Journal of Tropical Oceanography ›› 2018, Vol. 37 ›› Issue (6): 112-119.doi: 10.11978/2018016CSTR: 32234.14.2018016

• Marine Biology • Previous Articles     Next Articles

Whole-genome sequencing and analysis of marine Bdellovibrio-and-like organism DA5

Youyun LU(), Ming XUE, Zhihua LI, Chongqing WEN()   

  1. Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China
  • Received:2018-02-06 Online:2018-11-20 Published:2018-12-24
  • Supported by:
    National Natural Science Foundation of China (31372536);Science and Technology Planning Project of Guangdong Province (2015A020209160)

Abstract:

To explore and utilize marine Bdellovibrio-and-like organisms (BALO) and their genes, the whole genome of Halobacteriovorax sp. DA5 was sequenced using the Illumina HiSeq platform. Then, fragment assembly, gene prediction and functional annotation were analyzed in comparison with those of other eight BALO strains. The results showed that the genome size of strain DA5 is 3.27 Mb with GC content of 36.5%, and encodes 3175 predicted genes, including 303 genes found in the Clusters of Orthologous Groups (COG) database and 1239 genes related to metabolic pathways. Comparative genomic analysis showed that DA5 has typical characteristics identified in genomes of the marine BALOs, and there were 467 homologous gene families common in genomes of DA5 and other eight BALOs, while 266 unique genes were found only in DA5 genome. Among all the 62 genes associated with cell motility of strain DA5, members such as methyl-accepting chemotaxis protein, putative flagellin, flagellar motor proteins, and flagellar motor switch proteins were most affiliated phylogenetically with those of Halobacteriovorax sp. BAL6_X. Therefore, annotation and functional analysis of the genome sequence of DA5 provide insight into its predatory property and interaction mechanism with host, and establish the basis for DA5 as biocontrol agent against harmful bacteria occurring in marine aquaculture.

Key words: marine Bdellovibrio-and-like organisms, Halobacteriovorax, whole-genome sequencing, gene function, comparative genomic analysis

CLC Number: 

  • Q933