雷州半岛近岸海域海洋红酵母的分离鉴定

doi:10.11978/2016117

Abstract

Abstract:

In this study, 317 samples from marine organisms, seawater and sea mud were gathered in a coastal area near the Leizhou Peninsula. After basic process and enrichment culture, marine Rhodotorula was isolated by using Rhodotorula agar medium and wort agar medium. The Rhodotorula strains were preliminary identified by using cell morphology characteristics, cultural characteristics, physiological and biochemical test results. Based on the preliminary identification results, representative strains were tested by 26s rDNA D1/D2 domain sequence analysis, phylogenetic tree was constructed and diversity was analyzed. The 71 strains isolated from 317 samples were preliminary identified as marine Rhodotorula among which 36 representative strains were identified as belong to five species and two suspected to be new species. Among them, 22 strains were R. mucilaginosa, six strains were R. glutinis, three strains were R. toruloides, two strains were R. slooffiae, one strain was R. pallida, and two strains were suspected to be new species named Rhodotorula sp. cZDJ2 and Rhodotorula sp. ZTC2, respectively. The dominant species of marine Rhodotorula in the coastal area near the Leizhou Peninsula was Rhodotorula mucilaginosa, indicating that the diversity of population in the coastal area near the Leizhou Peninsula was not rich enough.

Key words: Marine Rhodotorula, Isolation and Identification, Coastal area, Leizhou Peninsula

CLC Number:

Q93-331

Jiannan SUN, Ying LIU, Weitian XIE, Chunhou XU. Isolation and identification of marine Rhodotorula in a coastal area near Leizhou Peninsula[J].Journal of Tropical Oceanography, 2017, 36(4): 87-92.

Figures/Tables 5

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References 16

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分离菌株	蔗糖	麦芽糖	海藻糖	松三糖	木糖	鼠李糖	乳糖	蜜二糖	棉子糖	甘露糖	菊糖	肌醇	淀粉	硝酸盐	亚硝酸盐	乙胺	肌酸	肌酸酐
ZTC4等46株	+	+	+	+	+	v	-	-	+	v	-	-	-	-	-	+	-	-
aZDD4等12株	+	+	+	+	v	+	-	-	+	+	+	-	-	+	+	+	-	-
ZTHD2等6株	+	+	+	+	+	-	-	-	v	v	v	-	-	+	+	v	-	-
aZDB等4株	+	-	+	+	+	v	+	-	v	v	v	-	-	+	+	-	-	-
ZZC2	+	-	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-
ZTC2	-	+	+	-	+	+	-	-	+	+	-	-	-	+	+	+	-	-
cZDJ2	+	-	+	+	+	-	-	-	-	+	-	-	-	-	-	-	-	-

类群	分离菌株(Genbank登录号)	相似性最高模式菌株	相似性/%
R. mucilaginous (22株)	ZZC1(KP979796)、aZDD6(KP954615)	R. mucilaginosa ATCC 32763	99.6
	ZTHD4(KP954623、ZTHG1(KP954624)
	aZDY(KP966487)、ZTHY5(KP966488)
	ZTHQ(KP954629)
	cZDL1(KP954617)、aZDY1(KP954618)	R. mucilaginosa ATCC 32763	99.5
	aZDZ1(KP954619)、cZDY5(KP954620)
	bZDB2(KP966490)、 ZDS(KP954630)
	ZTHY1(KP954625)	R. mucilaginosa ATCC 32763	99.3
	aZDD3(KP979795)	R. mucilaginosa ATCC 32763	99.1
	cZDY2(KP954616)、ZTHD3(KP954622)	R. mucilaginosa CBS 8383	99.6
	ZTC4(KP966489)	R. mucilaginosa CBS 8383	99.5
	ZTHY4(KP954626)、ZTC3(KP954627) ZTC5(KP954628) 、ZTHY2(KP966486)	R. mucilaginosa KCTC 2829	99.6
R. glutinis(6株)	cZDD5(KP979800)、 bZDD5(KP979801) bZDD4(KP979802) 、bZDD1(KP979803) aZDD5(KP979804)、aZDD4(KP979805)	R. glutinis ATCC 32765	99.3
R. toruloides(3株)	ZTHD2(KP979806)、ZTC1(KP979807)	R. toruloides KCTC 17083	99.5
R. toruloides(3株)	ZTHD1(KP979808)	R. toruloides KCTC 17083	99.1
R. slooffiae(2株)	aZDH8(KP979798)、aZDB(KP979799)	R. slooffiae CBS 5706	99.5
R . pallid(1株)	ZZC2(KP979797)	R. pallid CBS 320	99.3
Rhodotorula sp.(1株)	cZDJ2(KP979809)	R. minuta JCM 5951	93.0
Rhodotorula sp.(1株)	ZTC2(KP954620)	R. mucilaginosa ATCC 32763	98.7

Isolation and identification of marine Rhodotorula in a coastal area near Leizhou Peninsula

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