核酸指标对桡足类安氏伪镖水蚤摄食率、生长率及产卵率的指示作用

doi:10.11978/2017053

Abstract

Abstract:

Nucleic acid indices can indicate nutrition status and growth of copepods; yet, lack of comparison among different phylogenetic groups and indices constrains application of nucleic acid proxies in field investigation. In this study, ingestion rate, growth rate of copepodid at Stage Ⅱ and egg production rate were studied in brackish copepod Pseudodiaptomus annandalei at different food levels (0.2~2.0 μg C·mL^-1). Biochemical indices including mass specific RNA content, DNA content, RNA:DNA, and RNA: protein ratios, as well as elemental composition (mass specific phosphorus content, nitrogen content, atomic C:P, and C:N ratios ), were also measured and described. Ingestion rate, growth rate, egg production rate, and RNA-related indices in copepodids increased with increasing food level hyperbolically, so did RNA:DNA ratio in female adults. Ingestion and growth rates were significantly correlated with RNA-related indices in copepodids in a linear manner and the strength of correlation ranked as RNA:DNA>RNA:PRO>RNA content. Egg production rate, however, was correlated with RNA:DNA and RNA:PRO ratios in female adults with high and moderate significance, respectively. Our results suggested that RNA:PRO ratio is suitable to be an indicator of growth and reproduction rate in copepod besides RNA:DNA ratio and RNA content, which have been applied in field investigation. However, RNA:DNA as proxy of somatic growth should be confined to the same-aged copepod. In addition, the finding of hyperbolic correlation between RNA-related indices and food concentration implied that they may help prediction of food environment for copepod, but may be confined to food levels at unsaturated phase of functional responses of variables.

Key words: nucleic acid indicators, copepod, ingestion, somatic growth, reproduction

Xuejia HE, Huaming YANG, Baidong LYU, Jianqiang YIN. Nucleic acid proxies for ingestion rate, growth and reproduction in copepod Pseudodiaptomus annandalei[J].Journal of Tropical Oceanography, 2018, 37(2): 26-35.

Figures/Tables 7

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References 20

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生长阶段	指标	y_max	b	c	r	p
桡足幼体	摄食率	0.053±0.006	2.04±0.62	0	0.95	0.012
	生长率	0.16±0.0053	3.3±0.34	0	0.99	0.001
	RNA:干重	0.083±0.0019	8.1±0.85	0	0.97	0.0054
	RNA:PRO	0.14±0.0008	11±0.37	0	0.99	<0.001
	RNA:DNA	15±3.3	0.56±0.38	1.1±0.56	0.96	<0.01
雌性成体	产卵率	6.8±0.34	2.6±0.38	0	0.99	<0.01
	RNA:DNA	15±0.26	2.6±0.82	0.56±0.20	0.97	<0.001
	C:P	434±15	7.7±1.2	0	0.95	0.015

生长阶段	生理指标	核酸指标和元素组成	Pearson分析		y=ax+b
生长阶段	生理指标	核酸指标和元素组成	p	r	a	b
桡足Ⅱ期	摄食率	RNA︰干重	0.018*	0.725	0.454	0.065
		RNA︰DNA	0.000**	0.953	114.8	6.956
		RNA︰PRO	0.003**	0.836	3.815	-0.029
		P含量/%	0.509	0.237	-0.443	0.320
		N含量/%	0.797	0.093	1.05	3.957
		C:P	0.510	0.237	155.1	103.5
		C:N	0.857	0.066	-1.461	8.327
桡足Ⅱ期	生长率	RNA︰干重	0.005**	0.688	0.165	0.074
		RNA︰DNA	0.000**	0.814	39.60	4.942
		RNA︰PRO	0.004**	0.702	1.118	-0.064
		P含量/%	0.419	0.225	0.158	0.281
		N含量/%	0.307	0.238	1.212	3.812
		C:P	0.503	0.187	-48.443	116.13
		C:N	0.528	0.178	-1.515	8.514
雌性成体	产卵率	RNA︰干重	0.076	0.636	0.039	-0.001
		RNA︰DNA	0.000**	0.851	0.511	11.71
		RNA︰PRO	0.027*	0.565	3.815	-0.029
		P含量/%	0.000**	0.891	-0.019	0.303
		N含量/%	0.193	0.356	0.244	2.593
		C:P	0.000**	0.889	12.29	88.81
		C:N	0.132	0.408	-0.395	11.79

Nucleic acid proxies for ingestion rate, growth and reproduction in copepod Pseudodiaptomus annandalei

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