Abstract: Lipid and fatty acid contents were firstly monitored in the reef coral Acropora hyacinthus during its reproductive cycle by using chloroform-methanol mixture extraction method and gas chromatography-mass spectrometry. The results are summarized here. The lipid content and tissues of the reef coral A. hyacinthus fluctuated markedly during the reproductive process, with the lipid content decreased drastically by approximately 43.4% and the tissues reduced by about 19.6% after reproduction. However, there was no significant difference in symbiotic algae density during the breeding process, implying that the eggs were probably without zooxanthellae. The reef coral A. hyacinthus accumulated lipid in the process of reproduction preparing. EPA (C20:5n-3, eicosapntemacnioc acid) and DHA (C22:6n-3, docosahexaenoic acid) played important roles in reproduction, since their contents changed significantly during this process. EPA and DHA increased by 41.3% and 36.5%, respectively, before breeding. However, their contents decreased by 32.0% and 34.3%, respectively, after breeding. Meanwhile, the content of poly-unsaturated fatty acids (PUFAs) also reduced appreciably, suggesting that they were intimately bound up with the reproduction. Scleractinian corals likely increased feeding to meet the need of fatty acids and energy during the reproductive process.