Journal of Tropical Oceanography >
Noctiluca scintillans effects on eukaryotic plankton community structure using Environmental DNA analysis in Daya Bay*
Copy editor: YIN Bo
Received date: 2022-01-10
Revised date: 2022-04-05
Online published: 2022-09-30
Supported by
National Natural Science Foundation of China(41776154)
Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory(GML2019ZD0405)
Noctiluca scintillans is a key blooms species and widely distributed throughout the world. Previous studies indicated that the plankton community was strongly influenced by the predation of N. scintillans. In this study, eukaryotic plankton community and biological correlation in the Daya Bay were analyzed using environmental DNA technology and Spearman correlate analysis in January 2021. The temperature and salinity of surface sea water were 14.4 ~ 18.3 ℃ and 32.52 ‰ ~ 33.14 ‰, respectively. In the stations with high N. scintillans abundance, the relative abundance of phytoplankton, such as Chattonellaceae and Thalassiosiraceae were 2.41 % and 2.23 %, which is significantly higher than the average values of 0.75 % and 0.62 % at other stations, respectively. While the relative abundances of Paracalanidae and Oithonidae were 8.22 % and 4.46 % at high N. scintillans abundance stations, which is significantly lower than the average value of 64.13 % and 11.12 % at other stations, respectively. These results indicated that N. scintillans reduced the top-down control of Paracalanidae on phytoplankton including Chattonellaceae, Thalassiosiraceae, Prorocentraceae and Geminigeraceae which led to an increase in the abundance of some plankton species in the blooms areas. Meanwhile, Gymnodiniaceae and Stephanopyxidaceae were fed by N. scintillans, which directly affected the community abundance of phytoplankton. Our analysis suggests that eDNA technology provides the methodological basis for understanding the ecological niche of N. scintillans, which would ultimately contribute to understanding the occurrence of blooms strategies.
CHEN Jingfu , ZHONG Yu , WANG Lei , GUO Yupei , QIU Dajun . Noctiluca scintillans effects on eukaryotic plankton community structure using Environmental DNA analysis in Daya Bay*[J]. Journal of Tropical Oceanography, 2022 , 41(5) : 121 -132 . DOI: 10.11978/2022005
图2 大亚湾环境因子空间分布a. 温度; b. 盐度; c. 磷酸盐浓度; d. 硅酸盐浓度; e. 硝酸盐浓度; f. 亚硝酸盐浓度; g. 溶解氧浓度。审图号为GS(2019)4342 Fig. 2 Distribution of physical and chemical data collected in the Daya Bay. (a) temperature; (b) salinity; (c) orthophosphate (PO43-); (d) dissolved silicate (SiO44-); (e) nitrate (NO3-); (f) nitrite (NO2-); (g) dissolved oxygen (DO) |
图4 大亚湾各站位(S1~S12)环境DNA样品扩增真核生物的稀释曲线图a. 为粒径≥3µm环境DNA样品; b. 为粒径0.2~3µm环境DNA样品 Fig. 4 Rarefaction curves of eDNA sampling of eukaryotes in the Daya Bay. (a) size ≥ 3 µm; (b) size is 0.2 ~ 3 µm |
表1 大亚湾浮游生物群落高通量测序结果Tab. 1 Summary of the high-throughput sequencing reads of eDNA in the Daya Bay |
站位 | 原始序列数 | 有效序列数 | 有效比 | OTUs数 |
---|---|---|---|---|
S1≥3µm | 85852 | 83035 | 96.72 | 451 |
S1-pico | 86846 | 82146 | 94.59 | 662 |
S2≥3µm | 86414 | 83728 | 96.89 | 252 |
S2-pico | 80261 | 76850 | 95.75 | 930 |
S3≥3µm | 87818 | 84321 | 96.02 | 164 |
S3-pico | 85984 | 82880 | 96.39 | 464 |
S4≥3µm | 82743 | 79504 | 96.09 | 340 |
S4-pico | 85679 | 82797 | 96.64 | 474 |
S5≥3µm | 87153 | 84519 | 96.98 | 212 |
S5-pico | 87939 | 84033 | 95.56 | 509 |
S6≥3µm | 83419 | 80163 | 96.10 | 251 |
S6-pico | 85651 | 82202 | 95.97 | 582 |
S7≥3µm | 86571 | 83124 | 96.02 | 398 |
S7-pico | 82757 | 80002 | 96.67 | 253 |
站位 | 原始序列数 | 有效序列数 | 有效比 | OTUs数 |
S8≥3µm | 80444 | 77315 | 96.11 | 397 |
S8-pico | 81353 | 76092 | 93.53 | 799 |
S9≥3µm | 80922 | 77302 | 95.53 | 638 |
S9-pico | 81500 | 78682 | 96.54 | 345 |
S10≥3µm. | 87029 | 83148 | 95.54 | 618 |
S10-pico | 82076 | 77528 | 94.46 | 619 |
S11≥3µm | 82660 | 79646 | 96.35 | 223 |
S11-pico | 85237 | 81181 | 95.24 | 527 |
S12≥3µm | 85806 | 82684 | 96.36 | 212 |
S12-pico | 86122 | 81857 | 95.05 | 624 |
图5 真核浮游生物群落丰度图a、c、e、g为粒径≥3µm环境DNA样品中真核浮游生物在不同分类阶元丰度图; b、d、f、h 为粒径0.2~3µm环境DNA样品中真核浮游生物在不同分类阶元丰度图。a、b为真核生物; c、d为纲水平; e、f为目水平; g、h为科水平 Fig. 5 The relative abundance of eukaryotes at different taxonomic levels. (a, c, e, g) showing eukaryotes relative abundance (eukaryote, class, order, family) in ≥ 3 µm sizes eDNA samples, respectively; (b, d, f, h) showing eukaryotes relative abundance (eukaryote, class, order, family) in 0.2 ~ 3 µm sizes eDNA samples, respectively |
图6 浮游植物群落丰度图a、c、e、g为环境DNA中粒径≥3µm真核浮游植物在不同分类阶元的丰度图; b、d、f、h为环境DNA中粒径0.2~3µm真核浮游植物在不同分类阶元的丰度图。a、b为真核生物; c、d为纲水平; e、f为目水平; g、h为科水平 Fig. 6 Relative abundance of phytoplankton at different taxonomic levels. (a, c, e, g) showing the phytoplankton relative abundance (eukaryote, class, order, family) in ≥ 3 µm sizes eDNA samples, respectively; (b, d, f, h) showing the phytoplankton relative abundance (eukaryote, class, order, family) in 0.2 ~ 3 µm sizes eDNA samples, respectively |
图7 浮游生物群落网络关系图黄色实线表示正相关关系, 橙色实线表示与夜光藻呈正相关关系, 灰色虚线表示负相关关系, 线的粗细代表关系的强弱程度 Fig. 7 Network of plankton community. Yellow solid line means positive correlation; Orange solid line means positive correlation to N. scintillans; Grey dotted line means negative correlation; thickness of the line represents the strength of the relationship |
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