真菌Talaromyces stipitatus 中具有抗炎活性oligophenalenone二聚体

doi:10.11978/2023012

Abstract

Abstract:

An extract of the fungus Talaromyces stipitatus showed inhibitory activity against the nitric oxide (NO) production in the lipopolysaccharide (LPS)-activated RAW264.7 macrophage cell line, and bioassay-guided separation of the extract provided six oligophenalenone dimers, which were characterized to be bacillisporin A (1), 9a-epi-bacillisporin E (2), bacillisporin F (3), duclauxin (4), bacillisporin B (5) and bacillisporin C (6), respectively, by comparison of the nuclear magnetic resonance (NMR) and high resolution electrospray ionization mass spectroscopy (HRESIMS) data with literature data. 2-5 were noncytotoxic on macrophage cells at 30 µmol·L^-1, and displayed inhibitory effects against NO production in LPS-induced RAW264.7 macrophage cells, which were all more active than the positive control indomethacin (AG). The half inhibitory concentration (IC₅₀) values of 2-4 were 11.82±1.25, 11.44±1.58 and 23.92±2.86 µmol·L^-1, respectively. 5 and 6 were isolated from T. stipitatus for the first time. Duclauxin and its homologues were firstly discovered to show significant anti-inflammatory activities in vitro, which provide a new structural model for further research of anti-inflammatory activity.

Key words: Talaromyces stipitatus, oligophenalenone dimer, anti-inflammatory activity, duclauxin homologues, fungus

ZHU Ling, PAN Chao, HUA Silu, JIANG Wei. Oligophenalenone dimers with anti-inflammatory activities from fungus Talaromyces stipitatus[J].Journal of Tropical Oceanography, 2023, 42(6): 150-155.

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化合物	抑制率/%^a	IC₅₀/(µmol·L^-1)
2	92.40±6.48	11.82±1.25
3	90.85±6.15	11.44±1.58
4	74.08±5.04	23.92±2.86
5	44.70±3.42	>30
6	9.67±1.06	>30
阳性对照(AG)	36.71±5.23	>30

Oligophenalenone dimers with anti-inflammatory activities from fungus Talaromyces stipitatus

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