江蓠总DNA提取及其PCR扩增方法的建立

doi:10.11978/j.issn.1009-5470.2010.06.098

Journal of Tropical Oceanography ›› 2010, Vol. 29 ›› Issue (6): 98-103.doi: 10.11978/j.issn.1009-5470.2010.06.098cstr: 32234.14.j.issn.1009-5470.2010.06.098

• Marine biology • Previous Articles Next Articles

DNA isolation and PCR amplified in Gracilaria

LI Wen-hong, FANG Zhen-feng

Department of Aquaculture, Guangxi University, Nanning 530005, China

Received:2009-12-10 Revised:2009-12-24 Online:2010-12-15 Published:2010-12-15
About author:李文红(1966—), 女, 广西平果县人, 副教授, 博士, 从事研究藻类分子生物学。whli66@163.com
Supported by:
广西科学基金项目(桂科基0575008); 广西大学基金项目(X041120)

Abstract

Abstract:

The proper total DNA isolation methods of Gracilaria were chosen from three popular methods known as CTAB, SDS, and commercial Plant DNA Extraction kits by comparing their yield, quality, reliability, and the extraction time con-sumed. The total DNA was applied to restricted enzyme digestion and PCR amplification to detect its quality. The reactions of ingredients and programs of RAPD, ISSR, and ITS were constructed based on the detections. The results showed that the SDS method is the best method among the three for its highest yield and fine quality of total DNA, both good for fresh and frozen Gracilaria samples, not time-consuming and excellent reliability. The Plant DNA Extraction Kit was quick and user-friendly for the mass fresh samples DNA isolation in Gracilaria though its total DNA yield was low. The CTAB method was not rec-ommended for its time-consuming and poor reliability.

Key words: Gracilaria, total DNA isolation, PCR

LI Wen-hong,FANG Zhen-feng. DNA isolation and PCR amplified in Gracilaria[J].Journal of Tropical Oceanography, 2010, 29(6): 98-103.

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DNA isolation and PCR amplified in Gracilaria

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