川纹笛鲷染色体核型、银染和C-带

doi:10.11978/j.issn.1009-5470.2011.01.091

Journal of Tropical Oceanography ›› 2011, Vol. 30 ›› Issue (1): 91-95.doi: 10.11978/j.issn.1009-5470.2011.01.091cstr: 32234.14.j.issn.1009-5470.2011.01.091

• Marine biology • Previous Articles Next Articles

Studies on chromosome karyotype, Ag-NORs and C-banding patterns of Lutjanus sebae

GUO Ming-lan1, YOU Xin-xin2, SU Yong-quan2, DING Shao-xiong2, WANG Jun2

1. Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, CAS, Guangzhou 510301, China; 2. Department of Oceanography and Institute of Subtropical Oceanography, Xiamen University, Xiamen 361005, China

Received:2009-05-12 Revised:2010-06-22 Online:2011-03-17 Published:2011-03-16
Contact: 王军。E-mail: junw@xmu.edu.cn
About author:郭明兰(1980—), 女, 江西省吉安市人, 博士, 主要从事海洋动物生化遗传研究。E-mail: guominglan03@yahoo.com.cn
Supported by:
国家自然科学基金项目(30070595); 国家“863”计划项目(2006AA10A414)

Abstract

Abstract:

Studies on karyotype, Ag-NORs, and C-banding of Lutjanus sebae were performed. The chromosomes were re-ceived from the head kidney by using a method of injecting with PHA and colchicines, air drying, and Giemsa staining. The results were as follows. 1) The karyotypic formula of L.sebae was 2n=48t, NF=48. Meanwhile, a pair of chromosomes, with secondary constriction near the centromere, was found on chromosome 1. 2) The Ag-NORs polymorphisms were individually specific in this fish. The silver staining pots were 1-4, and the number of Ag-NORs was mainly 2 (79%). A pair of nucleolar organizer regions was observed on the secondary constriction of chromosome 1, and no Ag-NORs combination was found. 3) Centromeres of most chromosomes were C-bandings positive, and heterochromatin was detected at the satellite zone of chro-mosome 1. Thus, we discussed the evolution of karyotype, the developing mechanism of Ag-NORs, and C-bandings for fish. The phylogenetic condition of L. sebae was evaluated as well.

Key words: Lutjanus seba, karyotype, Ag-NORs, C-banding

GUO Ming-lan,YOU Xin-xin,SU Yong-quan,DING Shao-xiong,WANG Jun. Studies on chromosome karyotype, Ag-NORs and C-banding patterns of Lutjanus sebae[J].Journal of Tropical Oceanography, 2011, 30(1): 91-95.

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Studies on chromosome karyotype, Ag-NORs and C-banding patterns of Lutjanus sebae

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