Journal of Tropical Oceanography >
Oligophenalenone dimers with anti-inflammatory activities from fungus Talaromyces stipitatus
Copy editor: LIN Qiang
Received date: 2023-02-03
Revised date: 2023-03-21
Online published: 2023-03-24
Supported by
National Natural Science Foundation of China(81903772)
An extract of the fungus Talaromyces stipitatus showed inhibitory activity against the nitric oxide (NO) production in the lipopolysaccharide (LPS)-activated RAW264.7 macrophage cell line, and bioassay-guided separation of the extract provided six oligophenalenone dimers, which were characterized to be bacillisporin A (1), 9a-epi-bacillisporin E (2), bacillisporin F (3), duclauxin (4), bacillisporin B (5) and bacillisporin C (6), respectively, by comparison of the nuclear magnetic resonance (NMR) and high resolution electrospray ionization mass spectroscopy (HRESIMS) data with literature data. 2-5 were noncytotoxic on macrophage cells at 30 µmol·L-1, and displayed inhibitory effects against NO production in LPS-induced RAW264.7 macrophage cells, which were all more active than the positive control indomethacin (AG). The half inhibitory concentration (IC50) values of 2-4 were 11.82±1.25, 11.44±1.58 and 23.92±2.86 µmol·L-1, respectively. 5 and 6 were isolated from T. stipitatus for the first time. Duclauxin and its homologues were firstly discovered to show significant anti-inflammatory activities in vitro, which provide a new structural model for further research of anti-inflammatory activity.
ZHU Ling , PAN Chao , HUA Silu , JIANG Wei . Oligophenalenone dimers with anti-inflammatory activities from fungus Talaromyces stipitatus[J]. Journal of Tropical Oceanography, 2023 , 42(6) : 150 -155 . DOI: 10.11978/2023012
表1 化合物2—6对LPS诱导的RAW264.7细胞NO生成的抑制活性 ($\bar{x} \pm s$, n = 3)Tab. 1 Inhibitory activities of compounds 2-6 against NO production in RAW264.7 cells induced by LPS ($\bar{x} \pm s$, n = 3) |
化合物 | 抑制率/%a | IC50/(µmol·L-1) |
---|---|---|
2 | 92.40±6.48 | 11.82±1.25 |
3 | 90.85±6.15 | 11.44±1.58 |
4 | 74.08±5.04 | 23.92±2.86 |
5 | 44.70±3.42 | >30 |
6 | 9.67±1.06 | >30 |
阳性对照(AG) | 36.71±5.23 | >30 |
注: a测试样品浓度为30.00µmol·L-1 |
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