中国南海Bukholderia sp. DA2菌株降解邻苯二甲酸二甲酯研究

doi:10.11978/j.issn.1009-5470.2010.04.071

Journal of Tropical Oceanography ›› 2010, Vol. 29 ›› Issue (4): 71-75.doi: 10.11978/j.issn.1009-5470.2010.04.071cstr: 32234.14.j.issn.1009-5470.2010.04.071

• Marine biology • Previous Articles Next Articles

Biodegradation of dimethyl phthalate by Burkholderia sp. DA2 isolated from marine sediments in the South China Sea

WANG Ya-li1,2, YIN Bo1, HONG Yi-guo1, GU Ji-dong1

1. Key laboratory of Tropical Marine Environment Dynamic, South China Sea Institute of Oceanography, CAS, Guangzhou 510301, China; 2. Guangdong Museum, Guangzhou 510110, China

Received:2008-04-10 Revised:2008-06-06 Online:2010-07-31 Published:2010-07-29
About author:王亚丽(1978—), 女, 山东省莱州市人, 博士研究生, 专业方向为环境与分子微生物学。
Supported by:
广东省自然科学基金博士启动项目(06301430); 国家自然科学基金(30800032)

Abstract

Abstract:

Burkholderia sp. DA2 strain isolated from marine sediments in the South China Sea was capable of utilizing dimethyl phthalate (DMP) as the sole source of carbon and energy for biodegradation of DMP, DMT (dimethyl phthalate) and DMI (dimethyl isophthalate). During the transformation of DMP (400mg·L-1), its corresponding intermediates were identified as mono-methyl phthalate (MMP) and phthalate acid (PA) by HPLC analysis. By the 9th day, the DMP concentration was undetectable in the culture, corresponding to a DMP decrease; MMP accumulated to 38.9 mg/L during eight days, at the same time amount of PA was also produced during the first 12 days of degradation; this PA was completely degraded after 15 days. During the transformation of DMT (100mg·L-1), 80% DMT was transferred into MMT in one day, but the MMT could not degraded rapidly and accumulated to 80.2mg·L-1, and completely mineralized during eight days. During the DMI (200mg·L-1) degradation, the DMI was transferred into MMI after six days, but the MMI could not further mineralize. Maybe the compounds chemical structures infect the activity of the enzyme degradation. The biodegradation pathway of DMP, DMT and DMI was proposed as DMP\DMT\DMI→MMP\MMT\MMI→PA\TA\IA→ ... →CO2+H2O.

Key words: DMP, DMI, DMT, biodegradation, marine microorganism

WANG Ya-li,YIN Bo,HONG Yi-guo,GU Ji-dong. Biodegradation of dimethyl phthalate by Burkholderia sp. DA2 isolated from marine sediments in the South China Sea[J].Journal of Tropical Oceanography, 2010, 29(4): 71-75.

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Biodegradation of dimethyl phthalate by Burkholderia sp. DA2 isolated from marine sediments in the South China Sea

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