, 杨冠林, 等, 2022. 贻贝浮筏养殖设施水动力效应及附生海藻碎屑输运的数值模拟[J]. 上海海洋大学学报, 31(6): 1549-1561.
	WEN YING, LIN JUN, YANG GUANLIN, et al, 2022. Study on hydrodynamic effect of suspended mussel aquaculture facilities and detritus transportation of epiphytic seaweed[J]. Journal of Shanghai Ocean University, 31(6): 1549-1561 (in Chinese with English abstract).
[27]	徐亚军, 赵亮, 原野, 2016. 基于声学仪器与粒径分析仪研究东海浮游动物昼夜垂直迁移过程[J]. 海洋学报, 38(8): 123-130.
	XU YAJUN, ZHAO LIANG, YUAN YE, 2016. The diel vertical migration of zooplankton observed by an acoustic Doppler current profiler and particle size analyzer in the East China Sea[J]. Haiyang Xuebao, 38(8): 123-130 (in Chinese with English abstract).
[28]	徐兆礼, 高文烨, 2004. 东海赤潮高发区夜光藻数量分布与环境关系的研究[C]// 中国水产学会. 中国水产学会第五届青年学术年会摘要集, 上海: 中国水产学会: 135.
	XU ZHAO LI, GAO WEN YE, 2004. Study on the relationship between the distribution of Noctiluca scintillans abundance and environmental factor at the frequent HAB in the East China Sea[C]// China Society of Fisheries. Abstracts of the 5th youth academic annual conference of the China Society of Fisheries. Shanghai: China Society of Fisheries:135 (in Chinese with English abstract).
[29]	杨冠林, 2022. 典型贻贝浮筏式养殖海域物理—生态—贝类生长耦合数值模型的构建及应用[D]. 上海: 上海海洋大学.
	YANG GUANLIN, 2022. Construction and application of a coupled bio-physics with individual-based shellfish ecophysiology model for the suspended mussel aquaculture farm[D]. Shanghai: Shanghai Ocean University (in Chinese with English abstract).
[30]	岳新利, 张梦柯, 谷利德, 等, 2023. 福建平潭外海域夜光藻赤潮期营养元素和溶解态痕量金属动态与响应[J]. 厦门大学学报(自然科学版), 62(3): 375-384.
	YUE XINLI, ZHANG MENGKE, GULI DE, et al, 2023. Dynamics of nutrients and dissolved trace metals during an algal bloom of Noctiluca scintillans in the waters off the Pingtan Island, Fujian[J]. Journal of Xiamen University (Natural Science), 62(3): 375-384 (in Chinese with English abstract).
[31]	赵文, 2005. 水生生物学[M]. 北京: 中国农业出版社 (in Chinese).
[32]	周曦杰, 2014. 枸杞岛典型生境螺贝类代表种—角蝾螺、紫贻贝摄食生态初步研究[D]. 上海: 上海海洋大学.
	ZHOU XIJIE, 2014. Preliminary study on feeding behaviour of two representative species in typical habitats (seaweedbed & musselfarm) in Gouqi Island: the gastropod, Turbo cornutus Solander; the mussel, Mytilus edulis[D]. Shanghai: Shanghai Ocean University (in Chinese with English abstract).
[33]	ARA K, NAKAMURA S, TAKAHASHI R, et al, 2013. Seasonal variability of the red tide-forming heterotrophic dinoflagellate Noctiluca scintillans in the neritic area of Sagami Bay, Japan: its role in the nutrient-environment and aquatic ecosystem[J]. Plankton and Benthos Research, 8(1): 9-30. doi: 10.3800/pbr.8.9
[34]	ARRIETA J, JEANNERET R, ROIG P, et al, 2020. On the fate of sinking diatoms: the transport of active buoyancy-regulating cells in the ocean[J]. Philosophical Transactions Series A, Mathematical, Physical, and Engineering Sciences, 378(2179): 20190529.
[35]	CHISHOLM S W, OLSON R J, ZETTLER E R, et al, 1988. A novel free-living prochlorophyte abundant in the oceanic euphotic zone[J]. Nature, 334(6180): 340-343. doi: 10.1038/334340a0
[36]	DHARANI G, ABDUL NAZAR A K, KANAGU L, et al, 2004. On the recurrence of Noctiluca scintillans bloom in Minnie Bay, Port Blair: Impact on water quality and bioactivity of extracts[J]. Current Science, 87(7): 990-994.
[37]	KIM Y H, LEE H J, et al, 2015. Under-ice measurements of suspended particulate matters using ADCP and LISST-Holo[J]. Ocean Science Journal, 50(1): 97-108. doi: 10.1007/s12601-015-0008-2
[38]	JAGO C, ROBINS P, HOWLETT E, et al, 2024. Trapping and bypassing of suspended particulate matter, particulate nutrients and faecal indicator organisms in the river-estuary transition zone of a shallow macrotidal estuary[J]. Science of the Total Environment, 917: 170343. doi: 10.1016/j.scitotenv.2024.170343
[39]	JUSTIĆ D, RABALAIS N N, TURNER R E, 1995. Stoichiometric nutrient balance and origin of coastal eutrophication[J]. Marine Pollution Bulletin, 30(1): 41-46. doi: 10.1016/0025-326X(94)00105-I
[40]	NELSON D M, BRZEZINSKI M A, 1990. Kinetics of silicic acid uptake by natural diatom assemblages in two Gulf Stream warm-core rings[J]. Marine Ecology Progress Series, 62: 283-292. doi: 10.3354/meps062283
[41]	PIELOU E C, 1969. An Introduction to Mathematical Ecology[M]. New York: Wiley-Interscience: 1-286.
[42]	SHANNON C E, 2001. A mathematical theory of communication[J]. ACM SIGMOBILE Mobile Computing and Communications Review, 5(1): 3-55.
[43]	TER BRAAK C J F, SMILAUER P, 2012. Canoco reference manual and user's guide: software for ordination (Version 5. 0)[M]. Ithaca: Microcomputer Power.
[44]	ULANOWICZ R E, 2001. Information theory in ecology[J]. Computers & Chemistry, 25(4): 393-399.
[45]	ZHONG WEI, LIN JUN, ZOU QINGPING, et al, 2022. Hydrodynamic effects of large-scale suspended mussel farms: Field observations and numerical simulations[J]. Frontiers in Marine Science, 9: 973155. doi: 10.3389/fmars.2022.973155

	多样性指数	丰富度指数	均匀度指数
养殖水层	2.87	4.58	0.50
全水层	3.05	5.85	0.49

编号	优势种	优势度		粒径大小	形态特征
编号	优势种	贻贝养殖层	全水层	粒径大小	形态特征
A1	中肋骨条藻(Skeletonema costatum)	/	0.04	直径6~22μm	单个细胞近似球形, 链长2~8个细胞
A2	笔尖形根管藻(Rhizosolenia styliformis)	0.35	0.36	直径5~15μm,长200~500μm	细胞呈长圆柱形, 末端尖锐
A3	洛氏角毛藻(Chaetoceros lorenzianus)	/	0.04	长约20~50μm, 宽约10~30μm	细胞呈圆柱形或椭圆形通常形成短链, 由 2~6个细胞组成
A4	尖刺伪菱形藻(Pseudo-nitzschia pungens)	0.03	/	宽2~5μm, 长70~130μm	细长针形
A5	柔软伪菱形藻(Pseudo-nitzschia delicatissima)	0.06	0.05	宽1.5~3μm, 长30~80μm	较尖刺伪菱形藻更纤细
A6	佛氏海线藻(Thalassionema frauenfeldii	/	0.02	宽2~5μm, 长20~60μm	线状或短棒状, 常形成星形群体
B1	夜光藻(Noctiluca scintillans)	0.31	0.29	直径200~2000μm	球形或肾形

Integrated analysis of phytoplankton community structure in mussel aquaculture areas using net sampling with microscopy and LISST Holo2

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