南海野生斑节对虾亲虾卵巢发育过程中的脂肪酸组成

doi:10.11978/j.issn.1009-5470.2011.01.144

Abstract

Abstract:

Fatty acid (FA) composition of ovary, midgut gland and muscle of wild female Penaeus monodon from the northern South China Sea in different stages of ovary development was determined. Results showed that the main FAs were 16:0, 16:1n-7, 18:0, 18:1n-9, 20:4n-6 (arachidonic acid, ARA), 20:5n-3(eicosapntemacnioc acid, EPA) and 22:6n-3 (docosahex-aenoic acid, DHA). During sexual maturation, the percentage of 16:0 and total saturated FAs (saturation fatty acid, ∑SFA) in midgut gland increased fluctuantly. The percentage of 12:0, 14:0, 16:0, 18:0, 20:0 and ∑SFA in ovary increased (except for 22:0), and the highest percentage presented in Stage V and recovered to the level of immature stage after spawning. The per-centage of 14:0, 16:0, 18:0, 20:0 and ∑SFA in muscle decreased from Stage I to Stage IV or V, and increased after spawning. During sexual maturation, the percentage of 16:1n-7, 18:1n-9 and ∑MUFA (mono unsaturation fatty acid) in midgut gland, ovary, and muscle increased fluctuantly, and the highest percentage presented in Stage V. The percentage of 18: 2n-6, 20:4n-6 (ARA) and n-6 FA increased from Stage I to Stage III and decreased to the lowest level in Stage V. The percentage of 20:5n-3(EPA), 22:6n-3(DHA) and n-3 FA increased from Stage I to Stage III, decreased to the lowest level in Stage VI (except for EPA and n-3 FA), and decreased to the lowest level in Stage IV or V and increased after spawning. The ratios of DHA/EPA, n-3/n-6, and ∑PUFA (poly unsaturation fatty acid)/SFA increased fluctuantly from Stage I to Stage III or IV and decreased to the lowest level in Stage VI or recovered to the level of immature stage (Stage I) after spawning. The ratios of DHA/EPA, ∑n-3/n-6, and ∑PUFA/SFA in midgut gland were 0.5-1, 0.8-1.5, 0.29-0.53, respectively. Our results showed that the FA com-position in tissues of wild P. monodon broodstocks presented the balance between n-3 and n-6 FA families, ∑PUFA and ∑SFA, and EPA and DHA, and the ratio of ∑SFA:∑MUFA:∑PUFA was 35%:30%:35%. These results provide reference for making up the artificial diets for P. monodon broodstocks.

Key words: wild Penaeus monodon, ovary development, ovary, midgut gland, muscle, fatty acids

HUANG Jian-hua,YANG Qi-bin,LIN Hei-zhao,CHEN Xu,ZHOU Fa-lin,WEN Wei-geng,J. Fatty acid composition of wild female Penaeus monodon broodstock from the northern South China Sea during ovarian development[J].Journal of Tropical Oceanography, 2011, 30(1): 144-151.

References

[1] FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS. FAO FishStat Plus[EB/OL]. [2001-12-01]. http://www.fao. org.

[2] MOSS S M, CROCOS P J. Global shrimp OP: 2001 —preliminary report[R]. Maturation Report Glob Aquac Advocate, 2001.

[3] MENASVETA P, PIYATIRATITIVORAKUL S, RUNGSUPA S, et al. Gonadal maturation and reproductive performance of giant tiger prawn (Penaeus monodon Fabricius) from the Andaman Sea and pond-reared sources in Thailand[J]. Aquac, 1993, 116: 191-198.

[4] HANSFORD, S W, MARSDEN, G E. Temporal variation in egg and larval productivity of eyestalk ablated spawners of the prawn Penaeus monodon from Cook Bay[J]. Australia World Aquac Soc, 1995, 26: 396-400.

[5] COMAN G J, ARNOLD S J, PEIXOTO S, et al. Reproductive performance of reciprocally crossed wild-caught and tank reared Penaeus monodon broodstock[J]. Aquac, 2006, 252: 372-384.

[6] COMAN G J, ARNOLD S J, CALLAGHAN T R, et al. Effect of two maturation diet combinations on reproductive performance of domesticated Penaeus monodon[J]. Aquac, 2007, 263: 75-83.

[7] WOUTERS R, LAVENS P, NIETO J, et al. Penaeid shrimp broodstock nutrition: an updated review on research and development[J]. Aquac, 2001, 202: 1-21.

[8] JECKEL W H, DE MORENO J E A, MORENO V J. Biochemical composition, lipid classes and fatty acids in the ovary of the shrimp Pleoticus muelleri Bate[J]. Comp Biochem Physiol, 1989, 92B: 271-276.

[9] RAVID T, TIETZ A, KHAYAT M, et al. Lipid accumulation in the ovaries of a marine shrimp Penaeus semisulcatus (De Haan)[J]. J Exp Biol, 1999, 202(13): 1819-1829.

[10] WOUTERS R, MOLINA C, LAVENS P, et al. Lipid composition and vitamin content of wild female Litopenaeus vannamei in different stages of sexual maturation[J]. Aquac, 2001, 198: 307-323.

[11] MARSDEN G E, MC GUREN J J, HANSFORD S W, et al. A moist artificial diet for prawn broodstock: its effect on the variable reproductive performance of wild-caught Penaeus monodon[J]. Aquac, 1997, 149: 145-156.

[12] 季文娟, 徐学良. 中国对虾卵巢发育过程中脂肪酸组成的分析及比较研究[J]. 海洋水产研究, 1992(13): 7-12.

[13] XU X L, JI W J, CASTELL J D, et al. Influence of dietary lipid sources on fecundity, egg hatchability and fatty acid composition of Chinese prawn (Penaeus chinensis) broodstock[J]. Aquac, 1994, 119: 359-370.

[14] TESHIMA S, KANAZAWA A, KOSHIO S, et al. Lipid metabolism of the prawn Penaeus japonicus during maturation: Variation in lipid profiles of the ovary and hepatopancreas[J]. Comp Biochem Physiol, 1989, 92: 45-49.

[15] ALAVA V R, KANAZAWA A, TESHIMA S, et al. Effect of dietary phospholipids and n-3 highly unsaturated fatty acids on ovarian development of Kuruma prawn[J]. Nippon Suisan Gakkaishi, 1993, 59(7): 345-351.

[16] MILLAMENA O M. Effect of fatty acid composition of broodstock diet on tissue fatty acid patterns and egg fertilization and hatching in pond reared Penaeus monodon[J]. Asian Fish Sci, 1989, 2: 127-134.

[17] MILLAMENA O M, PASCUAL F P. Tissue lipid content and fatty acid composition of Penaeus monodon Fabricius broodstock from the wild [J]. J. World Aquac Soc, 1990, 21: 116-121.

[18] HUANG J H, JIANG S G, LIN H Z, et al. Effects of dietary highly unsaturated fatty acids and astaxanthin on fecundity and lipid content of pond-reared Penaeus monodon (Fabricius) broodstock[J]. Aquac Res, 2008, 9: 240-251.

[19] 黄建华, 周发林, 林黑着, 等. 池塘养殖斑节对虾卵巢发育过程中的脂肪酸组成及变化[J]. 上海水产大学学报, 2007, 16(4): 341-349.

[20] 黄建华, 周发林, 马之明, 等. 南海北部斑节对虾卵巢发育的形态及组织学观察[J]. 热带海洋学报, 2006, 25(3): 47-52.

[21] 黄建华, 马之明, 周发林, 等. 南海北部野生斑节对虾卵巢解剖结构及组织学的研究[J]. 南方水产,2005, 1(3): 49-53.

[22] LIN H Z, LI Z J, CHEN Y Q, et al. Effect of dietary traditional Chinese medicines on apparent digestibility coefficients of nutrients for white shrimp Litopenaeus vannamei, Boone[J]. Aquac, 2006, 253: 495-501.

[23] PALACIOS E, IBARRA A M, RACOTTA I S. Tissue biochemical composition in relation to multiple spawning in wild and pond-reared Penaeus vannamei broodstock[J]. Aquac, 2000, 185: 353-371.

[24] O’LEARY C D, MATHEWS A D. Lipid class distribution and fatty acid composition of wild and farmed prawn, Penaeus monodon_Fabricius[J]. Aquac, 1990, 89: 65-81.

[25] MIDDLEDITCH B S, MISSLER S R, HINES H B, et al. Metabolic profiles of penaeid shrimp: dietary lipids and ovarian maturation[J]. J Chromatogr, 1980, 195: 359-368.

[26] PALACIOS E, RACOTTA I S, HERAS H, et al. Relation between lipid and fatty acid composition of eggs and larval survival in white pacific shrimp (Penaeus vannamei, Boone, 1931) [J]. Aquaculture International, 2001, 9: 531-543.

[27] CAVALLI R O, LAVENS P, SORGELOOS P. Performance of Macrobrachium rosenbergii broodstock fed diets with different fatty acid composition[J]. Aquac, 1999, 179: 387-402.

[28] LYTLE J S, LYTLE T S, OGLE J T. Polyunsaturated fatty acid profiles as a comparative tool in assessing maturation diets of Penaeus vannamei[J]. Aquac, 1990, 89: 287-299.

Fatty acid composition of wild female Penaeus monodon broodstock from the northern South China Sea during ovarian development

PDF (PC)

Like

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 5

Metrics

Comments

Recommended 0

[1]	WU Xueping, YE Hongxia, YAO Youju, LIU Haoxiang, LI Ruihua, TONG Tong. Analysis and evaluation of nutrient composition and heavy metal content of Phascolosama esulenta in three regions [J]. Journal of Tropical Oceanography, 2024, 43(2): 92-107.
[2]	LIAN Changpeng, WU Tao, WANG Chaoqi, YANG Ling, PAN Ying. Ovarian development, histology of oogenesis and yolk formation of Tapes conspersus from the Beihai Yingpan, Guangxi [J]. Journal of Tropical Oceanography, 2022, 41(5): 170-179.
[3]	CHEN Lingzhen, QIN Geng, WANG Xin, LIU Yali, XIAO Wanghong, LIN Qiang. Distinct structures of gonads and germ cell development of lined seahorse, Hippocampus erectus [J]. Journal of Tropical Oceanography, 2020, 39(6): 93-102.
[4]	YANG Xian-qing,SUN Man-yi,CEN Jian-wei,LI Lai-hao,WU Yan-yan,et al. Elimination of malachite green and its metabolite in tilapia muscle [J]. Journal of Tropical Oceanography, 2010, 29(4): 107-111.
[5]	CAO Fu-jun. Observation on the ovary development of Scartelaos virids [J]. Journal of Tropical Oceanography, 2009, 28(6): 123-129.