In order to investigate the secondary metabolites of the fungal-bacterial symbiont Aspergillus spelaeus GXIMD 04541/Sphingomonas echinoides GXIMD 04532 derived from Mauritia Arabica, compounds were isolated and purified by silica gel chromatography, sephadex LH-20 gel column chromatography, and semi-preparative high performance liquid chromatography (HPLC). The structures of the compounds were identified by modern spectroscopic methods and compared with relevant literature data. Cytotoxic activity evaluation of the compounds was conducted using MTT assay. Consequently, seven compounds were obtained from rice fermentation extracts of symbiont GXIMD 04541/04532, which were identified as trichalasins C (1), aspochalasin E (2), aspochalasin H (3), aspochalasin I (4), aspochalasin K (5), citreofuran (6), and cyclothiocurvularin B methyl ester (7). The results of the activity tests demonstrated that compounds 2, 4 and 5 exhibited cytotoxic activity against PC3 cells, with IC50 values of 17.23, 15.18 and 8.71 μM, respectively. Howerver, compounds 1-5 didn’t show cytotoxicity against 22Rv1 cells. This study enrichs the chemical diversity in metabolites derived from marine fungal-bacterial symbiont sources.