广东省粤西地区凡纳滨对虾 3 种主要病毒的 PCR 与 LAMP 检测及流行病学特点分析

doi:10.11978/j.issn.1009-5470.2011.06.097

Abstract

Abstract: In order to investigate the infected rates of White Spot Syndrome Virus (WSSV), Taura Syndrome Virus (TSV) and Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) among aquatic Litopenaeus vannamei, we tested the samples from western Guangdong Province using Polymerase Chain Reaction (PCR) and Loop-mediated Isothermal Amplication (LAMP). The results show that there were higher infected rates of WSSV and TSV, which suggests these two viruses were the dominant pathogens in this region. These data reveal that the LAMP method was equivalent to the PCR method in specificity and sensitivity, but the LAMP method was more convenient, rapid and low cost than the PCR.

Key words: Litopenaeus vannamei, White Spot Syndrome Virus, Infectious Hypodermal and Hematopoietic Necrosis Virus, Taura Syndrome Virus, detection

CLC Number:

P735

JIANG Xiao,REN Chun-hua,HU Chao-qun,ZHAO Zhe . Epidemiological investigation of three kinds of major virus in Litopenaeus vannamei from western Guangdong Province[J].Journal of Tropical Oceanography, 2011, 30(6): 97-103.

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Epidemiological investigation of three kinds of major virus in Litopenaeus vannamei from western Guangdong Province

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